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Addgene/pAAV-hSyn-EGFP/1unit/50465-AAVrg
免费咨询热线
4000-520-616
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 50465 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
| AAV1 | 50465-AAV1 | Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV1 trial size | 50465-AAV1.T | Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV2 | 50465-AAV2 | Virus (100 µL at titer ≥ 3×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV2 trial size | 50465-AAV2.T | Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV5 | 50465-AAV5 | Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV5 trial size | 50465-AAV5.T | Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV8 | 50465-AAV8 | Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV8 trial size | 50465-AAV8.T | Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV9 | 50465-AAV9 | Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV9 trial size | 50465-AAV9.T | Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV Retrograde | 50465-AAVrg | Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV Retrograde trial size | 50465-AAVrg.T | Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV(Search Vector Database)
- Backbone sizew/o insert(bp)4818
- Total vector size (bp)5265
- Vector typeAAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature30°C
- Growth Strain(s)NEB Stable
- Copy numberLow Copy
Gene/Insert
- Gene/Insert nameEGFP
- SpeciesAequorea victoria
- Insert Size (bp)726
- Promoterhuman Synapsin 1
- Tag/ Fusion Protein
- N/A
Cloning Information
- Cloning methodRestriction Enzyme
- 5′ cloning siteBamH I(not destroyed)
- 3′ cloning siteEcoR I(not destroyed)
- 5′ sequencing primerTCGTGTCGTGCCTGAGAGCG
- 3′ sequencing primerGCATTAAAGCAGCGTATCCACATAGC (Common Sequencing Primers)
Resource Information
- Terms and Licenses
- UBMTA
- Ancillary Agreement for Plasmids Containing FP Materials
- Industry Terms
- Not Available to Industry
- Articles Citing this Plasmid
- 18 References
Depositor Comments
For more information, see: http://pdspit3.mml.unc.edu/projects/dreadd/wiki/WikiStart
Information for AAV1 (Catalog # 50465-AAV1)(Back to top)
Purpose
Ready-to-use AAV1 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA.
hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
- BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
- SerotypeAAV1
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV1 trial size (Catalog # 50465-AAV1.T)(Back to top)
Purpose
Ready-to-use AAV1 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA.
hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume20 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
- BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
- SerotypeAAV1
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV2 (Catalog # 50465-AAV2)(Back to top)
Purpose
Ready-to-use AAV2 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA.
hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 3×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
- BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
- SerotypeAAV2
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Addgene Comments
Data submitted about 50465-AAV2 by requesting scientist(s):
- Data 1: Rat, Brain parenchyma
Information for AAV2 trial size (Catalog # 50465-AAV2.T)(Back to top)
Purpose
Ready-to-use AAV2 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA.
hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume20 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
- BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
- SerotypeAAV9
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV5 (Catalog # 50465-AAV5)(Back to top)
Purpose
Ready-to-use AAV5 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA.
hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV5
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV5 trial size (Catalog # 50465-AAV5.T)(Back to top)
Purpose
Ready-to-use AAV5 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA.
hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume20 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
- BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
- SerotypeAAV5
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV8 (Catalog # 50465-AAV8)(Back to top)
Purpose
Ready-to-use AAV8 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA.
hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV8
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV8 trial size (Catalog # 50465-AAV8.T)(Back to top)
Purpose
Ready-to-use AAV8 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA.
hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume20 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV8
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV9 (Catalog # 50465-AAV9)(Back to top)
Purpose
Ready-to-use AAV9 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA.
hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV9
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV9 trial size (Catalog # 50465-AAV9.T)(Back to top)
Purpose
Ready-to-use AAV9 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA.
hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume20 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
- BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
- SerotypeAAV9
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV Retrograde (Catalog # 50465-AAVrg)(Back to top)
Purpose
Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA.
Synapsin-driven EGFP-expression control. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)
- BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
- SerotypeAAV retrograde (AAVrg)
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Addgene Comments
Retrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use.Information for AAV Retrograde trial size (Catalog # 50465-AAVrg.T)(Back to top)
Purpose
Ready-to-use AAV Retrograde trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA.
hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.Delivery
- Volume20 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)
- BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
- SerotypeAAV retrograde (AAVrg)
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneEGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
Quality Control:
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Addgene Comments
Retrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use.蚂蚁淘电商平台
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蚂蚁淘所有产品都是自运营的，我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权； 同时客户可以通过订单详情查看到货物从厂方至客户的所有流程， 确保货物的来源； 正规报关，提供13%增值税发票。
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2018-10-23
苏州泓迅生物科技股份有限公司在发布的杂交瘤细胞抗体基因测序供应信息，浏览与杂交瘤细胞抗体基因测序相关的产品或在搜索更多与杂交瘤细胞抗体基因测序相关的内容。 查看更多
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2018-12-22
在今天发表在科学报告上的一篇论文中，基尤皇家植物园的研究人员首次详细介绍了现在可通过便携式实时DNA测序获得植物科学的机会。Kew科学家和论文的合着者Joe Parker说; “这项研究证明，我们现在可以快速读取生物体的DNA序列，用最少的设备识别它。随时随地快速阅读DNA应成为许多研究领域的常规步骤。尽管进行了数百年的分类学研究，通过观察植物所属的物种仍然并不总是很容易找到。很少有人能够正确识别自己园中的所有物种。“在过去的四十年中，DNA测序已经彻底改变了科 查看更多
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2018-12-26
Methods for DNA sequencingBst DNA polymerase-catalyzed radiolabeled two-step sequencing reactions (26) are modified from those presented earlier (25) by alteri 查看更多
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2018-12-26
最糟糕的心态 - 实验失败了，抱怨试剂不好。实验人员本来是应该拥有修正主义、机会主义、怀疑论等诸多“不完美的”意识，所以一定要用“完美的”自我批评意识来平衡。我为什么没有一双慧眼选择可靠的供应商？我为什么不做预实验检测所购试剂？最糟糕的知识 - RNase A 的作用。RNase A 是内切酶，内 查看更多
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2021-08-18
罗氏通过接管基因序列专业公司 Kapa Biosystems 而巩固了与其长期的合作伙伴关系。这家瑞士制药巨头自 2013 年以来一直与 Kapa 有合作，并在去年底扩大了双方的合作范围，Kapa 同意为罗氏旗下 NimbleGen 单元生产定制的 RNA-seq 样品制备套组。罗氏现在已同意收购私人持有的 Kapa 来「加强其新一代测序（NGS）产品供应，」而两年前，罗氏打算寻求收购 Illumina，但最终未能成功。自那 查看更多
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2018-12-26
Template PreparationThe quality of sequencing results is directlyrelated to the quality of the template. ABI recommends a minialkaline-lysis/PEG precipitation 查看更多
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2017-06-12
基因测序让我们的生活更加美好！ 查看更多
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2021-08-31
自2013年5月14日，安吉丽娜朱莉(Angelina Jolie)在《纽约时报》上发表的公开信《我的医疗选择》(MyMedical Choice)中宣布自己因有基因缺陷，进行了预防性的双乳腺切除及乳房再造手术之后， 查看更多
