Solution preparation relies upon the precise measurement of volumes to obtain the exact concentrations required. Therefore, the choice of micropipette that is used can determine not only the accuracy - but also the reproducibility of your experiments.
At Ossila, we offer a selected range of micropipettes spanning from 1 μl all the way up to 10 ml. Each micropipette comes pre-calibrated and tested, to ensure accuracy and reproducibility. Additionally, we have prepared a handy guide on how to properly use your micropipette to get the best results possible.
- List of products and specifications
- How to operate a micropipette
- Micropipette Stand
Product List and Specification
| Product Code | C2001V1 | C2001V2 | C2001V3 | C2001V4 |
| Volume Range | 1-10 μl | 10-100 μl | 100-1000 μl | 1-10 ml |
| Accuracy | ±2.5 - 1.0% | ±1.8 - 0.8% | ±1.5 - 0.6% | ±2.5 - 0.6% |
| Repeatability | ≤1.5 - 0.4% | ≤0.5 - 0.15% | ≤0.3 - 0.15% | ≤0.3 - 0.15% |
| Compatible Tips | C2002V1 | C2002V2 | C2002V3 | C2002V4 |
| Sterilization | Autoclave at 121°C for 20 Minutes | |||
| Package Content | 1 x Micropipette, 1 x Calibration Key, 1 x Instruction Manual, 1 x Quality Assurance Form, 1 x Bag of Pipettes | |||
| Package Type | Cardboard Box | |||
<Return to top
How to Operate a Micropipette
Correct operation of a micropipette will allow for the most accurate and reproducible measuring and dispensing of solutions. Incorrect operation of a micropipette can cause significant decreases in both the accuracy and reproducibility of the volume of solution being withdrawn or dispensed. Below is a visual guide on how to correctly use a micropipette to get the highest accuracy and longest lifetime out of your equipment:
When withdrawing solution, do not fully insert the tip. You should aim to have it around 1 cm into the solution to avoid withdrawing close from the surface and taking in air, and avoiding any contact between the micropipette and the solution.
When withdrawing viscous solutions, the plunger should be released very slowly to ensure that no air bubbles form within the tip (as this will give you a lower volume than indicated).
Never hold your micropipette with the tip facing upwards while you have solution in the tip. This may cause the solution to run back into the piston mechanism, which will damage and contaminate it.
When dispensing small volumes, you may need to either dispense directly into the solution by immersing the tip or by dispensing onto the side of the vial. The tip should be replaced after each dispense to avoid cross contamination.
If a micropipette is set to a volume outside of its stated operational range, the micropipette should be recalibrated to ensure that you are still getting the correct volumes.
If the micropipette is dropped, this may result in the need to recalibrate it.
<Return to top
Micropipette Stand
For storage of your micropipettes, we recommend using a stand that sets them vertically, allowing you to hold liquid within the tip without it running back into the tip cone. This reduces the chance of contamination, and increases the lifetime and accuracy of your equipment. Ossila offers a carousel stand with 9 individual slots, allowing you to store multiple micropipettes with easy access to each.
