Highlights
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready:Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased:Complete RNA recovery without miRNA loss.
Description
| Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. |
|---|---|
| Equipment | Microcentrifuge, vortex |
| Sample Inactivation | TRI Reagent® (provided with R2051, R2053) inhibits RNase activity and inactivates viruses and other infectious agents. |
| Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). |
| Size Range | Total RNA ≥ 17 nt |
| Yield | 50 µg RNA (binding capacity), ≥25 µl (elution volume) |
Q1: Is DNase I available for individual purchase?
All kit components are available for purchase separately.
Q2: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q3: Is the kit compatible with samples stored in DNA/RNA Shield?
Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.
Q4: Is Direct-zol suitable for very small numbers of cells?
Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)
Q5: Is it possible to extract proteins with the Direct-zol RNA kits?
Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.
Q6: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?
Direct-zol DNA/RNA (D2080) kits can isolate DNA from TRIzol
Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.
Q10: Which phenol-based reagents are compatible with Direct-zol?
The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.
Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.
Q13: I ran out of RNA Wash Buffer. Can I use something else?
Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”
-Adina B. (University of Guelph)
“This kit is amazing, I"ve got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“
-R.K. CSU
“Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”
-H.Z. (Harvard Medical School)
Read More| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| E1010-1-4 | DNA Digestion Buffer | 4 mL | $15.00 | |
| E1010-1-16 | DNA Digestion Buffer | 16 mL | $29.00 | |
| R2050-1-200 | TRI Reagent | 200 ml | $219.00 | |
| R2050-1-50 | TRI Reagent | 50 ml | $70.00 | |
| C1078-50 | Zymo-Spin IICR Columns | 50 Pack | $55.00 | |
| R1003-3-12 | RNA Wash Buffer | 12 ml | $30.00 | |
| R1003-3-48 | RNA Wash Buffer | 48 ml | $105.00 | |
| E1010 | DNase I Set | 250 U | $56.00 | |
| R2050-2-40 | Direct-zol RNA PreWash (Concentrate) | 40 ml | $42.00 | |
| R2050-2-160 | Direct-zol RNA PreWash (Concentrate) | 160 ml | $166.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
| R2050 | Direct-zol RNA Miniprep | 50 preps | $180.00 | |
| R2052 | Direct-zol RNA Miniprep | 200 preps | $555.00 | |
| W1001-6 | DNase/RNase-Free Water | 6 ml | $15.00 | |
| W1001-30 | DNase/RNase-Free Water | 30 ml | $22.00 |
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需要做脂肪代谢实验，打算通过在心肌新鲜组织匀浆中加入核素标记的脂肪酸代谢底物（[14C]-Palmitate），通过液体闪烁计数仪测定氧化产物[14C]CO2的放射性计数来反应脂肪酸的氧化代谢率。但是实验室只有γ放射免疫计数仪，请问能否用其测量“软β射线”14C；仪器需要作何调整吗？？？
因为声的多普勒效应，用专用仪器在空气里测频率似乎变得不可靠。 最简单直接的方法，用频率特性仪，接换能器正负极测量读数 或者拿一片超声波振子接受对方发射的超声波进行放大后，输入到整形电路，再输入到计数器，就可以测量频率了
频率，是单位时间内完成周期性变化的次数，是描述周期运动频繁程度的量，常用符号f或ν表示，单位为秒分之一，符号为s。为了纪念德国物理学家赫兹的贡献，人们把频率的单位命名为赫兹，简称"赫"，符号为Hz。每个物体都有由它本身性质决定的与振幅无关的频率，叫做固有频率。频率概念不仅在力学、声学中应用，在电磁学、光学与无线电技术中也常使用。
能不能用kappa检验，我看了论坛上的帖子，好像是不行的。但是看到一篇文章，计数资料却用了kappa检验，请问能否这样使用，如果能的话，应该用什么软件，怎么计算。谢谢
提到的文章见后
文章.rar(267.87k)
缺点是人工计数可能发生的随机误差较大。
1、同样的测量条件下，如测量相对几何位置相同，放射源相同，放射性测量计数率扣除本底后与活度在统计误差内成正比；
2、同样活度不同核素放射源，在其它条件相同的情况下放射性测量计数率不一定相同。因为不同核素衰变各能量分支比不同、粒子不同、能量不同，有的衰变子体也有放射性。
3、放射性测量计数率与计数器的探测效率相关。简单认为：活度＝（计数率-本底计数率）/效率。其中效率的标定要与测量条件一致。
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别告诉我计数的方法用数，我今天早上数了0.4g的微丸，近1000粒，快成熊猫，差点被送动物园了。也别告诉我测微丸的粒径用尺子量，我可是想着把它们排成一条线（增加准确度，可以避免忽略小粒子）再量，可是，这些小粒子不像《花园宝宝》里的小点点们那样听话，他们不爱排队。
各位老师是否还有什么高招？小的在这请教了。
