![NanoHelix/Direct PCR [3G]/200 rxns/DPRU200](images/NanoHelix/20190209121115686.jpg)
Direct PCR [3G]
- Amplify directly from whole blood, animal and plants
- Skip DNA purification
- Various genotyping
- Prevention of carryover contamination
Products
| Cat.No. | Product | Size |
|---|---|---|
| DPR200 | HelixAmp™ Direct PCR [3G] | 200 rxns |
| DPRU200 | HelixAmp™ Direct PCR [3G] [Containing UDG/dUTP] | 200 rxns |
| DPRH200 | HelixAmp™ Direct PCR [3G] (Hot-start) | 200 rxns |
| DPRHU200 | HelixAmp™ Direct PCR [3G] (Hot-start) [Containing UDG/dUTP] | 200 rxns |
- Description
- Data
- Details
- Documents
HelixAmp™ Direct PCR [3G] is designed for the PCR amplification directly from animal tissues, whole blood, and plant tissues without any DNA purification processes. This kit contains a 2x reaction mix including Taq DNA polymerase, dNTPs, MgCl2, and unique buffer system to resist various PCR inhibitors of tissue samples. HelixAmp™ Direct PCR [3G] (Hot-start) is including hot-start Taq DNA polymerase that is inactive at lower temperature by an inhibitory antibody and using this enzyme avoids the polymerization from non-specifically bound primers during the setting of PCR mix and at the start of PCR cycles. At high temperature of the initial denaturation step of PCR, the inhibitory antibody is released by denaturation and the free Taq DNA polymerase becomes active. NanoHelix’s Direct PCR [3G] is based on Taq polymerase. Due to this enzyme’s robust amplification and 3’ to 5’ exo-negative properties, this kit could be used for allele specific PCR which is routinely used for various genotyping. Moreover the Uracil-DNA glycosylase with dUTP system for the prevention of carryover contamination can be applied to this Taq polymerase-based kit. dUTP could not be used with the Pfu DNA polymerase and its derivatives. A Uracil-DNA glycosylase and dUTP applied version is also available.
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请赐教！
荚膜（capsule）是某些细菌在生长繁殖过程中分泌的一层黏液性物质，包围在细胞壁外，通常这种黏液层厚度小于0.2μm，成分是多糖或多肽，只有在营养丰富时或在动物体内，细菌才产生这种半抗原性质的黏液性物质。它具有保护菌体免受巨噬细胞等的捕捉和吞噬，因而具有抗吞噬抗消化、侵袭力强、与致病性关系密切等特点。像肺炎球菌、炭疽杆菌等都有这类荚膜。有些细菌的荚膜层较薄，小于0.2μm，称为微荚。
像链球菌的M蛋白、伤寒杆菌的Vi抗原、大肠杆菌的K抗原等都属于这类微荚膜。
颗粒型抗原,除了有细菌、红细胞、螺旋体等天然颗粒型抗原,还有吸附有可溶性抗原的非免疫相关颗粒.
颗粒性抗原光镜下可见，比如细菌性抗原、红细胞抗原等；而可溶性抗原在光镜下不可见，如组织浸出液、细菌毒素、蛋白质分子等。
它们不等同于完全抗原和不完全抗原。完全抗原具有免疫原性和抗原性，而不完全抗原只具有抗原性。完全抗原可以是颗粒性抗原，亦可是可溶性抗原，而不完全抗原一般只能是可溶性抗原，不会是颗粒性抗原。
若已进入细胞内。则先由巨噬细胞吞噬降解。并形成抗原-MHC复合体传送到表面。被辅助性T淋巴细胞识别和效应T淋巴细胞识别后。效应T淋巴细胞被激活。分解靶细胞。然后再由效应B细胞分泌的抗体进一步消灭病毒。
还有不清楚的么？
