Name | 2x GoldStar Best PCR Master Mix (with dye) | ||||||||||||||||||
Cat. # | W0655-1 $ 39.00 (1 mL, 100 rxn, 20 ul/rxn) $159.00 (5 mL, 500 rxn, 20 ul/rxn) How to pay with  | ||||||||||||||||||
Application |
This product is for research use only. | ||||||||||||||||||
Specifications |
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Description and features | 2x GoldStar Best PCR Master Mix (with dye) is a convenient premixed 2x concentrated solution for PCR which include GoldStar Best DNA Polymerase, PCR Buffer, Mg2+, dNTPs, PCR Stabilizer and PCR Intensifier. The polymerase has a 5´→3´ DNA polymerase and a 5´→3´ exonuclease activity.It also possesses 3´→5´ exonuclease (proofreading) activity. The polymerase has a higher amplification efficiency and lower mispairing rate than routine Taq DNA polymerase. The GoldStar Best is a high reliable hotstart polymerase, which activity is inhibited at ambient temperatures by the chemical modificaiton. This prevents the formation of misprimed products and primer dimers at ambient temperatures. GoldStar Best Polymerase is activated by a 10 minutes, 95°C incubation.Optimized buffer system promotes the amplification of target fragment with high fidelity, high specificity, high amplification efficiency and high sensitivity. GoldStar Best DNA Polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for TA cloning. The amplification range of Es Taq is ~ 6 kb. This PCR Master Mix contains dye, and can directly run electrophoresis after PCR reaction. | ||||||||||||||||||
Shipping / Storage | Ship at 4℃. Store at -20℃ for up to 1 year and avoid freeze-thaw cycles. Stored at 4℃ for up to 3 months. | ||||||||||||||||||
Quality control | This product is tested for no exogenous nuclease activity;no host DNA contamination tested (by PCR);able to amplify single copy gene from multiple genomes; and no significant enzyme activity decrease after storing at 2 ~ 8oC for 3 months. | ||||||||||||||||||
Manual (protocol) |  | ||||||||||||||||||
Components |
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PCR reaction system |  Note: The recommended primer concentration for PCR is between 0.1-1.0 µM of each primer. The use of higher concentrations of primers can have higher amplification effect. Low primer concentration will generally ensure cleaner product and lower background. | ||||||||||||||||||
PCR reaction conditions |  Note:
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PCR result examination | This PCR Master Mix contains dye for electrophoresis.After PCR, directly load 5 µL of PCR product to agarose gel to run electrophoresis.No need to add loading buffer. | ||||||||||||||||||
 | Exosome Isolation | Purity > 95% |
| Protein Extraction | 1 min total protein, 40 min membrane protein |
| 3D Cell Culture Gel | 30% < mkt=""> |
| PCR Kits | 50% < mkt=""> |
| Beta-Hexosaminidase Activity Colorimetric Assay | Fast and sensitive, High-throughput |
| Endotoxin-Free Plasmid Kits | maxi, midi and mini-prep |
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各位好，最近构建慢病毒沉默载体，问题是提完质粒酶切验证是阳性，但是测序检测不到插进去的shRNA，已经重复连接转化多次，依旧没有得到阳性质粒，请大神帮忙分析下原因，感谢！！
例用微物测定孢克罗效价用孢克罗标准品用HPLC或UV测定则用照品；非西丁用作熔点校准物质用熔点标准品测定含量用照品即使同种物质标准品照品规格、标定及用途都能同（北京标准物质网）
n.[医] （对伤处等的） 针探，探查; [医] 探针，取样器; 探测仪;探头;
vt.探索，调查; 用探针（或探测器等）探查，探测;
vt.盘问; （用试探性袭击等）侦察（敌情） ; 用尖物刺穿（物件）; 用力使向前推进;
The more they probed into his background, the more inflamed their suspicions would become
他们越调查他的背景，疑团就越多。
请问大神，siRNA一定是瞬转；如果瞬转，比如只能维持个2-3天，那么如果我想观察药物A作用于siRNA干预的细胞的效果，但是这个药物A需要作用10天，那么我间隔2-3天加一点SIRNA，这样可以吗，谢谢大神了
①些物,RNA病毒没DNA,能用cDNA克隆；
②cDNA克隆易筛选,cDNA库包含非结构基克隆,且每cDNA克隆含mRNA信息；
③cDNA能细菌表达.cDNA仅代表某发育阶段表达遗传信息,基文库才包含物完整遗传信息.
