
About InVivoMAb anti-mouse CD8α
The YTS 169.4 monoclonal antibody reacts with mouse CD8α. The CD8 antigen is a transmembrane glycoprotein that acts as a co-receptor for the T cell receptor (TCR). Like the TCR, CD8 binds to class I MHC molecules displayed by antigen presenting cells (APC). CD8 is primarily expressed on the surface of cytotoxic T cells, but can also be found on thymocytes, natural killer cells, and some dendritic cell subsets. CD8 most commonly exists as a heterodimer composed of one CD8α and one CD8β chain however, it can also exist as a homodimer composed of two CD8α chains. Both the CD8α and CD8β chains share significant homology to immunoglobulin variable light chains. The molecular weight of each CD8 chain is approximately 34 kDa. The YTS 169.4 antibody exhibits depleting activity when used in vivo.
InVivoMAb anti-mouse CD8α Specifications
Isotype | Rat IgG2b, κ |
Recommended Isotype Control(s) | InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin(BE0090) |
Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer(IP0070) |
Immunogen | CBA mouse thymocytes |
Reported Applications |
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Endotoxin |
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Purity |
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Formulation |
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Sterility | 0.2 μM filtered |
Production | Purified from tissue culture supernatant in an animal free facility |
Purification | Protein G |
Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
RRID | AB_10950145 |
Molecular Weight | 150 kDa |
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颗粒型抗原,除了有细菌、红细胞、螺旋体等天然颗粒型抗原,还有吸附有可溶性抗原的非免疫相关颗粒.
颗粒性抗原光镜下可见,比如细菌性抗原、红细胞抗原等;而可溶性抗原在光镜下不可见,如组织浸出液、细菌毒素、蛋白质分子等。
它们不等同于完全抗原和不完全抗原。完全抗原具有免疫原性和抗原性,而不完全抗原只具有抗原性。完全抗原可以是颗粒性抗原,亦可是可溶性抗原,而不完全抗原一般只能是可溶性抗原,不会是颗粒性抗原。
荚膜是某些细菌在细胞壁外包围的一层粘液性物质,一般由糖和多肽组成,是细菌的一种特殊结构。
作用:
①抗吞噬作用:荚膜因其亲水性及其空间占位、屏障作用,可有效抵抗寄主吞噬细胞的吞噬作用。
②黏附作用:荚膜多糖可使细菌彼此间粘连,也可黏附于组织细胞或无生命物体表面,是引起感染的重要因素,具有荚膜的S-型肺炎链球菌毒力强,有助于肺炎链球菌侵染人体;废水生物处理中的细菌荚膜有生物吸附作用,将废水中的有机物、无机物及胶体吸附在细菌体表面上。
③抗有害物质的损伤作用:处于细菌细胞最外层,荚膜犹如盔甲可有效保护菌体免受或少受多种杀菌、抑菌物质的损伤,如溶菌酶、补体等。
④抗干燥作用:荚膜多糖为高度水合分子,含水量在95%以上,可帮助细菌抵抗干燥对生存的威胁。
⑤当缺乏营养时,荚膜可被利用作碳源和能源,有的荚膜还可作氮源。
所以一个细菌或病毒可以有很多的抗原。

