DNA Polymerase I (Pol I) catalyzes the incorporation of dNTPs into double-stranded DNA in a 5"→3" direction that is complementary to the DNA or RNAtemplate strand. It possesses 3"→5" exonuclease (proofreading) activity as well as 5"→3" exonuclease activity, making the enzyme useful for DNA end blunting and DNA labeling by nick translation. DNA Polymerase I is supplied in a buffer of 50 mM potassium phosphate (pH 6.5), 1 mM DTT and 50% glycerol.
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根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.
有试剂A和B,传说10-30分钟就可以提取出来。不知道属不属实啊?
而且1000次580块钱!如果有用过的,可否跟我传授一下心得啊?