<Return to top
To the best of our knowledge the technical information provided here is accurate. However, Ossila assume no liability for the accuracy of this information. The values provided here are typical at the time of manufacture and may vary over time and from batch to batch.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
对于我们生物/医学实验ers来说，每天必用移液器；它的准确与否直接关系到实验结果的准确性及可重复性。可能在大家看来，玩“枪”就像吃饭喝水一样soeasy！但扫地僧的故事告诉我们：愈是看似简单的事物，却往往蕴藏着大道理。你真的知道吸液时该浸入液面以下多深吗？你能尽可能准确地移取Tween、高浓度蛋白溶液等粘稠、发泡的液体吗？长期使用后，你的移液器还准吗？你会自检移液器吗？
答案是No的小伙伴们，你们有任何关于移液器的问题都可以来问我，大家一起讨论共同进步哈~同时感谢丁香园交流平台和版主的支持！谢谢！
本活动技术支持来自普兰德（上海）贸易有限公司。
版主科研无止尽留言:
好帖
将用待吸液润洗过的移液管插入待吸液面下1～2cm处用吸耳球按上述操作方法吸取溶液（注意移液管插入溶液不能太深，并要边吸边往下插入，始终保持此深度）。当管内液面上升至标线以上约1～2cm处时，迅速用右手食指堵住管口（此时若溶液下落至标准线以下，应重新吸取），将移液管提出待吸液面，并使管尖端接触待吸液容器内壁片刻后提起，用滤纸擦干移液管或吸量管下端粘附的少量溶液。（在移动移液管或吸量管时，应将移液管或吸量管保持垂直，不能倾斜） 洗净移液管，放置在移液管架上。向左转|向右转
1、移液管是准确移取一定量溶液的量器（量出式量器，符号为Ex）. 它是一根细长而中间膨大的玻璃管在管的上端有一环形标线，膨大部分标有它的容积和标定时的温度.常用的移液管有10 mL、25 mL、50 mL、100mL等规格。
2、吸量管的全称是"分度吸量管",又称为刻度移液管. 它是带有分度线的量出式玻璃量器。用于移取非固定量的溶液。吸量管可分为以下四类：
（1） 规定等待时间15s的吸量管
这类吸量管零位在上,完全流出式,它的任意一分度线的容量定义为:在20℃时，从零线排放到该分度线所流出20℃水的体积(m1).当液面降到该分度线以上几毫米时,应按紧管口停止排液15s，再将液面调到该分度线。在量取吸量管的全容量溶液时,排放过程中水流不应受到限制，液面降至流液口处静止后，要等待15s，再移走吸量管。
（2） 不完全流出式吸量管。
不完全流出式吸量管均为零点在上形式，最低分度线为标称容量.这类吸量管的任一分度线相应的容量定义为：20℃时，从零线排放到该分度线所流出的20C水的体积(mL) 。
（3）完全流出式吸量管。
这类吸量管有零点在上和零点在下两种形式.其任一分度线相应的容量定义为:在20℃时,从分度线排放到流液口时所流出20℃水的体积(mL),液体自由流下,直到确定弯月面已降到流液口静止后,再脱离容器(指零点在下式);或者从零线排放到该分度线或流液口所流出20℃水的体积(指零点在上式)。
（4） 吹出式吸量管。
这类吸量管流速较快，且不规定等待时间。有零点在上和零点在下两种形式，均为完全流出式。吹出式吸量管的任意一分度线的容量定义为：在20℃时，从该分度线排放到流液口(指零点在下)所流出的或从零线排放到该分度线(指零点在上)所流出的20℃水的体积(m1)。使用过程中液面降至流液口并静止时，应随即将最后一滴残留的溶液一次吹出。
目前市场上还有一种标“快”字的吸量管，其容量精度与吹出式吸量管相近似。吹出式及快流速吸量管的精度低、流速快，适于在仪器分析实验中加试剂用，最好不用其移取标准溶液。
吸量管的其它操作方法同移液管。
四字原则：吸 擦 调 放
吸：用吸耳球吸取液体，四指拿移液管，食指扣于移液管上口。
擦：吸取后用滤纸擦干管壁。
调：放松食指使液体流出，调至刻度线，按紧。
放：沿器壁放入所需容器。
垂直是为了保证最后只剩下半滴时，移液管不计算在内的体积。如果移液管上没有写“吹”字，那么最后半滴可以不要。
移液管
用来准确移取一定体积的溶液的量器。移液管是一种量出式仪器，只用来测量它所放出溶液的体积。它是一根中间有一膨大部分的细长玻璃管。其下端为尖嘴状，上端管颈处刻有一条标线，是所移取的准确体积的标志。
一．设定移液体积：1.从大体积调节到小体积时，为正常调节方法，逆时针旋转刻度即可；2.从小体积调节至大体积时，可先顺时针调至超过设定体积的刻度，再回调至设定体积，这样可以保证最佳的精确度。
二．装配移液器吸头：1.单道移液器，将移液端垂直插入吸头，左右微微转动，上紧即可2.用移液器反复撞击吸头来上紧的方法是不可取的，这样操作会导致移液器部件因强烈撞击而松散，严重的情况会导致调节刻度的旋钮卡住。3.多道移液器，将移液器的第一道对准第一个吸头，倾斜插入，前后稍许摇动上紧，吸头插入后略超过O型环即可。
从上理解，
移液枪增量是增加移液体积。
