ProductHighlights:
TruSeqExomeLibraryPrepKitisacost-effectivelibrarypreparationandexomeenrichmentsolutionwithindustry-leADIngaccuracy.Itoffers:
- Pre-enrichmentlibrarypoolingandoptimalcoverageforlow-costexomesequencing
- MechanicalshearingandTruSeqenrichmenttechnologythatyielduniformcoverageandgreaterthanorequalto80%on-targetsequencingreads
- Fullysupportedworkflowsolutiontosimplifyexomesequencing
Cost-EffectiveExomeSequencing
TheTruSeqExomeLibraryPrepKitsupports12-plexpre-enrichmentlibrarypooling,enablingresearcherstomaximizesequencingthroughputandidentifyvariantsinlesstimebysequencingupto12librariesperflowcelllane.Thehighon-targetpercentagerequiresfewersequencingcyclestoreachtheoptimallevelofcoverage,whilestillachievinghighcoverageuniformityforconfidentresults.Italsoenablessequencingofmoreexomesperrun,soresearcherscanmaximizetheirbudgets.
Tolearnmoreaboutcalculatingcoverageestimates,seethesequencingcoveragecalculator.
ProvenTruSeqDataQuality
TheTruSeqExomeLibraryPrepKitdelivers≥80%ofon-targetsequencingreadsforefficient,cost-effectiveexomesequencing.BycombiningtheTruSeqExomeLibraryPrepKitwithIlluminasystemsusingsequencingbysynthesis(SBS)technology,researcherscanidentifytruecodingvariantsandminimizefalse-positiveandfalse-negativecalls.
Convenient,IntegratedWorkflowSolution
Illuminaprovidesanintegrated,fullysupportedworkflowsolutionthatguidesresearchersfromlibrarypreparationthroughanalysis.Allcomponentsoftheexomesequencingworkflowaredesigned,optimized,andanalyticallyvalidatedtogether.ExpertIlluminascientistsprovideasinglesourceoftechnicalandfieldsupportforeverystepintheprocess.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
AssayTime | 2.5days |
Hands-OnTime | 6hours |
InputQuantity | 100nggenomicDNA |
ContentSpecifications | Capturescodingexons.Covers45Mbofexoniccontent(≥98%ofRefSeq,CCDS,andEnsemblcodingcontent). |
MechanismofAction | Mechanicalfragmentation(COVARIS)andexomeenrichmentwithbiotinylatedcaptureprobes |
Multiplexing | Upto12-plexenrichment |
SystemCompatibility | NovaSeq5000,NextSeq550,HiSeq3000,NextSeq500,NovaSeq6000,HiSeq4000 |
SpecializedSampleTypes | FFPE |
Technology | Sequencing |
SpeciesCategory | Human |
AutomationCapABIlity | LiquidHandlingRobots |
Method | ExomeSequencing |
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
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