![Anaspec/Neurogranin 28 - 43 [AAKIQASFRGHMARKK], Biotinylated - LC/5 mg/AS-27176](images/Anaspec/icon_msds.gif)
Product Name | Neurogranin 28 - 43 [AAKIQASFRGHMARKK], Biotinylated - LCBiotin - LC - AAKIQASFRGHMARKK |
Size | 5 mg |
Catalog # | AS-27176 |
US$ | $611 |
Purity | % Peak Area By HPLC ≥ 95% |
The native peptide AAKIQASFRGHMARKK (27173) is a synthetic PKC substrate derived from the sequence of neurogranin, a naturally occurring PKC substrate. The peptide is proven to be a PKC substrate with quite good specificity. It is phosphorylated by purified PKC with a Km of 150 nM. No significant phosphorylation of the peptide by either PKA or by CaMK 2 is reported. Substituting Arg36 with Ile causes a significant reduction in the affinity for PKC. Replacing Lys30 with Arg enhances the catalytic efficiency (Vmax/Km) for PKC but diminishes the selectivity of the substrate for PKC. It is generally considered that basic amino acids on both sides of the phosphorylated Ser are important structural determinants in PKC substrates. In addition, the presence of particular basic amino acids (Arg vs Lys) may also contribute to the degree of selectivity of a substrate for PKC. | |
Detailed Information | ![]() |
Storage | -20°C |
References | Ref: Chang, DK. et al. Biophys. J. 72, 554 (1997); Chen, SJ. et al. Biochem. 32, 1032 (1993). |
Molecular Weight | 2139.6 |
Biotin-LC-AAKIQASFRGHMARKK | |
Sequence(Three-Letter Code) | Biotin - LC - Ala - Ala - Lys - Ile - Gln - Ala - Ser - Phe - Arg - Gly - His - Met - Ala - Arg - Lys - Lys - OH |
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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
有试剂A和B,传说10-30分钟就可以提取出来。不知道属不属实啊?
而且1000次580块钱!如果有用过的,可否跟我传授一下心得啊?

