Dog CRP ELISA Kit
CATALOG # E-40CRP- Add to Wishlist
Details
- Canine, Dog
- Chicken
- ELISA
- CRP
- 2-8C
- Plasma, Serum, Urine
- 3.125 ng/ml - 200 ng/ml
- 0.855 ng/ml
- 25 min.
- The principle of the double antibody sandwich Dog/Canine CRP ELISA is represented in Figure 1. In this assay the CRP present in samples reacts with the anti-Dog CRP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Dog CRP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of canine CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Documents
- Data Sheet
- SDS
Kit Contents
- 1
One ELISA Micro Plate with 12 removable (8 well) micro well strips in holding frame, each coated with Affinity Purified Antibody
- 2
One ELISA Kit Data Sheet
- 3
One Certificate of Analysis
- 4
One 50 mL bottle of Diluent Running Buffer
- 5
One 50 mL bottle of 20X Concentrated Wash Solution
- 6
One 150 uL Vial of Affinity Purified HRP Conjugated Antibody in stabilizing buffer
- 7
One 12 mL vial of Chromogen-Substrate Solution
- 8
One 12 mL vial of Stop Solution
- 9
One Calibrator Vial
Citations
This product is for research use only, not for diagnostic or therapeutic use.
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抗原注射进入动物体内后,因为不是一个单独的分子,会随血液循环输送到各处,也就会接触不同的免疫细胞。有些还会被抗原提呈细胞再加工。所以最后的结果是很多个免疫细胞都会接触到。一个抗原分子也有很多抗原表位。每个表位理论上都有能力激活一个B细胞转化为浆细胞。所以体内最后针对该抗原的必定是多克隆
由于动物个体差异的存在,同一抗原免疫同一种系不同个体的动物,产生的抗体的效价有很大的差异.与动物的年龄和营养状况密切相关.免疫用的动物最好选择适龄的健康雄性动物,雌性动物特别是妊娠动物用于制备免疫抗体则非常不合适,有时甚至不产生抗体.
先饲养观察7天其实就是等于隔离检疫,观察一下兔子的身体状况和是否带有其他传染病,淘汰体质多病的个体,防止在免疫过程中死亡或免疫应答不灵敏而不产生抗体.
IgM占血清免疫球蛋白总量的6%,主要存在血管内,是机体受抗原刺激后最先产生的抗体,起“先锋免疫”作用,具有很强的细胞毒活性和细胞溶解活性,由于IgM主要存在在血管内,是抗血管内感染的第一线抗体,对防止败血症的发生有重要作用。
IgG-主力抗体
IgG是初级免疫应答中最持久、最重要的抗体,它仅以单体形式存在。大多是抗菌性、抗毒性和抗病毒抗体属于IgG,它在抗感染中起到主力军作用,它能够促进单核巨噬细胞的吞噬作用(调理作用),中和细菌毒素的毒性(中和毒素)和病毒抗原结合使病毒失去感染宿主细胞的能力(中和病毒)。
IgA-局部抗体
按其免疫功能又分为血清型及分泌型两种。血清型IgA存在于血清中,其含量占总IgA的85%左右。血清型IgA虽有IgG和IgM的某些功能,但在血清中并不显示重要的免疫功能。分泌型IgA存在于分泌液中,如唾液、泪液、初乳、鼻和支气管分泌液、胃肠液、尿液、汗液等。分泌型IgA是机体粘膜局部抗感染免疫的主要抗体。故又称粘膜局部抗体。

