One-Step RT-PCR Kit [Ab+Taq]
- Fast and easy one-tube setup
- Convenient enzyme mix and 2x reaction mix
- Fast activation of PCR enzyme
- Heat-labile UDG system: prevention of carryover contamination
- Convenience and reduced contamination risk
- The high specific and sensitive PCR amplification
Products
Cat.No. | Product | Feature | Size |
---|---|---|---|
BORTA | One-Step RT-PCR Kit [Ab+Taq] | - | Custom |
BORTAU | One-Step RT-PCR Kit [Ab+Taq] , w/UDG | + UDG SYSTEM* | Custom |
- Description
- Data
- Specification
- Documents
One-Step RT-PCR Kit [Ab+Taq] is designed for specific and sensitive amplification of target genes in one-tube reaction from RNA templates. Thermo Reverse Transcriptase, Ab+Taq polymerase, and RNase Inhibitor are supplied as an enzyme mixture. An optimized buffer is provided as a 2x Reaction mix containing a buffer, MgCl2, and dNTPs as 2x concentration. Applied UDG system includes the Heal-labile UDG and dUTP.
- Viral RNA detection
- Gene-expression analysis
- Molecular Diagnosis
- Endpoint one-step RT-PCR from any RNA transcript
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实验需要请问有没有养THP-1细胞的同仁?能否寄一皿我,我在广州南方医科大学,不胜感激!
同学说传代必须是一皿传多皿,一皿传一皿就不算传代~求高手指点~~
传代后5天,依然没有长满,怕影响活力,想传代和冻存,不知这样是否可以??
所谓上皿式指的是称量盘在支架上面;而称量盘吊挂在支架下面的为下皿式。
目前使用较为广泛的是上皿式电子天平。
想问下各位大神,本人用皿养的PC-9GR细胞突然有大片飘起,也并没有成团状飘起,求解决方法?
它最初由在德国生物学家罗伯特·科赫手下工作的细菌学家朱利斯·理查德·佩特里(1852-1921)于1887年设计,故又称为“佩特里皿”。
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其实就是常见的圆形的上下盖培养皿,你可以看看它的英文,就叫做petri dish,直译过来就叫做——佩特里小碟子——也就是皮氏培养皿
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第二,三段为原创手打……第一段为摘抄
如题~
已经基本养好了贴壁的原代细胞了,准备鉴定了,看到好多都在说细胞爬片的问题,我可以不做爬片直接在皿里操作吗?具体需要注意些什么呢?感谢各位大神
激光共聚焦的看活细胞皿哪里买呢