
ProductHighlights:
FastLibraryPrep,OptimizedforSmallGenomes,PCRAmplicons,andPlasmids
- Rapidlibrarypreparation–completelibraryprepinaslittleas90minuteswithonly15minutesofhands-ontime
- Fasttimetoresults–gofromDNAtodatain8hourswithourbenchtopsequencers.
- Optimizedforsmallgenomes,PCRamplicons,andplasmids–onelibraryprepkitformanyapplications
- Innovativesamplenormalization–eliminatestheneedforlibraryquantificationbeforesamplepoolingandsequencing
WithNexteratechnology,DNAissimultaneouslyfragmentedandtaggedwithsequencingadaptersinasingletubeenzymaticreaction.NexteraXTsupportsultra-lowDNAinputofonly1ng.Itenablesawiderangeofinputsamples,including smallgenomes,PCRampliconsgreaterthan300bp,plasmids,microbialgenomes,concatenatedamplicons,anddouble-strandedCDNA.
Multiplexingofupto384samplesperNexteraXTlibraryisalsoavailableforthoseprojectsrequiringgreaterthroughput.Plus,thekitincludesaninnovativebead-basedsamplenormalizationthateliminatestheneedforlibraryquantificationpriortopoolingandsequencing.LibrariespreparedwithNexteraXTkitsarecompatIBLewithallIlluminasequencers.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatiblewiththislibrarypreparationkit
Specifications:
AssayTime | 90minutes |
Hands-OnTime | 15minutes |
MechanismofAction | EnzymaticFragmentation |
Multiplexing | Upto384uniquelyindexedsamplesmaybepooledandsequencedtogether. |
InputQuantity | 1ngDNA |
SpeciesCategory | Drosophila,AnySpecies,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria |
SystemCompatibility | MiSeq,NextSeq550,NextSeq500,MiSeqFGxinResearchMode |
Method | ShotgunSequencing,Whole-GenomeSequencing,DeNovoSequencing,16SrRNASequencing,AmpliconSequencing,TargetedDNASequencing |
VariantClass | SingleNucleotidePolymorphisms(SNPs),StructuralVariants |
SpecializedSampleTypes | LowInput,SingleCells |
Technology | Sequencing |
AutomationCapABIlity | LiquidHandlingRobots |
NexteraXTLibraryPreparationWorkflowimages/illumina/201710/nextera-xt-dna-library-prep-kit-web-graphic-1.png:
ebiomall.com






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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
各位战友好,因实验要求需要提取人的唾液DNA,但因标本需要在临床采集,无法做到当天就提取DNA,因此天根等漱口水提DNA的试剂盒都不满足实验需求。
之前一直在用艾德莱的唾液DNA保存运输提取试剂盒,但是这段时间下来感觉提取效率太低,而且特别不稳定,经常50%以上的标本都提不出来DNA,或者浓度特别低,完全崩溃的感觉!所以想更换试剂盒或者直接提供唾液DNA的保存液。另国外的这种试剂盒太贵了,因经费问题无法承受,所以希望战友们能够推荐一家效率高些的试剂盒,小女子不胜感激!!


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