RNA to cDNA EcoDry Premix is a convenient, lyophilized master mix that allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease. Each tube of premix contains reverse transcriptase and all the other components needed for cDNA synthesis. Simply add PCR-grade water along with your template, and you"re ready to go.
RNA to cDNA EcoDry Premix is a convenient, lyophilized master mix that allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease. Each tube of premix contains reverse transcriptase and all the other components needed for cDNA synthesis. Simply add PCR-grade water along with your template, and you"re ready to go.
A premium reverse transcriptase
RNA to cDNA EcoDry Premix features our high-performance, ultra-pure SMART MMLV Reverse Transcriptase. SMART MMLV reverse transcriptase allows the synthesis of high-quality first-strand cDNA of up to 11.7 kb, from virtually any transcript.
Convenient room-temperature storage
EcoDry Premix is ready to use. The simplified protocol has fewer steps, which saves you time and helps avoid pipetting errors. Additionally, the single-use design vastly reduces the risk of contamination. As with all EcoDry products, the premix can be stored at room temperature for months, without altering the performance of the reverse transcriptase or other components. This unprecedented convenience is especially useful in fieldwork, where access to refrigeration is limited.
Maximum flexibility and ease-of-use
RNA to cDNA EcoDry Premix is packaged individually, and sold in 8-tube strips with optically clear sealing caps. Use a single tube, the entire strip, or several strips for larger experiments. Each tube contains a lyophilized master mix complete with SMART MMLV reverse transcriptase, dNTPs, and your choice of oligo(dT)18 primers, random hexamer primers, or a combination of both.
Eco-friendly format
EcoDry lyophilized reverse transcription PCR premix is room temperature-stable. As a result it is shipped with minimal packaging, reducing your contribution of plastics and styrofoam to landfills. Additionally this eco-friendly format eliminates the need for refrigeration, further lowering environmental impact!
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这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
普通的手提或者试剂盒提取的土壤基因组DNA由于常常残留有PCR的强烈抑制物如腐殖酸、棕黄酸等杂质造成实验失败,此外,由于采用了剧烈的玻璃珠击打来破裂菌体,常常造成DNA剪切和降解。本公司经过长期研发开发出了具有自主知识产权的土壤基因组DNA,通过专利配方的腐殖酸和棕黄酸去除试剂配合特殊处理的纯化柱,可以最大程度的去除这些杂质,同时加上多次柱漂洗,确保得到的DNA具有极高纯度,此外独特的抽提和裂解体系可以迅速裂解细胞(壁)和灭活细胞内核酸酶,不需要借助玻璃珠破壁,有效保证了基因组DNA的完整性。
产品特点:
1.本公司独有的专利配方和纯化柱能有效去除腐殖酸等杂质。
2.不需要借助玻璃珠破壁,有效保证了基因组DNA的完整性,长度可达30kb -50kb,可直接用于PCR,Southern-blot和各种酶切反应。
3.兼容性强,适用于各种不同的土壤包括淤泥等提取困难的土壤。
4.多步骤去除各种杂质和抑制物,保证了极高纯度,OD260/OD280典型的比值达1.7~1.9。
5.不需要使用有毒的苯酚等试剂,也不需要乙醇沉淀等步骤。
6.快速,简捷,单个样品操作一般可在60分钟内完成。
你找下,杭州昊鑫生物
2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.

