- Tip Material: Stainless Steel
- For use with NanoFil syringes
- Package of 2
- Assortment packages are available
Click here to view the current NanoFil Data Sheet.
Options
| Order code | Description | Gauge | Bevel/Blunt | Pkg Size |
| NF33BL-2 | Nanofil Needle | 33 G | Blunt | 2 |
| NF34BL-2 | Nanofil Needle | 34 G | Blunt | 2 |
| NF35BL-2 | Nanofil Needle | 35 G | Blunt | 2 |
| NF36BL-2 | Nanofil Needle | 36 G | Blunt | 2 |
| NF33BV-2 | Nanofil Needle | 33 G | Beveled | 2 |
| NF34BV-2 | Nanofil Needle | 34 G | Beveled | 2 |
| NF35BV-2 | Nanofil Needle | 35 G | Beveled | 2 |
| NF36BV-2 | Nanofil Needle | 36 G | Beveled | 2 |
| NF33-36BL | Nanofil Needle Assortment | 33-36 G | Blunt | 4 (1 of each size) |
| NF33-36BV | Nanofil Needle Assortment | 33-36 G | Beveled | 4 (1 of each size) |
Selecting the Correct Tip for your Application
The replaceable needles used with the NanoFil are available with either blunt or beveled tips. The blunt tip is used for injection into soft tissue and when a uniform solution distribution is needed. The beveled style is used for applications that involve the penetration of a tough tissue.
One of the main factors that limit the resolution and accuracy of conventional micro syringes to the upper tens of nanoliters range is diffusion in the large tip ID. When the tip ID is equal or larger than 100μm, the error caused by tip diffusion is in the nanoliter range level [(100μm)3 = 1 nL]. With a 36 gauge needle installed on the NanoFil, the error caused by diffusion will be reduced to the sub-nanoliter level, making accurate injection of a nanoliter possible.
All of WPI’s beveled tips have a unique 25º tri-surface bevel that is optimized for microinjection. A 10º single-surface beveled tip penetrates better than one with a 25º angle, however the distance between the upper opening to the tip (the dimension “F” in the drawing above) is longer. As a result, it requires a deeper penetration of the tip to achieve the same level of liquid delivery. Deeper penetration means more tissue damage. WPI’s unique 25º beveled tip solvesthis problem with two extra beveled surfaces. The tip of a single surface beveled tip is actually a blade instead of a point. It dulls very quickly. In contrast, the tri-surfaced tip has a real point. It not only penetrates much better but is also much more durable. Our tests show that our 33 gauge, 25º beveled tip penetrates easier and lasts longer than other manufacturers’ 33 gauge, 10º single beveled tips. With a 35 gauge tri-surface beveled tip, the resistance to the penetration becomes even less. Each of our tips undergo a penetration test before leaving the factory to guarantee the best results for our customers.
Available Tips
33 gauge: This tip is similar to Hamilton’s 7762 and 7803 series removable needles in both tip length and outer diameter. However, our beveled tip version is shorter, more durable, and penetrates better due to the special tri-surface grinding technique. In the past, 33 gauge tips were the smallest size sold by other manufacturers and were frequently cited in literature. However, our new 35 gauge tip is much better for injections involving small animals, especially mice. Compared with Hamilton’s 33 gauge, 10 degree beveled tip, our 35 gauge 25 degree beveled tip can reduce the depth of penetration by almost 80%. The distance between the tip and the upper rim of the opening (dimension F on the drawing) is 348 microns for the 33 gauge tip. The distance for our 35 gauge tip is only 230 microns. In addition, the smaller tip size significantly reduces the required penetration force. In nearly all applications, a 33 gauge tip can be replaced with our 35 gauge tip and produce better results.
34 gauge: This is a transitional size between the 33 gauge and 35 gauge. If the 35 gauge is too weak and the 33 gauge is too large, this makes a good alternative.
35 gauge: This was the most popular and preferred tip of most scientists during our field trial. The combination of its strength, length, durability, and clogging resistance creates a balance with very little compromising of the individual properties. It is much smaller than the 33 gauge tip offered by other manufacturers. It is only slightly larger than the 36 gauge tip but is much stronger and less likely to be clogged. Samples can be directly loaded with this tip. Its 5 mm length is sufficient enough for almost all injection applications in mice.
