
Product Name | AnaTagᵀᴹHiLyteᵀᴹFluor 647 Protein Labeling Kit *Ultra Convenient* [3 labeling reactions (3 x 5 mg protein)] |
Size | 1 kit |
Catalog # | AS-72049 |
US$ | $374 |
HiLyte Fluor 647 acid, SE (Ex/Em=647 nm/679 nm) is an excellent fluorescent labeling dye that generates the protein conjugates that are slightly red-shifted compared to those of Cy® 5 dyes, resulting in an optimal match to filters designed for Cy®5 dyes. However, total fluorescence of the secondary antibody conjugates of HiLyte Fluor 647 dyes is significantly higher than that of Cy®5 conjugates supplied by other companies. Also, unlike Cy®5 dyes, HiLyte Fluor 647 dyes have very little change in absorption or fluorescence spectra when conjugated to most proteins, oligonucleotides and nucleic acids, thus yielding greater total fluorescence at the same degree of substitution. The HiLyte Fluor 647-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. Additionally, our in-house data indicated that HiLyte Fluor 647 is superior to Cy5 in fluorescence polarization-based assays. The kit has all essential components for performing the conjugation reaction and for purifying the HiLyte Fluor 647-protein conjugate. The entire process takes only half an hour. The kit is able to perform three conjugation reactions with each reaction labeling up to 5 mg IgG. Kit Size: 3 labeling reactions (3 x 5 mg protein) | |
Detailed Information | ![]() ![]() |
Product Citations | do Nascimento, ASF. et al. (2015). Algal lectin binding to core (α16) fucosylated N-glycans: Structural basis for specificity and production of recombinant protein. Glycobiol 25, 607. doi: 10.1093/glycob/cwv002. |
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【杂志】lifescience7020021185-1203
【作者】Mei-HanHuang,Sheng-NanWu,Chi-PienChen,Ai-YuShen
【单位】DepartmentofPharmaceuticalScience,FooYinInstituteofTechnology,Ta-Liao,KaohsiungCounty,Taiwan,R.O.C.DepartmentofMedicalEducationandResearch,KaohsiungVeteransGeneralHospital,KaohsiungCity,TaiwanDepartmentofPharmacy,TajenInstituteofTechnology,Pingtung,Taiwan,R.O.C.
【文摘】Quinoneshavebeenshowntopossessantineoplasticactivity;however,theireffectsonioniccurrentsremainunclear.Theeffectsof2-mercaptophenyl-1,4-naphthoquinone(2-MPNQ),menADIone(MD)and1,4-naphthoquinone(1,4NQ)oncellproliferationandioniccurrentsinpituitaryGH3lactotrophswereinvestigatedinthisstudy.2-MPNQwasmorepotentthanmenadioneor1,4-naphthoquinoneininhibitingthegrowthofGH3cells.2-MPNQdecreasedcellproliferationinaconcentration-dependentmannerwithanIC50valueof3mM.Inwhole-cellrecordingexperiments,2-MPNQreversIBLycausedaninhibitionofCa21-activatedK+current(IK(Ca))inaconcentrationdependentmanner.TheIC50valuefor2-MPNQ-inducedinhibitionofIK(Ca)was7mM.Intheinsideoutconfigurationofsinglechannelrecording,2-MPNQ(30mM)appliedintracellularlysuppressedtheactivityoflarge-conductanceCa21-activatedK+(BKCa)channelsbutdidnotmodifysinglechannelconductance.Menadione(30mM)hadnoeffectonthechannelactivity,whereas1,4-naphthoquinone(30mM)suppresseditbyabout26%.Both2-MPNQandthimerosalsuppressedthedithiothreitol-stimulatedchannelactivity.2-MPNQalsoblockedvoltage-dependentK+currents,butitproducedaslightreductionofL-typeCa21inwardcurrent.However,unlikeE-4031,2-MPNQ(30mM)didnotsuppressinwardlyrectifyingK+currentpresentinGH3cells.Underthecurrentclampconfiguration,thepresenceof2-MPNQ(30mM)depolarizedthecells,andincreasedthefrequencyanddurationofspontaneousactionpotentials.The2-MPNQ-mediatedinhibitionofK+currentswouldaffecthormonesecretionandcellexcitABIlity.Theblockadeoftheseionicchannelsby2-MPNQmaypartlyexplainitsinhibitoryeffectontheproliferationofGH3cells.
