Product Specifications:
Item# 105-20: Recombinant M Tuberculosis CFP-10 Antigen (E.coli)
Concentration: 2mg/ml
Mass/vial: 1mg/vial
Volume/vial: 500ul
Diluent: 50mM NaPO4, pH 7.0 0.1M NaCl , 0.05% SOD.
Purity: >98%
Stabilizer: None
Preservative: None
Storage: -75°C
Physical State: Frozen Liquid
Stability: Not Determined
Description: M. Tuberculosis (virulent strain specific) CFP-10 antigen produced in the E. coli expression system.
Purificatiuon: Purified by ion affinity, solvent extraction and UF concentrated to >98% purity as determined by SDS-PAGE, reduced.
Molecular Weight: approx. 11kD
Specificity: This CFP-10 protein reacts with human TB serum in ELISA and Western ELISA. Suitable for diagnostic applications.
Biological Activity: Not Determined
Application and Instructions for use:
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. CAUTION: This product is sterile filtered and is stable at refrigeration temperature. Remove product aseptically from the vial.
Articles related to CFP-10:
Upon infection with Mycobacterium tuberculosis, neutrophils are massively recruited to the lungs, but the role of these cells in combating the infection is poorly understood. Through a type VII secretion system, M. tuberculosis releases a heterodimeric protein complex, containing a 6-kDa early secreted antigenic target (ESAT-6) and a 10-kDa culture filtrate protein (CFP-10), that is essential for virulence. Whereas the ESAT-6 component possesses multiple virulence-related activities, no direct biological activity of CFP-10 has been shown, and CFP-10 has been described as a chaperone protein for ESAT-6. We here show that the ESAT-6:CFP-10 complex induces a transient release of Ca2+ from intracellular stores in human neutrophils. Surprisingly, CFP-10 rather than ESAT-6 was responsible for triggering the Ca2+ response, in a pertussis toxin-sensitive manner, suggesting the involvement of a G-protein-coupled receptor. In line with this, the response was accompanied by neutrophil chemotaxis and activation of the superoxide-producing NADPH-oxidase. Neutrophils were unique among leukocytes in responding to CFP-10, as monocytes and lymphocytes failed to produce a Ca2+ signal upon stimulation with the M. tuberculosis protein. Hence, CFP-10 may contribute specifically to neutrophil recruitment and activation during M. tuberculosis infection, representing a novel biological role for CFP-10 in the ESAT-6:CFP-10 complex, beyond the previously described chaperone function.
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所以一个细菌或病毒可以有很多的抗原。
荚膜是某些细菌在细胞壁外包围的一层粘液性物质，一般由糖和多肽组成，是细菌的一种特殊结构。
作用：
①抗吞噬作用：荚膜因其亲水性及其空间占位、屏障作用，可有效抵抗寄主吞噬细胞的吞噬作用。
②黏附作用：荚膜多糖可使细菌彼此间粘连，也可黏附于组织细胞或无生命物体表面，是引起感染的重要因素，具有荚膜的S-型肺炎链球菌毒力强，有助于肺炎链球菌侵染人体；废水生物处理中的细菌荚膜有生物吸附作用，将废水中的有机物、无机物及胶体吸附在细菌体表面上。
③抗有害物质的损伤作用：处于细菌细胞最外层，荚膜犹如盔甲可有效保护菌体免受或少受多种杀菌、抑菌物质的损伤，如溶菌酶、补体等。
④抗干燥作用：荚膜多糖为高度水合分子，含水量在95%以上，可帮助细菌抵抗干燥对生存的威胁。
⑤当缺乏营养时，荚膜可被利用作碳源和能源，有的荚膜还可作氮源。
颗粒型抗原,除了有细菌、红细胞、螺旋体等天然颗粒型抗原,还有吸附有可溶性抗原的非免疫相关颗粒.
颗粒性抗原光镜下可见，比如细菌性抗原、红细胞抗原等；而可溶性抗原在光镜下不可见，如组织浸出液、细菌毒素、蛋白质分子等。
它们不等同于完全抗原和不完全抗原。完全抗原具有免疫原性和抗原性，而不完全抗原只具有抗原性。完全抗原可以是颗粒性抗原，亦可是可溶性抗原，而不完全抗原一般只能是可溶性抗原，不会是颗粒性抗原。
若已进入细胞内。则先由巨噬细胞吞噬降解。并形成抗原-MHC复合体传送到表面。被辅助性T淋巴细胞识别和效应T淋巴细胞识别后。效应T淋巴细胞被激活。分解靶细胞。然后再由效应B细胞分泌的抗体进一步消灭病毒。
还有不清楚的么？
