Product Description
TC-Protector is a chemically defined cell cryo-protective agent that does not contain any animal-derived components or proteins. It allows direct freezing of variety of cell lines at -80°C. Cells cryo-preserved with TC-protector have shown a high cell viability and durability rate on thawing.It can be used in cells requiring serum addition, cells without serum, and cells without proteins. It is suitable for both cell line and normal cells, as well as ES cells, adult stem cells, and human iPS cells. It is not viscous and does not form bubbles, making it excellent for use in these contexts.
Pack Size: 100 mL x 1 or 10 mL x 10
Physical Appearance: Clear liquid
Storage: Protect from light and storage at 4°C, avoid freezing prior to use
Characteristics:- Free of animal-derived components- Does not contain any serum or protein- Low viscosity and easy to use- Can be stored in a refrigerator and used immediately- Does not require special equipment to freeze cells
Key Application: Cryo-preserve cells directly without using programmed freezer at -80°C.Instructions for UseFreezing Method1. Cell Preparation:- For adherent cells, media should be changed at 80% confluence one day before cryopreservation. For the suspension cultured cells, change or supplement media one day before the cryopreservation.- It is important to preserve cells during the logarithmic growth phase for the best result.- Cryo-preserving confluent or overgrown cells will result in a decreased viability rate on thawing.2. Harvest the cells by centrifuging cells in medium at 1,500 rpm for 1 minute.3. Remove supernatant and re-suspend cells in TC-Protector at a concentration of between 5x106 and 1x107 cells/mL. Notes: Cell suspension should be kept on ice while preparing an appropriate (adequate) cell density.Appropriate cell number varies depending on the type of cell. Preliminary studiesare required for determining the optimum cell density. In general, 1×106 cells/mL is a recommended starting density.4. Freeze the cells in a deep freezer at -80 ºC. Transfer to liquid nitrogen if preferred on the following day.Thawing Method1. Thaw frozen vials rapidly in a warm water bath at 37ºC until it becomes a small ice mass.2. Transfer the cell suspension to a conical centrifuge tube and dilute by adding about x10 media. Centrifuge at 1,000～1,500 rpm for 1～2 minutes.3. Discard the supernatant after verifying cell pellet at the bottom of the tube.4. Seed cells in accordance with the standard method.
References:Miyagi-Shiohira, C. et al. Evaluation of Serum-Free, Xeno-Free Cryopreservation Solutions for Human Adipose-Derived Mesenchymal Stem Cells. Cell Med 9, 15–20 (2016).https://doi.org/10.3727/215517916X693122Takebe, T. et al. Transient vascularization of transplanted human adult-derived progenitors promotes self-organizing cartilage. J Clin Invest 124, 4325–4334 (2014).https://doi.org/10.1172/JCI76443Miwa, H., Hashimoto, Y., Tensho, K., Wakitani, S. & Takagi, M. Xeno-free proliferation of human bone marrow mesenchymal stem cells. Cytotechnology 64, 301–308 (2012).https://doi.org/10.1007/s10616-011-9400-7
FAQ:Q: How long can I keep cells at - 80ºC with specially designed freezing media?A: KAC recommends not to exceed a few months in a deep freezer, due to the temperature fluctuation caused by opening and closing the freezer door. If you want to cryopreserve for more than one month, using liquid nitrogen is recommended. Cells can be preserved indefinitely by storing in liquid nitrogen.Q: Does TC-Protector contain calcium and magnesium?A: Yes. However, the actual concentration is proprietary information.Q: What is the composition of TC-Protector?A: It is proprietary information. We can disclose only if we have a non-disclosure agreement (NDA).Q: Does TC-Protector contain any components of animal origin?A: There are no components of animal origin.Q: What is the ratio to mix TC-Protector and media when cryopreserving cells?A: TC-Protector is provided in a ready-to-use form. Do not mix with media. Simply suspend cells in TC-protector, verify the cells are not aggregated then place the vial in deep freezer.
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Na、K离子的通透性直接受膜电位的调节，如果指定某一电位(膜片钳钳制为某一电位)时，跨膜离子通透性一定，当然此时是可以通过测定电流来算出电导性， 其应该是某一不变的值；而膜片钳测定电导性的变化是怎么实现的？
我的上述思维里，肯定是有某个地方出现错误，苦于一直想不明白，求指点，谢谢！
电压等于电位差。参考点不同则各点电位值不同，但是各点之间的电压不变。向左转|向右转如图，a 是参考点，电位 Va = 0 ，Vb = E1 ，Ve = - E2 。电压 U1 = Vb - Vc ，U2 = Vc - Vd 。
假如A点的电位是10V,B点的电位是8V,C点的电位是6V.那么A到B之间的电压差是2V,A到C之间的电压是4V.
通俗点讲:我们把两节干电池串联,接个小灯泡,小灯泡两端的电压差就是3V,而一节干电池的两端的电压差是1.5V.如果我们将两节干电池并联,再接个小灯泡,小灯泡两端的电压差就是1.5V(并接电池的作用是加大容量,这个估计你还不懂)
电压差就是用高电位减去低电位的差值.设干电池的正极为1.5V,负极为0V,两点的电压差就是1.5V.设干电池的正极为0V,负极就为-1.5V.当两节干电池串联的时候,一节的负极与另外一节的正极连在一起,他们就是等电位,都是0V,所以第一节的正极1.5V减去第二节的负极-1.5V就得出从第一节的正极,到第二节的负极的电压差为3V.
更通俗的说:电位就相当于你现在所在的楼层,比如你现在在5楼,另外有个电位是在二楼,你和他就相差3层楼(这就是电压差),如果另外一个电位在地下1层,那你和他就相差6层(这个也是电压差)
我是苏州大学心血管内科研究生，我们打算使用电压敏感染料膜电位光学标测技术做体外心肌细胞的实验。但是目前实验室没人做过，想上门拜师学习！谢谢！~~~
我的联系方式：15962175904姓名：林欢单位：苏州大学医学院
例如，我们认为大地的电位为0，这就是参考点，现在有A、B两点，A点对地的电位是＋5V，B点对地的电位是－3V。我们可以说，A与地之间的电压是5V，B与地之间的电压是3V，但A与B之间的“电位差”是8V，因此AB两点之间的电压是8V。
