Catalogue number
HIV-101
Introduction
Human immunodeficiency virus (HIV) is a retrovirusthat can lead to a condition in which the immune systembegins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the humanimmune systemsuch as helper T cells(specifically CD4+ T cells), macrophagesand dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: firstly, direct viral killing of infected cells; secondly, increased rates of apoptosisin infected cells; and thirdly, killing of infected CD4+ T cells by CD8 cytotoxic lymphocytesthat recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunityis lost, and the body becomes progressively more susceptible to opportunistic infections. HIV was classified as a member of the genus Lentivirus, part of the family of Retroviridae. Lentiviruses have many common morphologies and biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period. Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genomeis converted to double-stranded DNAby a virally encoded reverse transcriptasethat is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integraseso that the genome can be transcribed. Once the virus has infected the cell, two pathways are possible: either the virus becomes latentand the infected cell continues to function, or the virus becomes active and replicates, and a large number of virus particles are liberated that can then infect other cells.
Description
HIV-1 envelope is an E.coli-derived recombinant protein that composes all of the reported immunogenic determinants found in gp41 and a small portion of gp120. The gene encoding this fusion protein was synthesized using codons optimized for E.coli expression and doesn’t represent a linear HIV-1 envelope sequence. HIV-1 is a non-glycosylated, 233 amino acid polypeptide chain, having a molecular mass of 27275.88 dalton and pI=9.68. HIV-1 envelope protein spans the C-Terminus of gp120 and most of gp41. Superior diagnostic reagent for HIV-1 and HIV type-O detection. Detects all HIV-1 and HIV-type O infected individuals responding to envelope proteins.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless clear solution.
Formulation
The HIV-1 contains 0.5X PBS & 6M urea.
Purity
Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.
Stability
HIV-1 Envelope although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Specificity
Immunoreactive with all sera of HIV-1 and HIV-type O infected individuals and with 60-80% of HIV-2 infected individuals.
Applications
HIV-1 Envelope antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
Safety Data Sheet
Usage
ProSpec"s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。

ProSpec-TanyTechnoGeneLtd.公司创立人Dr.Tanchum(Tany)Amarant主要致力于蛋白生物化学方面的研究工作,1947年出生于以色列。1991-1997创建ReProGen公司,从事细胞因子和趋化因子的大量生产及纯化;1997-2000创建ProSpec-TechnoGene,主要生产用于生命科学研究的各种重组蛋白及相关抗体。拥有专利UnitedStatesPatent5434135。
ProSpec的生产和研发工厂位于以色列雷霍沃特(Rehovot),专注于蛋白(重组及合成)生产研发。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。

欢迎各位ProSpec客户及合作伙伴拨打全国免费电话400-6800-868转801/802/803垂询蚂蚁淘科技问询相关产品及解决方案!
代理ProSpec细胞因子、生长因子、激素、病毒抗原等重组蛋白产品简介
为便于客户选购ProSpec公司的各种重组蛋白,特将选购及使用注意事项列举如下:
1、需要考虑细胞因子、生长因子的种属来源和表达细胞选择
ProSpec可以提供多种不同种属来源的细胞因子等重组蛋白,如白介素-3,可以提供人白介素-3、小鼠白介素-3、大鼠白介素-3;而瘦蛋白Leptin则有人源、小鼠源、大鼠源、绵羊源、猪源、河豚源、马源、鸡源、狗源、兔源等十多种不同Leptin。蚂蚁淘科技推荐,建议按照作用的细胞选择相同种属的细胞因子。不过不同种属之间,蛋白同源性(序列一致性)在80%以上的基本上就可确定为有交叉活性,可推荐使用。另外,蚂蚁淘还可以向您提供ProSpec不同种属细胞表达的细胞因子,以重组人血管内皮生长因子(rVEGF)为例,ProSpec可以提供大肠杆菌表达的、酵母Yeast表达的、中国仓鼠卵巢CHO细胞、人胚肾HEK细胞表达的VEGF。可以根据您的实验需求,垂询蚂蚁淘科技有限公司为您推荐不同细胞表达的细胞因子产品。
2、ProSpec细胞因子的溶解和保存建议
ProSpec绝大部分细胞因子产品是天然成熟型蛋白,而不含有载体蛋白(CarrierProtein)或其他添加剂(如BSA、HAS或蔗糖等)。同时,ProSpec-Tany绝大部分产品通常以最少量的盐来进行冻干处理,因此易于运输和保存。所以我们建议在收到产品后,务必在开盖前先离心,使粘在管盖或管壁上的蛋白聚集于管底(此时能否见到白色沉淀均属正常现象)。为获得更好生物活性和保存稳定性,请联系蚂蚁淘科技垂询相关的溶解以保存建议。
领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司,而小包装形式的细胞因子也受广大研究生客户喜爱。作为ProSpec的中国总代理,蚂蚁淘科技有限公司为客户精选真核细胞表达、高活性的几十多种常用现货ProSpec细胞因子。更多细胞因子产品请查询蚂蚁淘网站或垂询蚂蚁淘科技有限公司。

ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。