【翻译】醌类化合物已经发现具有抗肿瘤的作用,然而它对于离子通道的影响还不是很清楚。此研究的内容是MPNQ和1,4NO的对于垂体瘤GH3细胞的增殖和离子通道方面的影响。2-MPNQ对于GH3细胞的生长抑制作用较甲萘醌和1,4NO更强。2-MPNQ以3uM的浓度能降低细胞增殖的浓度依赖IC50.在全细胞记录的实验中,MPNQ可逆性的引起I(Kca)浓度依赖的抑制。IC50的MPNQ抑制I(Kca)的浓度是7uM。在单一通道的记录中,2-MPNQ(30mM)可以抑制细胞内BKCa的传导,但并不影响单通道的传导。甲萘醌(30mM)对于此通道没有影响,1,4NO在此浓度下的抑制率大约为26%。2-MPNQ和硫柳汞可以抑制二硫苏糖醇刺激性通道的活动。2-MPNQ也可以阻滞电压依赖的钾通道,但对于L型钙通道的内流有轻微的抑制作用。2-MPNQ与E4031不同的是它并不抑制GH3细胞的内流的钾电流。在当前的研究下表明,2-MPNQ可以使GH3细胞增殖,并且能够增加自发动作电位的频率和持续时间。2-MPNQ介导的钾电流的抑制可能会影响到激素的分泌和细胞兴奋性。2-MPNQ对于这些离子通道的阻滞作用可以部分解释它是如何对GH3细胞增殖的抑制作用。
【点评】比较标准的电生理的SCI文章
例如,我们认为大地的电位为0,这就是参考点,现在有A、B两点,A点对地的电位是+5V,B点对地的电位是-3V。我们可以说,A与地之间的电压是5V,B与地之间的电压是3V,但A与B之间的“电位差”是8V,因此AB两点之间的电压是8V。
我是苏州大学心血管内科研究生,我们打算使用电压敏感染料膜电位光学标测技术做体外心肌细胞的实验。但是目前实验室没人做过,想上门拜师学习!谢谢!~~~
我的联系方式:15962175904姓名:林欢单位:苏州大学医学院
问题1,钠通道的大量激活,是只有在达到阈刺激时才能大量激活还是达到一个特定电位(不管骨骼肌还是心肌这个特定电位不变)才能激活?
问题2,激活是一个瞬态,瞬间大量激活,瞬间大量失活,那这个动作电位上升支持续的时间怎么解释?
问题3,钠通道的失活应该也是电压依赖性的,导致失活电压是特定的么?在骨骼肌和心肌一样么?
问题4,在从静息电位到阈电位这一段时间,第106页心肌的图和第39页骨骼肌的图一个兴奋性一个不变一个兴奋性增大怎么解释?
问题5,在第106页的第2个图上在RRP和SNP时期引出动作电位,动作电位的上升支不是应该接近钠离子的平衡电位才终止么,而这个平衡电位应该只跟细胞内外钠离子的浓度变化有关,但动作电位导致的细胞内外钠离子的浓度变化应该不大,怎么会使出现几个动作电位的上升的最高点是不一样的呢?
问题6,怎么理解钠离子通道的时间依赖性?33页的图怎么看?为什么随着电压增大,钠电导增大呢?不是只有在阈刺激时钠通道才大量开放,这个时候才应该钠电导最大么?
参考资料:http://baike.baidu.com/search/word?word=%E7%94%B5%E4%BD%8D
电压(voltage),也称作电势差或电位差,是衡量单位电荷在静电场中由于电势不同所产生的能量差的物理量。其大小等于单位正电荷因受电场力作用从A点移动到B点所做的功,电压的方向规定为从高电位指向低电位的方向。电压的国际单位制为伏特(V,简称伏),常用的单位还有毫伏(mV)、微伏(μV)、千伏(kV)等。此概念与水位高低所造成的“水压”相似。需要指出的是,“电压”一词一般只用于电路当中,“电势差”和“电位差”则普遍应用于一切电现象当中。
参考资料:http://baike.baidu.com/view/10954.htm
希望我的回答能够帮助到你,望采纳,谢谢。