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2018-07-15
【cDNA文库构建/EST测序分析技术服务】生物实验,分子生物学实验技术,BIOLEAF.实验技术服务 tosciencebio@126.com 选实验技术服务，还在上海通善。 您的选择，就是对我们公司的肯定。 相信通善，相信自己，我们有太多优势，让您在众多信息流中独具慧眼的找到我们。【cDNA文库构建/EST测序分析技术服务】生物实验,分子生物学实验技术,BIOLEAF.实验技术服务 tosciencebio@126.com 一， 查看更多
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2017-06-07
表观遗传学研究的起航点，开辟预防优于治疗的新时代！ 查看更多
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2021-08-15
导读： 基因测序 市场的下一个爆发点在哪里呢?我国已宣布启动精准医疗计划，基因检测被视为精确打击肿瘤的重要手段，那么肿瘤基因的液态活检技术，必将成为下一个巨大市场机会 查看更多
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2018-03-20
上海迪奥生物科技有限公司是人cDNA文库(15000条)技术服务商之一，从事人cDNA文库(15000条)已经多年。同时，生物在线为您提供众多企业人cDNA文库(15000条)技术服务，您可以搜索更多相关的人cDNA文库(15000条)实验技术服务，以便挑选到性价比高，合适的人cDNA文库(15000条)产品。而生物在线将会为您在人cDNA文库(15000条)方面提供全方位的解决方案。 查看更多
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商品咨询
TruSeq DNA PCRFree Sample:TruSeq DNA PCR免费样品123
加菲14日1652017-10-02
PCRfree顾名思义，就是不使用PCR扩增而直接构建文库，需要特定的试剂盒，这样的文库免除了因扩增导致的错误，没有偏好性，不过成本相对高一些
【求助】新手小白弱弱的问个问题:溶解DNA的水大家都怎么制备的? ...123
夏风微2021-07-22
【求助】请教DOPPCR和ALUPCR在扩增BAC/PAC DNA制备探针...123
hansontcm2021-08-03
请教DOP-PCR和ALU-PCR在扩增BAC/PACDNA制备探针文库时的优劣，或者说都可以。
核酸(DNA/RNA)提取试剂盒(磁珠法)和核酸(DNA/RNA)提取试剂盒(磁珠法...123
没事看看00322021-08-02
目前abi的是比较好的
全血DNA提取试剂盒哪个公司最好? 123
njlinger2004-10-27
我用的是华美生物工程公司全血基因组DNA纯化系统试剂盒，说明书上说利用A260光吸测定浓度对该方法制备的基因组DNA是不适用的，产量可达1ug/20ul全血，5-10ul上清液可以直接用于PCR扩增。
我分别用3ul、5ul、8ul都试过了，没有任何条带，用别人别的方法提好的DNA做，却能出现结果，不知道是自己提取的DNA浓度不够，还是根本没有提取出来。
不知道谁用过，或者有更好的全血提取NDA的试剂盒推荐呢？
本人刚刚开始做PCR，谢谢各位高手！
我分别用3ul、5ul、8ul都试过了，没有任何条带，用别人别的方法提好的DNA做，却能出现结果，不知道是自己提取的DNA浓度不够，还是根本没有提取出来。
不知道谁用过，或者有更好的全血提取NDA的试剂盒推荐呢？
本人刚刚开始做PCR，谢谢各位高手！
试剂盒DNA提取详细操作步骤 123
ballance1﹐k鍃2017-10-03
室温。低温保存可能会有沉淀析出。如果发生析出现象，请于50℃溶解后，再于室温保存。向左转|向右转
关于土壤DNA提取的试剂盒,哪个好点,价格也合适 微生物 ...123
猴耸挂92021-08-05
产品介绍：
普通的手提或者试剂盒提取的土壤基因组DNA由于常常残留有PCR的强烈抑制物如腐殖酸、棕黄酸等杂质造成实验失败，此外，由于采用了剧烈的玻璃珠击打来破裂菌体，常常造成DNA剪切和降解。本公司经过长期研发开发出了具有自主知识产权的土壤基因组DNA，通过专利配方的腐殖酸和棕黄酸去除试剂配合特殊处理的纯化柱，可以最大程度的去除这些杂质，同时加上多次柱漂洗，确保得到的DNA具有极高纯度，此外独特的抽提和裂解体系可以迅速裂解细胞（壁）和灭活细胞内核酸酶，不需要借助玻璃珠破壁，有效保证了基因组DNA的完整性。
产品特点：
1.本公司独有的专利配方和纯化柱能有效去除腐殖酸等杂质。
2.不需要借助玻璃珠破壁，有效保证了基因组DNA的完整性，长度可达30kb -50kb，可直接用于PCR，Southern-blot和各种酶切反应。
3.兼容性强，适用于各种不同的土壤包括淤泥等提取困难的土壤。
4.多步骤去除各种杂质和抑制物，保证了极高纯度，OD260/OD280典型的比值达1.7～1.9。
5.不需要使用有毒的苯酚等试剂，也不需要乙醇沉淀等步骤。
6.快速，简捷，单个样品操作一般可在60分钟内完成。
你找下，杭州昊鑫生物
