![NanoHelix/Direct PCR [3G]/200 rxns/DPRU200](images/NanoHelix/20190209121115686.jpg)
Direct PCR [3G]
- Amplify directly from whole blood, animal and plants
- Skip DNA purification
- Various genotyping
- Prevention of carryover contamination
Products
Cat.No. | Product | Size |
---|---|---|
DPR200 | HelixAmp™ Direct PCR [3G] | 200 rxns |
DPRU200 | HelixAmp™ Direct PCR [3G] [Containing UDG/dUTP] | 200 rxns |
DPRH200 | HelixAmp™ Direct PCR [3G] (Hot-start) | 200 rxns |
DPRHU200 | HelixAmp™ Direct PCR [3G] (Hot-start) [Containing UDG/dUTP] | 200 rxns |
- Description
- Data
- Details
- Documents
HelixAmp™ Direct PCR [3G] is designed for the PCR amplification directly from animal tissues, whole blood, and plant tissues without any DNA purification processes. This kit contains a 2x reaction mix including Taq DNA polymerase, dNTPs, MgCl2, and unique buffer system to resist various PCR inhibitors of tissue samples. HelixAmp™ Direct PCR [3G] (Hot-start) is including hot-start Taq DNA polymerase that is inactive at lower temperature by an inhibitory antibody and using this enzyme avoids the polymerization from non-specifically bound primers during the setting of PCR mix and at the start of PCR cycles. At high temperature of the initial denaturation step of PCR, the inhibitory antibody is released by denaturation and the free Taq DNA polymerase becomes active. NanoHelix’s Direct PCR [3G] is based on Taq polymerase. Due to this enzyme’s robust amplification and 3’ to 5’ exo-negative properties, this kit could be used for allele specific PCR which is routinely used for various genotyping. Moreover the Uracil-DNA glycosylase with dUTP system for the prevention of carryover contamination can be applied to this Taq polymerase-based kit. dUTP could not be used with the Pfu DNA polymerase and its derivatives. A Uracil-DNA glycosylase and dUTP applied version is also available.
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颗粒型抗原,除了有细菌、红细胞、螺旋体等天然颗粒型抗原,还有吸附有可溶性抗原的非免疫相关颗粒.
颗粒性抗原光镜下可见,比如细菌性抗原、红细胞抗原等;而可溶性抗原在光镜下不可见,如组织浸出液、细菌毒素、蛋白质分子等。
它们不等同于完全抗原和不完全抗原。完全抗原具有免疫原性和抗原性,而不完全抗原只具有抗原性。完全抗原可以是颗粒性抗原,亦可是可溶性抗原,而不完全抗原一般只能是可溶性抗原,不会是颗粒性抗原。
荚膜是某些细菌在细胞壁外包围的一层粘液性物质,一般由糖和多肽组成,是细菌的一种特殊结构。
作用:
①抗吞噬作用:荚膜因其亲水性及其空间占位、屏障作用,可有效抵抗寄主吞噬细胞的吞噬作用。
②黏附作用:荚膜多糖可使细菌彼此间粘连,也可黏附于组织细胞或无生命物体表面,是引起感染的重要因素,具有荚膜的S-型肺炎链球菌毒力强,有助于肺炎链球菌侵染人体;废水生物处理中的细菌荚膜有生物吸附作用,将废水中的有机物、无机物及胶体吸附在细菌体表面上。
③抗有害物质的损伤作用:处于细菌细胞最外层,荚膜犹如盔甲可有效保护菌体免受或少受多种杀菌、抑菌物质的损伤,如溶菌酶、补体等。
④抗干燥作用:荚膜多糖为高度水合分子,含水量在95%以上,可帮助细菌抵抗干燥对生存的威胁。
⑤当缺乏营养时,荚膜可被利用作碳源和能源,有的荚膜还可作氮源。
所以一个细菌或病毒可以有很多的抗原。

