
TakaraPODconjugatesaredesignedtoreplacetrADItionalsecondaryantibodiesinimmunohistochemicalstainingofparaffin-embeddedtissuesections.Usetheseproductsforasimplerandeasiermethodthanthosebasedonstreptavidin-biotin,andobtainclear,highlysensitiveresultswithminimalbackground.EachTakaraPODconjugateconsistsofanaminoacidpolymercombinedwithperoxidaseandtheFab"fragmentsofasecondaryantibody.Thelabeledpolymersbindtotheprimaryantibody-antigentoformanHRP-labeledcomplex.Thecomplexisvisualizedbytheadditionofacolorimetricsubstrate,suchasDABSubstrate(seebelow).
TakaraPODconjugatesaredesignedtoreplacetraditionalsecondaryantibodiesinimmunohistochemicalstainingofparaffin-embeddedtissuesections.Usetheseproductsforasimplerandeasiermethodthanthosebasedonstreptavidin-biotin,andobtainclear,highlysensitiveresultswithminimalbackground.
EachTakaraPODconjugateconsistsofanaminoacidpolymercombinedwithperoxidaseandtheFab"fragmentsofasecondaryantibody.Thelabeledpolymersbindtotheprimaryantibody-antigentoformanHRP-labeledcomplex.Thecomplexisvisualizedbytheadditionofacolorimetricsubstrate.
Thisproductisavailableintwodifferentformats,formousetissuesections("ForMouseTissue")andfortissuesectionsfromothermammaliansources("ForTissue").Specificversionsareavailablebasedonthesourcespeciesoftheprimaryantibody;"AntiMouse","AntiRat",and"AntiRabbit"versionsareavailableformousetissuesections,and"AntiMouse","AntiRabbit",and"AntiGoat"versionsareavailableformammaliantissue.
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谢谢您的帮助!我是药学专业的,一直在做动物的药效试验,以前没有做过分子实验,忘各位指点:)
这样子好吗?我个人认为理论上问题不大,不知道是不是