普通的手提或者试剂盒提取的土壤基因组DNA由于常常残留有PCR的强烈抑制物如腐殖酸、棕黄酸等杂质造成实验失败，此外，由于采用了剧烈的玻璃珠击打来破裂菌体，常常造成DNA剪切和降解。本公司经过长期研发开发出了具有自主知识产权的土壤基因组DNA，通过专利配方的腐殖酸和棕黄酸去除试剂配合特殊处理的纯化柱，可以最大程度的去除这些杂质，同时加上多次柱漂洗，确保得到的DNA具有极高纯度，此外独特的抽提和裂解体系可以迅速裂解细胞（壁）和灭活细胞内核酸酶，不需要借助玻璃珠破壁，有效保证了基因组DNA的完整性。
产品特点：
1.本公司独有的专利配方和纯化柱能有效去除腐殖酸等杂质。
2.不需要借助玻璃珠破壁，有效保证了基因组DNA的完整性，长度可达30kb -50kb，可直接用于PCR，Southern-blot和各种酶切反应。
3.兼容性强，适用于各种不同的土壤包括淤泥等提取困难的土壤。
4.多步骤去除各种杂质和抑制物，保证了极高纯度，OD260/OD280典型的比值达1.7～1.9。
5.不需要使用有毒的苯酚等试剂，也不需要乙醇沉淀等步骤。
6.快速，简捷，单个样品操作一般可在60分钟内完成。
你找下，杭州昊鑫生物
【求助】Takara 的cDNA文库构建试剂盒??123
壮哉我ac122652021-07-24
是单链cDNA，想合成双链cDNA需要另外操作：一、cDNA第二链的合成:1. 第一链反应完成后,取2ul一链产物－20℃冰箱中保存，待电泳检测。其余的产物合并，混匀，然后顺序加入下列试剂(promega):20ul 10×DNA Polymerase I buffer6ul10mM dNTP(自己配制)xuldd H2O1ulRNase H(2U/ul)10ul DNA Polymerase I(10U/ul)总体系为200ul;2. 混匀后,16℃反应2.5小时;3. 70℃灭活10分钟;4. 反应完成后,得到200ul cDNA第二链反应体系,将此体系置于冰上;5．取2ul二链产物，同保存的一链产物一起电泳鉴定。同时上1kb ladder，确定双链的大小范围。注：一链，二链的电泳图是smear，且二链稍比一链大一些。二、双链cDNA末端补平:1. 在第二链反应体系中,顺序加入下列试剂(promega):6ul 10mM dNTP2ul T4 DNA Polymerase(8.7U/ul) 2ul BSA(10mg/ml)2. 稍微离心混匀反应物, 37℃反应至少30分钟,然后75℃灭活10分钟;3. 加入等体积酚/氯仿/异戊醇,剧烈振荡后,常温下13000g离心5分钟;4. 离心后,吸取上清于另一1.5ml eppendof管中,加入等体积氯仿,上下颠倒几次混匀后,常温下13000g离心5分钟;5. 吸取上清至另一eppendof管,加入1/10V3M NaAc(PH5.2)和2.5V预冷的无水乙醇,混匀,-20℃放置过夜以沉淀双链cDNA;6. 第二日,将昨日沉淀物在4℃,13000g离心60分钟以充分沉淀双链cDNA;7.离心完毕,弃上清,加入1ml 70%乙醇洗涤沉淀,常温下13000g离心5分钟;8.离心完毕,弃上清,干燥沉淀至无乙醇气味.注：第3，第4步可以用PCR 纯化试剂盒代替。PCR纯化试剂盒操作流程：1．溶液PE使用前应加入适量体积95％－100％的乙醇，混匀。2．向200ul二链补平产物中加入5倍体积的buffer PB，混匀。3．加入spin column中，13000rpm离心1min。4．加入0.75ml buffer PE，13000rpm离心1min。5．13000rpm，再离心1min。6．将spin column放入一新的离心管中，加入50ul buffer EB，静置10min。7．13000rpm离心2min。8．加入30ul buffer EB，静置10min。9．13000rpm离心2min。10．加入1/10体积3M的NaAc，2.5倍体积无水乙醇，混匀，－20℃沉淀过夜。
哪家公司的DNA提取试剂盒质量比较好?123
djls42017-11-09
效果好的话当然是promega或者omega的，不过会贵很多 都是美国佬的东西
便宜点的就是国产的tiangen，效果会差一点点 如果是大样本的全血 用这个也凑合
便宜点的就是国产的tiangen，效果会差一点点 如果是大样本的全血 用这个也凑合
Axygen 植物基因组DNA提取试剂盒说明书 123
文天羽丶両帥2017-09-29
axygen dna提取试剂盒有证书的，一般代理商都有证书，
这个是用于代理授权的，这个可以说是唯一辨别产品真伪的方法，
有证书的企业，销售的产品可信度比较高。
这个是用于代理授权的，这个可以说是唯一辨别产品真伪的方法，
有证书的企业，销售的产品可信度比较高。
大家能推荐好一点的DNA提取试剂盒吗 123
euniceyeh2009-03-30
:)各位战友，有没有谁用过北京五洲元业科贸的PCR基因组DNA模板快速制备试剂盒吗？！
有试剂A和B，传说10-30分钟就可以提取出来。不知道属不属实啊？
而且1000次580块钱！如果有用过的，可否跟我传授一下心得啊？
有试剂A和B，传说10-30分钟就可以提取出来。不知道属不属实啊？
而且1000次580块钱！如果有用过的，可否跟我传授一下心得啊？
【求助】请教 DOPPCR和ALUPCR在扩增BAC/PAC DNA制备探针...123
hansontcm2021-08-04
请教DOP-PCR和ALU-PCR在扩增BAC/PACDNA制备探针文库时的优劣，或者说都可以。
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