• 偶联物AlexaFluor®488.Ex:495nm,Em:519nm
• 经测试应用ICC/IF, FlowCyt, IHC-Frmoredetails
• 种属反应性
  与反应: Mouse,Rat,Human预测可用于: Sheep,Goat,Cat,Dog,Zebrafish,Marmoset(common)
• 免疫原

  SyntheticpeptidewithinHumanNeuNaa1-100(Cysteineresidue).Theexactsequenceisproprietary.Databaselink: A6NFN3

• 阳性对照
  • ICC/IF:NGF-differentiatedPC12cellsandU-87MGcells.IHC-Fr:RatBrain(Normal).FlowCyt:U-87MGcells.
• 常规说明

  Thisproductisarecombinantrabbitmonoclonalantibody.

  ProducedusingAbcam’sRaBMAb^® technology.RabMAb^® technologyiscoveredbythefollowingU.S.Patents,No.5,675,063and/or7,429,487.

  TheAlexaFluor^® dyeincludedinthisproductisprovidedunderanintellectualpropertylicensefromLifeTechnologiesCorporation.AsthisproductcontainstheAlexaFluor^® dye,thepurchaseofthisproductconveystothebuyerthenon-transferablerighttousethepurchasedproductandcomponentsoftheproductonlyinresearchconductedbythebuyer(whetherthebuyerisanacademicorfor-profitentity).AsthisproductcontainstheAlexaFluor^® dyethesaleofthisproductisexpresslyconditionedonthebuyernotusingtheproductoritscomponents,oranymaterialsmadeusingtheproductoritscomponents,inanyactivitytogeneraterevenue,whichmayinclude,butisnotlimitedtouseoftheproductoritscomponents:(i)inmanufacturing;(ii)toprovideaservice,information,ordatainreturnforpayment(iii)fortherapeutic,diagnosticorprophylacticpurposes;or(iv)forresale,regardlessofwhethertheyaresoldforuseinresearch.ForinformationonpurchasingalicensetouseproductscontainingAlexaFluor^® dyesforpurposesotherthanresearch,contactLifeTechnologiesCorporation,5791VanAllenWay,Carlsbad,CA92008USAor outlicensing@lifetech.com.

   

  Alternativeversionsavailable:

  Anti-NeuNantibody [EPR12763]-NeuronalMarker(ab177487)Anti-NeuNantibody(AlexaFluor^® 647) [EPR12763]-NeuronalMarker(ab190565)

性能

• 形式Liquid
• 存放说明Shippedat4°C.Storeat+4°Cshortterm(1-2weeks).Upondeliveryaliquot.Storeat-20°Clongterm.Avoidfreeze/thawcycle.StoreIntheDark.
• 存储溶液pH:7.20Preservative:0.02%SodiumazideConstituents:PBS,30%Glycerol,1%BSA
• 浓度100µl浓度为0.5mg/ml 
• 纯度AffinityPurified
• 克隆单克隆
• 克隆编号EPR12763
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • CellTypeMarker
   
  • Neuronmarker
   
  • Somamarker

  • Tags&CellMarkers
   
  • CellTypeMarkers
   
  • NeuroscienceMarkers
   
  • Neuronal

  • EpigeneticsandNuclearSignaling
   
  • Transcription
   
  • DomainFamilies
  • ForkheadBox
   
  • OtherFOXes

  • EpigeneticsandNuclearSignaling
   
  • Transcription
   
  • TranscriptionFactors

应用

靶标

• 功能RNA-bindingproteinthatregulatesalternativesplicingevents.
• 序列相似性Contains1RRM(RNArecognitionmotif)domain.
• 细胞定位Nucleus.Cytoplasm.

