These mammalian expression vectors allow you to express a protein of interest fused to either the c-Myc or hemagglutinin (HA) epitope tag. c-Myc and HA are well-characterized and highly immunoreactive tags, and thus are ideal for co-IP studies but are also easily detected via Western blot. Moreover, c-Myc and HA are small tags and thus unlikely to interfere with the function of your protein.
These mammalian expression vectors allow you to express a protein of interest fused to either the c-Myc or hemagglutinin (HA) epitope tag. c-Myc and HA are well-characterized and highly immunoreactive tags, and thus are ideal for co-IP studies but are also easily detected via Western blot. Moreover, c-Myc and HA are small tags and thus unlikely to interfere with the function of your protein.
The myc- or HA-tagged protein of interest can be detected by either western analysis or fluorescence microscopy using an anti-myc antibody or anti-HA antibody.
The pCMV-Myc & pCMV-HA Vector Set includes c-Myc Monoclonal and HA-Tag Polyclonal Antibodies, which can also be used in Western blot, ELISA, or immunostaining assays. A sequencing primer is also included in the set to confirm that the insert is in the correct reading frame.
HA tag sequence: ACC ATG TAC CCA TAC GAT GTT CCA GAT TAC GCT
Myc tag sequence: ATG GCA TCA ATG CAG AAG CTG ATC TCA GAG GAG GAC CTG
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所以一个细菌或病毒可以有很多的抗原。
若已进入细胞内。则先由巨噬细胞吞噬降解。并形成抗原-MHC复合体传送到表面。被辅助性T淋巴细胞识别和效应T淋巴细胞识别后。效应T淋巴细胞被激活。分解靶细胞。然后再由效应B细胞分泌的抗体进一步消灭病毒。
还有不清楚的么？
荚膜（capsule）是某些细菌在生长繁殖过程中分泌的一层黏液性物质，包围在细胞壁外，通常这种黏液层厚度小于0.2μm，成分是多糖或多肽，只有在营养丰富时或在动物体内，细菌才产生这种半抗原性质的黏液性物质。它具有保护菌体免受巨噬细胞等的捕捉和吞噬，因而具有抗吞噬抗消化、侵袭力强、与致病性关系密切等特点。像肺炎球菌、炭疽杆菌等都有这类荚膜。有些细菌的荚膜层较薄，小于0.2μm，称为微荚。
像链球菌的M蛋白、伤寒杆菌的Vi抗原、大肠杆菌的K抗原等都属于这类微荚膜。
请赐教！