36 gauge: This is the smallest tip that is commercially available. The tip is so small that it can be inserted into the opening of the 33 gauge needle tip. Because this is pushing the limits of what current technology can produce, there are some limitations to consider before using. Its thin diameter makes it necessary to limit its length to 2.5 to 3 mm in order to maintain a usable strength. Since the tip ID is in the 25 to 50 micron range, it is very easily clogged. Therefore, only well filtered solutions can be used. Depending on the viscosity of the sample, the user might also need to pre-load the syringe with a regular tip before switching to this tip for injection. We recommend using the 35 gauge tip instead of the 36 gauge unless it is absolutely necessary.
NanoFil Microsyringe for Nanoliter Injection
| Tip Order Number | Guage | Tip O.D. “A” | Tip I.D. “B” | Tip Length “C” | Total Length “D” | Shank O.D. “E” | Bevel Length “F” | Total Dead Volume | Tip Material |
| NF33BV-2 | 33 | 210 μm | 115 μm | 10 mm | 40 mm | 460 μm | ≈348 µm | 0.416 µL | Stainless Steel |
| NF34BV-2 | 34 | 185 μm | 85 μm | 5 mm | 35 mm | 460 μm | ≈290 µm | 0.199 µL | Stainless Steel |
| NF35BV-2 | 35 | 135 μm | 55 μm | 5 mm | 35 mm | 460 μm | ≈204 µm | 0.435 µL | Stainless Steel |
| NF36BV-2 | 36 | 120 μm | 35 μm | 3 mm | 33 mm | 460 μm | ≈156 µm | 0.340 µL | Stainless Steel |
| NF33BL-2 | 33 | 210 µm | 115 µm | 10 mm | 40 mm | 460 µm | ≈0 | 0.416 µL | Stainless Steel |
| NF34BL-2 | 34 | 185 µm | 85 µm | 5 mm | 35 mm | 460 µm | ≈0 | 0.199 µL | Stainless Steel |
| NF35BL-2 | 35 | 135 µm | 55 µm | 5 mm | 35 mm | 460 µm | ≈0 | 0.435 µL | Stainless Steel |
| NF36BL-2 | 36 | 120 µm | 35 µm | 3 mm | 33 mm | 460 µm | ≈0 | 0.340 µL | Stainless Steel |
Goel M, Sienkiewicz AE, Picciani R, Wang J, Lee RK, Bhattacharya SK, "Cochlin, intraocular pressure regulation and mechanosensing." PLoS One. 2012;7(4):e34309. Epub 2012 Apr 4.
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普通的手提或者试剂盒提取的土壤基因组DNA由于常常残留有PCR的强烈抑制物如腐殖酸、棕黄酸等杂质造成实验失败，此外，由于采用了剧烈的玻璃珠击打来破裂菌体，常常造成DNA剪切和降解。本公司经过长期研发开发出了具有自主知识产权的土壤基因组DNA，通过专利配方的腐殖酸和棕黄酸去除试剂配合特殊处理的纯化柱，可以最大程度的去除这些杂质，同时加上多次柱漂洗，确保得到的DNA具有极高纯度，此外独特的抽提和裂解体系可以迅速裂解细胞（壁）和灭活细胞内核酸酶，不需要借助玻璃珠破壁，有效保证了基因组DNA的完整性。
产品特点：
1.本公司独有的专利配方和纯化柱能有效去除腐殖酸等杂质。
2.不需要借助玻璃珠破壁，有效保证了基因组DNA的完整性，长度可达30kb -50kb，可直接用于PCR，Southern-blot和各种酶切反应。
3.兼容性强，适用于各种不同的土壤包括淤泥等提取困难的土壤。
4.多步骤去除各种杂质和抑制物，保证了极高纯度，OD260/OD280典型的比值达1.7～1.9。
5.不需要使用有毒的苯酚等试剂，也不需要乙醇沉淀等步骤。
6.快速，简捷，单个样品操作一般可在60分钟内完成。
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