| AC 45594inverse agonist of steroidogenic factor 1 (SF-1) |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
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- Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
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| Cas No. | 13037-86-0 | SDF | Download SDF |
| Chemical Name | 4-(heptyloxy)phenol | ||
| Canonical SMILES | OC1=CC=C(C=C1)OCCCCCCC | ||
| Formula | C13H20O2 | M.Wt | 208.3 |
| Solubility | <20.83mg l="" in="" dmso;=""><20.83mg l="" in="" ethanol=""> | Storage | Store at -20°C |
| Physical Appearance | Off-white crystalline solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
AC 45594 is a full inverse agonist of steroidogenic factor 1 (SF-1) with an IC50 value of 50~100 nM [1].
SF-1 is a constitutively nuclear hormone receptor. It is essential to the development of gonadal and adrenal glands. It acts as a shaping factor in sexual differentiation and determination. Its effects are exerted primarily via controlling steroid hormone synthesis [1].
Human adrenocortical H295R/TR SF-1 cells were overexpressing SF-1 in a Dox-inducible fashion. After a 4-day treatment with AC 45594, the proliferation of H295R/TR SF-1 cells cultured in basal conditions was inhibited in a dose-dependent manner. AC 45594 showed maximal effect at 10 µM. SF-1 overexpression induced cell proliferation. In the presence of Dox, incubation with AC 45594 at 1 µM markedly reduced the effect of SF-1 overexpression. Moreover, treatment with AC 45594 increased the percentage of sub-G1 (apoptotic) cells [2]. In human adrenocortical H295 cells, cAMP is able to induce the expression of StAR. This induction is dependent on the presence of SF-1. Incubation with AC 45594 dose-dependently reduced amounts of the StAR mRNA to basal levels. At the protein level, 24 h after the cAMP treatment, StAR was increased by 30-fold. But treatment with AC 45594 completely and dose-dependently reversed the cAMP-dependent activation of StAR [1].
No result regarding the effect of AC 45594 in animal bodies had been found.
References: [1]. Del Tredici AL, Andersen CB, Currier EA, et al. Identification of the first synthetic steroidogenic factor 1 inverse agonists: pharmacological modulation of steroidogenic enzymes. Molecular pharmacology, 2008, 73(3): 900-908.[2]. Doghman M, Cazareth J, Douguet D, et al. Inhibition of adrenocortical carcinoma cell proliferation by steroidogenic factor-1 inverse agonists. The Journal of Clinical Endocrinology & Metabolism, 2009, 94(6): 2178-2183.
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请教大家一个问题，不同厂家cIEF的Marker能混合使用吗？另外，如果我对其它厂家pI值为6.0的marker进行cIEF测定，那测得的pI值会等于6.0吗？谢谢！
做western时，看着膜上的Marker条带是直的，但是最后显影出来的目的蛋白的条带全部是歪的，这是为什么？两块胶都是这个问题，欲哭无泪呀！！！求高人指点一下，是什么操作不当导致的
已经重复做了4次了，还是曝出9条带。请求各位大神解析解析==
想看下人肿瘤组织内巨噬细胞的浸润情况，我以为一个CD11b就够了。咨询了一下抗体公司，竟然让我订三个抗体，分别是CD11b，CD68和CD163，后两者不是M1和M2的分型吗？
另外查了下CD11b也确实在别的一些免疫细胞有所表达，请教下，有专门的特异性抗体吗？没课题没经费，只能省省省了
英 [?mɑ:k?(r)] 美 [?mɑ:rk?(r)]n.标识，标记； 记号笔，阅卷人； 防守队员； 特征复数： markers
双语例句
Draw your child's outline with a heavy black marker.用浓黑色的毡笔画出你孩子的轮廓。2. He put a marker in his book and followed her out.
他在书里夹了一个书签后便随她出去了。
3. He placed a marker where the ball had landed.他在球落地的地方放了个标记。
4. Step off twenty feet and then place a marker in the ground.先步测20英尺,再在地上做出标记.
5. Step out ten feet and then put a marker in the ground.步测十英尺,然后在地上做出标记.
英 [ˈmɑ:kə(r)] 美 [ˈmɑ:rkə(r)]
n.标识，标记； 记号笔，阅卷人； 防守队员； 特征
复数： markers
双语例句
1. Draw your child's outline with a heavy black marker.
用浓黑色的毡笔画出你孩子的轮廓。
2. He put a marker in his book and followed her out.
他在书里夹了一个书签后便随她出去了。
3. He placed a marker where the ball had landed.
他在球落地的地方放了个标记。
4. Step off twenty feet and then place a marker in the ground.
先步测20英尺,再在地上做出标记.
5. Step out ten feet and then put a marker in the ground.
步测十英尺,然后在地上做出标记.
连续三次都这样，Marker的泳道的位置有很多亮条带，但Marker本来的条带是不亮的。我做了内参和目的，一张胶上左右两部分分别都加样品和Marker，切开转膜分别孵育，一块儿显影，内参的Marker就不会亮，只有目的那一张Marker会亮，昨天做的借了别人的Marker，显影出来还是有那个条带。
我的目的条带还没有Marker那部分的条带亮，Marker的部分非常亮。
