ProductHighlights:
TruSeqExomeLibraryPrepKitisacost-effectivelibrarypreparationandexomeenrichmentsolutionwithindustry-leADIngaccuracy.Itoffers:
- Pre-enrichmentlibrarypoolingandoptimalcoverageforlow-costexomesequencing
- MechanicalshearingandTruSeqenrichmenttechnologythatyielduniformcoverageandgreaterthanorequalto80%on-targetsequencingreads
- Fullysupportedworkflowsolutiontosimplifyexomesequencing
Cost-EffectiveExomeSequencing
TheTruSeqExomeLibraryPrepKitsupports12-plexpre-enrichmentlibrarypooling,enablingresearcherstomaximizesequencingthroughputandidentifyvariantsinlesstimebysequencingupto12librariesperflowcelllane.Thehighon-targetpercentagerequiresfewersequencingcyclestoreachtheoptimallevelofcoverage,whilestillachievinghighcoverageuniformityforconfidentresults.Italsoenablessequencingofmoreexomesperrun,soresearcherscanmaximizetheirbudgets.
Tolearnmoreaboutcalculatingcoverageestimates,seethesequencingcoveragecalculator.
ProvenTruSeqDataQuality
TheTruSeqExomeLibraryPrepKitdelivers≥80%ofon-targetsequencingreadsforefficient,cost-effectiveexomesequencing.BycombiningtheTruSeqExomeLibraryPrepKitwithIlluminasystemsusingsequencingbysynthesis(SBS)technology,researcherscanidentifytruecodingvariantsandminimizefalse-positiveandfalse-negativecalls.
Convenient,IntegratedWorkflowSolution
Illuminaprovidesanintegrated,fullysupportedworkflowsolutionthatguidesresearchersfromlibrarypreparationthroughanalysis.Allcomponentsoftheexomesequencingworkflowaredesigned,optimized,andanalyticallyvalidatedtogether.ExpertIlluminascientistsprovideasinglesourceoftechnicalandfieldsupportforeverystepintheprocess.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
| AssayTime | 2.5days |
| Hands-OnTime | 6hours |
| InputQuantity | 100nggenomicDNA |
| ContentSpecifications | Capturescodingexons.Covers45Mbofexoniccontent(≥98%ofRefSeq,CCDS,andEnsemblcodingcontent). |
| MechanismofAction | Mechanicalfragmentation(COVARIS)andexomeenrichmentwithbiotinylatedcaptureprobes |
| Multiplexing | Upto12-plexenrichment |
| SystemCompatibility | NovaSeq5000,NextSeq550,HiSeq3000,NextSeq500,NovaSeq6000,HiSeq4000 |
| SpecializedSampleTypes | FFPE |
| Technology | Sequencing |
| SpeciesCategory | Human |
| AutomationCapABIlity | LiquidHandlingRobots |
| Method | ExomeSequencing |
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各位战友好，因实验要求需要提取人的唾液DNA，但因标本需要在临床采集，无法做到当天就提取DNA，因此天根等漱口水提DNA的试剂盒都不满足实验需求。
之前一直在用艾德莱的唾液DNA保存运输提取试剂盒，但是这段时间下来感觉提取效率太低，而且特别不稳定，经常50%以上的标本都提不出来DNA，或者浓度特别低，完全崩溃的感觉！所以想更换试剂盒或者直接提供唾液DNA的保存液。另国外的这种试剂盒太贵了，因经费问题无法承受，所以希望战友们能够推荐一家效率高些的试剂盒，小女子不胜感激！！
便宜点的就是国产的tiangen，效果会差一点点 如果是大样本的全血 用这个也凑合
根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家：普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家：深圳匹基,上海之江,中山达安,上海科华,上海复星.
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