Name | 101Bio WB Stripping Solution | ||||||
Cat. # | P5W3 How to pay with ![]() Also can buy from : ![]() ![]() | ||||||
Application | This product is for maximum removal of antibodies from a membrane and preserving the integrity of the antigen in 12-15 minutes . This product is for research use only. | ||||||
Product Size | 250 ml | ||||||
Description | 101Bio WB Stripping Solution (stripping buffer) is designed to rapidly strip antibodies bound to antigens on Western blotting membranes using chemiluminescent substrate.One of the major advantages of chemiluminescent detection is high sensitivity and the ability to strip reagents from a blot and re-probe the same blot.Re-probing Western blots are very useful for optimizing the blotting conditions such as testing different antibody dilution and membrane blocking conditions. The key to this process is to use conditions that cause the release of antibody from the antigen without causing a significant amount of antigen to be released from the membrane. During the stripping procedure, small amount of antigen is inevitably lost from the membrane. It is important to minimize this loss by stripping the antibody under gentle conditions. 101Bio WB stripping solution can achieve maximum removal of antibodies from a membrane and preserving the integrity of the antigen in 12-15 minutes without using harsh conditions such as boiling and treated with strong reducing agents.A blot can be stripped and re-probed multiple times to visualize multiple proteins or to optimize experimental conditions without the need for running multiple gels.
| ||||||
Shipping / Storage | Ship and store at room temperature. | ||||||
Shelf Life | 24 months | ||||||
Manual (protocol) | ![]() | ||||||
Components |
|

| Name | Cat. # | Size | ||
|---|---|---|---|---|
| 101Bio HRP Substrate Kit | P5W1 | 30 ml /30 ml | ||
| 101Bio Antibody Enhancer | P5W2 | 30 ml | ||
| 101Bio WB Stripping Solution | P5W3 | 250 ml | ||
| 101Bio Ponceau S Stain | P5W4 | 250 ml | ||
| 101Bio Peroxidase Suppressor | P5W5 | 30 ml | ||
| 101Bio High Efficiency Protein Precipitation kit | P5W6 | 30 ml /30 ml | ||
| 101Bio Red Blood Cell Lysis Buffer | P5W7 | 250 ml | ||
| 101Bio WB Blocking Solution | P5W8 | 250 ml | ||
| 101Bio Denaturing Protein Solubilization Reagent | P5W9 | 10 ml | ||
| 101Bio Non-Denatured Protein Solubilization Reagent | P5W10 | 10 ml | ||
| 101Bio Protein Solubilization Reagent for MS | P5W11 | 2 ml | ||
| 101Bio SDS-Remover | P5W12 | 10 ml | ||
| 101Bio Albumin Depletion Reagent for Plasma and Serum | P5W13 | 10 ml | ||
| 101Bio Protein A+G Agarose Beads | W0349 | 1 ml | ||
| 101Bio Protease Inhibitor Cocktail (100x) | W2200 | 1 ml | ||
| 101Bio Yeast Total Protein Extraction Kit for SDS-PAGE | Y203-50 | 50 rxn |

![]() | Exosome Isolation | Purity > 95% |
![]() | Protein Extraction | 1 min total protein, 40 min membrane protein |
![]() | 3D Cell Culture Gel | 30% < mkt=""> |
![]() | PCR Kits | 50% < mkt=""> |
![]() | Beta-Hexosaminidase Activity Colorimetric Assay | Fast and sensitive, High-throughput |
![]() | Endotoxin-Free Plasmid Kits | maxi, midi and mini-prep |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
目前常用方法有:化学修饰法、反应动力学法、X-射线衍射法等.1.化学修饰法:此方法是研究最早,应用最广泛的方法.原则上讲,酶分子侧链上的各种基团
1970科家致癌RNA病毒发现种特殊RNA病毒聚合酶,该酶能RNA模板,根据碱基互补原则.程与般病毒转录向相反,故称逆转录,催化程酶称逆转录酶,发现哺乳物胚胎细胞裂淋巴细胞含.
逆转录病毒称携带逆转录酶病毒,先侵入宿主细胞病毒RNA模板,靠酶形DNA环化,合宿主细胞染色体原病毒形式宿主细胞代代传.
HIV(艾滋病病毒)典型逆转录病毒
聚合酶就是多功能的dna的制作机器
RNA聚合酶的作用位点:3'-5'-磷酸二酯键
DNA解旋酶的作用位点:DNA中互补碱基之间的氢键
要收集临床病人的血清做mRNA,每天收集然后集中检测,问题来了:保存的EP管要用DEPC处理过的吗,就算保存时用的无RNA酶的管子,可是抽血的真空管也没处理过啊。我现在就用普通的管子冻在-70了,请教大家这样行不行啊?
目前我准备做甲基化PCR,由于经费问题,可能无法购买高价的试剂盒。看过甲基化PCR的原理后,在考虑经过修饰后,是否可以用RT-PCR的试剂盒(有现成的)来做甲基化PCR。请大牛指导!另求各位推荐性价比高的亚硫酸盐修饰试剂盒,谢谢!







