- Tip Material: Stainless Steel
- For use with NanoFil syringes
- Package of 2
- Assortment packages are available
Click here to view the current NanoFil Data Sheet.
Options
| Order code | Description | Gauge | Bevel/Blunt | Pkg Size |
| NF33BL-2 | Nanofil Needle | 33 G | Blunt | 2 |
| NF34BL-2 | Nanofil Needle | 34 G | Blunt | 2 |
| NF35BL-2 | Nanofil Needle | 35 G | Blunt | 2 |
| NF36BL-2 | Nanofil Needle | 36 G | Blunt | 2 |
| NF33BV-2 | Nanofil Needle | 33 G | Beveled | 2 |
| NF34BV-2 | Nanofil Needle | 34 G | Beveled | 2 |
| NF35BV-2 | Nanofil Needle | 35 G | Beveled | 2 |
| NF36BV-2 | Nanofil Needle | 36 G | Beveled | 2 |
| NF33-36BL | Nanofil Needle Assortment | 33-36 G | Blunt | 4 (1 of each size) |
| NF33-36BV | Nanofil Needle Assortment | 33-36 G | Beveled | 4 (1 of each size) |
Selecting the Correct Tip for your Application
The replaceable needles used with the NanoFil are available with either blunt or beveled tips. The blunt tip is used for injection into soft tissue and when a uniform solution distribution is needed. The beveled style is used for applications that involve the penetration of a tough tissue.
One of the main factors that limit the resolution and accuracy of conventional micro syringes to the upper tens of nanoliters range is diffusion in the large tip ID. When the tip ID is equal or larger than 100μm, the error caused by tip diffusion is in the nanoliter range level [(100μm)3 = 1 nL]. With a 36 gauge needle installed on the NanoFil, the error caused by diffusion will be reduced to the sub-nanoliter level, making accurate injection of a nanoliter possible.
All of WPI’s beveled tips have a unique 25º tri-surface bevel that is optimized for microinjection. A 10º single-surface beveled tip penetrates better than one with a 25º angle, however the distance between the upper opening to the tip (the dimension “F” in the drawing above) is longer. As a result, it requires a deeper penetration of the tip to achieve the same level of liquid delivery. Deeper penetration means more tissue damage. WPI’s unique 25º beveled tip solvesthis problem with two extra beveled surfaces. The tip of a single surface beveled tip is actually a blade instead of a point. It dulls very quickly. In contrast, the tri-surfaced tip has a real point. It not only penetrates much better but is also much more durable. Our tests show that our 33 gauge, 25º beveled tip penetrates easier and lasts longer than other manufacturers’ 33 gauge, 10º single beveled tips. With a 35 gauge tri-surface beveled tip, the resistance to the penetration becomes even less. Each of our tips undergo a penetration test before leaving the factory to guarantee the best results for our customers.
Available Tips
33 gauge: This tip is similar to Hamilton’s 7762 and 7803 series removable needles in both tip length and outer diameter. However, our beveled tip version is shorter, more durable, and penetrates better due to the special tri-surface grinding technique. In the past, 33 gauge tips were the smallest size sold by other manufacturers and were frequently cited in literature. However, our new 35 gauge tip is much better for injections involving small animals, especially mice. Compared with Hamilton’s 33 gauge, 10 degree beveled tip, our 35 gauge 25 degree beveled tip can reduce the depth of penetration by almost 80%. The distance between the tip and the upper rim of the opening (dimension F on the drawing) is 348 microns for the 33 gauge tip. The distance for our 35 gauge tip is only 230 microns. In addition, the smaller tip size significantly reduces the required penetration force. In nearly all applications, a 33 gauge tip can be replaced with our 35 gauge tip and produce better results.
34 gauge: This is a transitional size between the 33 gauge and 35 gauge. If the 35 gauge is too weak and the 33 gauge is too large, this makes a good alternative.
