| Product Name | Biotin - ACTH (1 - 39), humanBiotin - SYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEF |
| Size | 0.5 mg |
| Catalog # | AS-23968 |
| US$ | $232 |
| Purity | % Peak Area By HPLC ≥ 95% |
This C-terminally labeled biotin ACTH (1-39) has been used in ELISA assays. Adrenocorticotropic hormone (ACTH), also known as corticotropin, is a cleavage product from a larger precursor proopiomelanocortin (POMC). This 39 amino acid-peptide hormone is produced in the anterior pituitary gland upon stimulation by the corticotropin releasing hormone from the hypothalamus in response to stress. It stimulates the secretion of steroid hormone, specifically glucocorticoids in the adrenal cortex by acting through a cell membrane receptor (ACTH-R). In mammals, the action of ACTH is limited to those areas of the adrenal cortex in which the glucocorticoid hormones cortisol (hydrocortisone) and corticosterone are formed. ACTH has little control over the secretion of aldosterone, the other major steroid hormone from the adrenal cortex. | |
| Detailed Information | |
| Storage | -20°C |
| References | Moreno-Guzmán, M. et al. Biosensors Bioelectronics 35, 82 (2012). doi: 10.1016/j.bios.2012.02.015Stewart, PM. et al. Clin Endocrinol 40, 199 (1994)Elias, LL. and AJ. Clark, Braz J Med Biol Res 33, 1245 (2000)Latronico, AC. Braz J Med Biol Res 33, 1249 (2000) |
| Molecular Weight | 4767.6 |
| Biotin-SYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEF | |
| Sequence(Three-Letter Code) | Biotin - Ser - Tyr - Ser - Met - Glu - His - Phe - Arg - Trp - Gly - Lys - Pro - Val - Gly - Lys - Lys - Arg - Arg - Pro - Val - Lys - Val - Tyr - Pro - Asn - Gly - Ala - Glu - Asp - Glu - Ser - Ala - Glu - Ala - Phe - Pro - Leu - Glu - Phe - OH |
| Product Citations | Moreno-Guzmán, M. et al. (2012). Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensors. Biosensors Bioelectronics 35, 82. doi: 10.1016/j.bios.2012.02.015. |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
问:1如才能提高柱子吸附蛋白量?
2样品中的核酸对吸附有无太大影响?
3我拟采用阴离子交换树脂,问pH8.0条件下除去核酸可否?
4怎么提高样品的澄清度?
另外还想问一下,还有其他可行的破碎方法吗
谢谢
我怀疑溶菌酶有问题,因为其消化的30min内菌液几乎没大变化,而我曾看到别人用溶菌酶消化时菌液变的挺粘稠。
今下午再做时还是没什么变化,但发现溶菌酶在冰上静置2-3h后竟然沉淀下来了,而且上清和下面的白色溶菌酶分界明显,是我的溶菌酶配的有问题吗?出现这种情况溶菌酶还能用吗?我是-20度保存的。
下一步怎么处理菌液呢?
请高手多多指教!
大家请赐教!

