ProductHighlights:
TheNexteraRapidCaptureExomeandExpandedExomeareall-in-onekitsforlibrarypreparationandexomeenrichmentthatallowresearcherstoidentifycodingvariantsupto70%fasterthanothermethods.Thisoptimizedexomedelivers45Mbofexpertlyselectedexoniccontentandrequiresaslittleas4Gbofsequencing.
ExtendcoveragetoUTRsandmiRNAwithExpandedExome
NexteraRapidCaptureExpandedExomedelivers62Mbofgenomiccontent,includingexons,untranslatedregions(UTRs),andmiRNA.Thiskitfeaturesahighlyoptimizedprobesetthatdeliverscomprehensivecoverageofexonicsequence.
IlluminaprovidesexampledatasetstodemonstratethehighuniformityandaccuracyoftheNexteraRapidCaptureExomelibraryprepkit.Viewtheexomesequencingdata.
AddoncustomcontenttomakeNexteraRapidCaptureExomeevenmorepowerful
ResearcherscandefinecustomcontentforthisworkflowusingNexteraRapidCaptureCustomEnrichment.Thiscustomassayallowsresearcherstorapidlyinterrogatethoseportionsofthehumangenomemostimportanttotheirspecificresearch.Choosefromafullycustomdesignorstartfromadefinedcontentset,suchasNexteraRapidCaptureExome,andincludeareasofinterestusingadd-onfunctionality.LearnMore.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
AssayTime | 5hourstotalassaytime |
Hands-OnTime | 4hours |
InputQuantity | 1ugDNA(compatiblewithmostlargeDNAgenomes) |
MechanismofAction | Mechanicalfragmentation |
Multiplexing | Upto96dual-indexcombinationswithHT(high-throughput)kit |
VariantClass | SmallInsertions-Deletions(indels) |
SystemCompatibility | HiSeq2000,NextSeq550,HiSeq3000,NextSeq500,HiSeq2500,HiSeq4000 |
SpecializedSampleTypes | LowInput |
Technology | Sequencing |
SpeciesCategory | Human |
AutomationCapABIlity | LiquidHandlingRobots |
Method | ExomeSequencing |
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
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