35 gauge: This was the most popular and preferred tip of most scientists during our field trial. The combination of its strength, length, durability, and clogging resistance creates a balance with very little compromising of the individual properties. It is much smaller than the 33 gauge tip offered by other manufacturers. It is only slightly larger than the 36 gauge tip but is much stronger and less likely to be clogged. Samples can be directly loaded with this tip. Its 5 mm length is sufficient enough for almost all injection applications in mice.
36 gauge: This is the smallest tip that is commercially available. The tip is so small that it can be inserted into the opening of the 33 gauge needle tip. Because this is pushing the limits of what current technology can produce, there are some limitations to consider before using. Its thin diameter makes it necessary to limit its length to 2.5 to 3 mm in order to maintain a usable strength. Since the tip ID is in the 25 to 50 micron range, it is very easily clogged. Therefore, only well filtered solutions can be used. Depending on the viscosity of the sample, the user might also need to pre-load the syringe with a regular tip before switching to this tip for injection. We recommend using the 35 gauge tip instead of the 36 gauge unless it is absolutely necessary.
NanoFil Microsyringe for Nanoliter Injection
| Tip Order Number | Guage | Tip O.D. “A” | Tip I.D. “B” | Tip Length “C” | Total Length “D” | Shank O.D. “E” | Bevel Length “F” | Total Dead Volume | Tip Material |
| NF33BV-2 | 33 | 210 μm | 115 μm | 10 mm | 40 mm | 460 μm | ≈348 µm | 0.416 µL | Stainless Steel |
| NF34BV-2 | 34 | 185 μm | 85 μm | 5 mm | 35 mm | 460 μm | ≈290 µm | 0.199 µL | Stainless Steel |
| NF35BV-2 | 35 | 135 μm | 55 μm | 5 mm | 35 mm | 460 μm | ≈204 µm | 0.435 µL | Stainless Steel |
| NF36BV-2 | 36 | 120 μm | 35 μm | 3 mm | 33 mm | 460 μm | ≈156 µm | 0.340 µL | Stainless Steel |
| NF33BL-2 | 33 | 210 µm | 115 µm | 10 mm | 40 mm | 460 µm | ≈0 | 0.416 µL | Stainless Steel |
| NF34BL-2 | 34 | 185 µm | 85 µm | 5 mm | 35 mm | 460 µm | ≈0 | 0.199 µL | Stainless Steel |
| NF35BL-2 | 35 | 135 µm | 55 µm | 5 mm | 35 mm | 460 µm | ≈0 | 0.435 µL | Stainless Steel |
| NF36BL-2 | 36 | 120 µm | 35 µm | 3 mm | 33 mm | 460 µm | ≈0 | 0.340 µL | Stainless Steel |
Goel M, Sienkiewicz AE, Picciani R, Wang J, Lee RK, Bhattacharya SK, "Cochlin, intraocular pressure regulation and mechanosensing." PLoS One. 2012;7(4):e34309. Epub 2012 Apr 4.
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2、电泳问题：如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
我分别用3ul、5ul、8ul都试过了，没有任何条带，用别人别的方法提好的DNA做，却能出现结果，不知道是自己提取的DNA浓度不够，还是根本没有提取出来。
不知道谁用过，或者有更好的全血提取NDA的试剂盒推荐呢？
本人刚刚开始做PCR，谢谢各位高手！
各位战友好，因实验要求需要提取人的唾液DNA，但因标本需要在临床采集，无法做到当天就提取DNA，因此天根等漱口水提DNA的试剂盒都不满足实验需求。
之前一直在用艾德莱的唾液DNA保存运输提取试剂盒，但是这段时间下来感觉提取效率太低，而且特别不稳定，经常50%以上的标本都提不出来DNA，或者浓度特别低，完全崩溃的感觉！所以想更换试剂盒或者直接提供唾液DNA的保存液。另国外的这种试剂盒太贵了，因经费问题无法承受，所以希望战友们能够推荐一家效率高些的试剂盒，小女子不胜感激！！
便宜点的就是国产的tiangen，效果会差一点点 如果是大样本的全血 用这个也凑合
