Hydroxypyridinium Crosslinks HPLC Column
The Hydroxypyridinium Crosslinks HPLC Column is For Research Use Only
Each column is can be utilized for approximately 500-1000 samples depending on column care and sample type.
Assay Background
Pyridinium and deoxypyridinium are built as intramolecular crosslinkers during the maturation of collagen. During bone resorption collagen is degraded and the pyridinium-crosslinks are excreted. They are not re-utilized by the body and excreted in the urine. The crosslinks are not released during the de novo synthesis of collagen. They are specific for resorptive processes. Neither PYD nor DPD are taken up by nutrition. Therefore the determination of the crosslinks is independent from the diet. PYD is found in cartilage, ligaments and bone, whereas DPD is rather specific for bone.
The crosslink excretion shows a strong circadian rhythm with a maximum in the early morning hours. Reliable data can be obtained with a 24h or a creatinine corrected morning urine. An elevated crosslink excretion is shown in osteoporotic women. Crosslink levels decrease during treatment with estrogen (Seibel et al. 1994). Therefore they are suited for therapy monitoring (Ganero et al. 1994). The measurement of pyridinium crosslinks is an independent predictor for future bone fractures (Ganero et al. 1995). Increased crosslink concentrations are also described in patients with Paget’s disease, primary hyperparathyroidism and bone metastasis.
Hydroxypyridinium Crosslinks HPLC Assay kit application for the pyridinium crosslinks makes it possible to determine pyridinium and deoxypyridinium in an easy, fast and precise method. The Hydroxypyridinium Crosslinks HPLC Assay kit includes all reagents in a ready to use form for preparation and separation of the samples with exception of the columns (IC3201rp) and the controls (IC3201ko). Both can be supplied separately by Eagle Biosciences. Beside the complete test kits it is possible to order all components separately. Please request our single component price list.
Related Products
Hydroxypyridinium Crosslinks HPLC Assay
Hydroxypyridinium Crosslinks HPLC Control Set
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需要做脂肪代谢实验，打算通过在心肌新鲜组织匀浆中加入核素标记的脂肪酸代谢底物（[14C]-Palmitate），通过液体闪烁计数仪测定氧化产物[14C]CO2的放射性计数来反应脂肪酸的氧化代谢率。但是实验室只有γ放射免疫计数仪，请问能否用其测量“软β射线”14C；仪器需要作何调整吗？？？
因为声的多普勒效应，用专用仪器在空气里测频率似乎变得不可靠。 最简单直接的方法，用频率特性仪，接换能器正负极测量读数 或者拿一片超声波振子接受对方发射的超声波进行放大后，输入到整形电路，再输入到计数器，就可以测量频率了
频率，是单位时间内完成周期性变化的次数，是描述周期运动频繁程度的量，常用符号f或ν表示，单位为秒分之一，符号为s。为了纪念德国物理学家赫兹的贡献，人们把频率的单位命名为赫兹，简称"赫"，符号为Hz。每个物体都有由它本身性质决定的与振幅无关的频率，叫做固有频率。频率概念不仅在力学、声学中应用，在电磁学、光学与无线电技术中也常使用。
能不能用kappa检验，我看了论坛上的帖子，好像是不行的。但是看到一篇文章，计数资料却用了kappa检验，请问能否这样使用，如果能的话，应该用什么软件，怎么计算。谢谢
提到的文章见后
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缺点是人工计数可能发生的随机误差较大。
1、同样的测量条件下，如测量相对几何位置相同，放射源相同，放射性测量计数率扣除本底后与活度在统计误差内成正比；
2、同样活度不同核素放射源，在其它条件相同的情况下放射性测量计数率不一定相同。因为不同核素衰变各能量分支比不同、粒子不同、能量不同，有的衰变子体也有放射性。
3、放射性测量计数率与计数器的探测效率相关。简单认为：活度＝（计数率-本底计数率）/效率。其中效率的标定要与测量条件一致。
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别告诉我计数的方法用数，我今天早上数了0.4g的微丸，近1000粒，快成熊猫，差点被送动物园了。也别告诉我测微丸的粒径用尺子量，我可是想着把它们排成一条线（增加准确度，可以避免忽略小粒子）再量，可是，这些小粒子不像《花园宝宝》里的小点点们那样听话，他们不爱排队。
各位老师是否还有什么高招？小的在这请教了。
