PRODUCT SPECIFICATIONS
ITEM# 5009: Recombinant SIV gp41 Envelope Protein (E.coli)
SPECIFICATIONS
Concentration: See vial
Mass/vial: 0.1mg
Volume/vial: see vial
Diluent: 50 mM Tris, 0.1M NaCl, 2mM DDT, pH8
Purity: >98%
Stabilizer: None
Preservative: None
Storage: -75oC
Physical State: Frozen Liquid
Stability: At least 24 months at -75 oC.
DESCRIPTION: Truncated Recombinant SIV gp41 envelope protein produced in the E.coli Expression System. Truncated gp41 protein mol.wt: 28kD.
Applications : In- Vitro Diagnostics,Immunization, Elisa/Western Blots.
PURIFICATION: This protein was purified by solvent extraction and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.
SPECIFICITY: This protein binds to HIV-2 converted human serum polyclonal antibodies in ELISA and Western Elisa.
BIOLOGICAL ACTIVITY: Not done
APPLICATION AND INSTRUCTIONS FOR USE
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.
Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard
ELISA conditions.
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请赐教！
荚膜（capsule）是某些细菌在生长繁殖过程中分泌的一层黏液性物质，包围在细胞壁外，通常这种黏液层厚度小于0.2μm，成分是多糖或多肽，只有在营养丰富时或在动物体内，细菌才产生这种半抗原性质的黏液性物质。它具有保护菌体免受巨噬细胞等的捕捉和吞噬，因而具有抗吞噬抗消化、侵袭力强、与致病性关系密切等特点。像肺炎球菌、炭疽杆菌等都有这类荚膜。有些细菌的荚膜层较薄，小于0.2μm，称为微荚。
像链球菌的M蛋白、伤寒杆菌的Vi抗原、大肠杆菌的K抗原等都属于这类微荚膜。
颗粒型抗原,除了有细菌、红细胞、螺旋体等天然颗粒型抗原,还有吸附有可溶性抗原的非免疫相关颗粒.
颗粒性抗原光镜下可见，比如细菌性抗原、红细胞抗原等；而可溶性抗原在光镜下不可见，如组织浸出液、细菌毒素、蛋白质分子等。
它们不等同于完全抗原和不完全抗原。完全抗原具有免疫原性和抗原性，而不完全抗原只具有抗原性。完全抗原可以是颗粒性抗原，亦可是可溶性抗原，而不完全抗原一般只能是可溶性抗原，不会是颗粒性抗原。
若已进入细胞内。则先由巨噬细胞吞噬降解。并形成抗原-MHC复合体传送到表面。被辅助性T淋巴细胞识别和效应T淋巴细胞识别后。效应T淋巴细胞被激活。分解靶细胞。然后再由效应B细胞分泌的抗体进一步消灭病毒。
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