• Anti-NeuNantibody[EPR12763]-NeuronalMarker(AlexaFluor®488)图像

  • 

    FlowCytometry-Anti-NeuNantibody[EPR12763]-NeuronalMarker(AlexaFluor®488)(ab190195)

    OverlayhistogramshowingU-87MGcellsstainedwithab190195(redline).Thecellswerefixedwith80%methanol(5min)andthenpermeABIlizedwith0.1%PBS-Tweenfor20min.Thecellswerethenincubatedin1xPBS/10%normalgoatserum/0.3Mglycinetoblocknon-specificprotein-proteininteractionsfollowedbytheantibody(ab190195,1/500dilution)for30minat22°C.Isotypecontrolantibody(blackline)wasrabbitIgG(monoclonal)AlexaFluor®488usedatthesameconcentrationandconditionsastheprimaryantibody.Unlabelledsample(blueline)wasalsousedasacontrol.

    Acquisitionof>5,000eventswerecollectedusinga20mWArgonionlaser(488nm)and525/30bandpassfilter.

    ThisantibodygaveapositivesignalinU-87MGfixedwith4%formaldehyde(10min)/permeabilizedwith0.1%PBS-Tweenfor20minusedunderthesameconditions.

  • 

    Immunocytochemistry/Immunofluorescence-Anti-NeuN[EPR12763]antibody-NeuronalMarker(AlexaFluor®488)(ab190195)

    ab190195stainingNeuNinNGF-differentiatedPC12cells(7days).Thecellswerefixedwith100%methanol(5min)andthenblockedin1%BSA/10%normalgoatserum/0.3Mglycinein0.1%PBS-Tweenfor1h.Thecellswerethenincubatedwithab190195at1/50dilution(showningreen)and ab7291 (Mousemonoclonal[DM1A]toalphaTubulin)at1µg/mlovernightat+4°C,followedbyafurtherincubationatroomtemperaturefor1hwithanAlexaFluor^® 594Goatanti-Mousesecondary(ab150120)at2μg/ml(showninred).NuclearDNAwaslabelledinbluewithDAPI.

  • 

    Immunohistochemistry(Frozensections)-Anti-NeuNantibody[EPR12763]-NeuronalMarker(AlexaFluor®488)(ab190195)

    IHCimageofab190195staininginacetonefixed frozen tissuesectionof normalratbrain.

    Thesectionwaspre-treatedusingheatmediatedantigenretrievalwithsodiumcitratebuffer(pH6)inaDakoPascalpressurecookerusingthestandardfactory-setregime.Non-specificprotein-proteininteractionswerethenblockedusinginTBScontaining0.025%(v/v)TritonX-100,0.3M(w/v)glycineand3%(w/v)BSAfor1hatroomtemperature.Thesectionwasthenincubatedwithab190195(1/50)inTBScontaining0.025%(v/v)TritonX-100and3%(w/v)BSAovernightat+4°C.Thesectionwasthencounterstained andmountedwithSlowFade^® GoldAntifadeMountantwithDAPI.

    The DAPIonlycontrol (noantibody)insetshowsnoautofluorescence,demonstratingthatanyAlexaFluor^®488signalisdervieddirectlyfromboundab190195.Theseparateimagesofab190195andDAPIalone,combinedwiththemergedversionofbothsignals,shows predominantco-localisationofthe AlexaFluor^®488signalin thenucleiofthehippocampalgranularlayer.

    ForotherIHCstainingsystems(automatedandnon-automated),customersshouldoptimizevariableparameterssuchasantigenretrievalconditions,antibodyconcentrationsandincubationtimes.

  • 

    Immunocytochemistry/Immunofluorescence-Anti-NeuNantibody[EPR12763]-NeuronalMarker(AlexaFluor®488)(ab190195)

    ab190195stainingNeuNinU87-MGcells.Thecellswerefixedwith100%methanol(5min)andthenblockedin1%BSA/10%normalgoatserum/0.3Mglycinein0.1%PBS-Tweenfor1h.Thecellswerethenincubatedwithab190195at 1/50dilution (showningreen)and ab7291(Mousemonoclonal[DM1A]toalphaTubulin)at1µg/mlovernightat+4°C,followedbyafurtherincubationatroomtemperaturefor1hwithanAlexaFluor^® 594Goatanti-Mousesecondary(ab150120)at2μg/ml(showninred).NuclearDNAwaslabelledinbluewithDAPI.