ProductHighlights:
TruSeqExomeLibraryPrepKitisacost-effectivelibrarypreparationandexomeenrichmentsolutionwithindustry-leADIngaccuracy.Itoffers:
- Pre-enrichmentlibrarypoolingandoptimalcoverageforlow-costexomesequencing
- MechanicalshearingandTruSeqenrichmenttechnologythatyielduniformcoverageandgreaterthanorequalto80%on-targetsequencingreads
- Fullysupportedworkflowsolutiontosimplifyexomesequencing
Cost-EffectiveExomeSequencing
TheTruSeqExomeLibraryPrepKitsupports12-plexpre-enrichmentlibrarypooling,enablingresearcherstomaximizesequencingthroughputandidentifyvariantsinlesstimebysequencingupto12librariesperflowcelllane.Thehighon-targetpercentagerequiresfewersequencingcyclestoreachtheoptimallevelofcoverage,whilestillachievinghighcoverageuniformityforconfidentresults.Italsoenablessequencingofmoreexomesperrun,soresearcherscanmaximizetheirbudgets.
Tolearnmoreaboutcalculatingcoverageestimates,seethesequencingcoveragecalculator.
ProvenTruSeqDataQuality
TheTruSeqExomeLibraryPrepKitdelivers≥80%ofon-targetsequencingreadsforefficient,cost-effectiveexomesequencing.BycombiningtheTruSeqExomeLibraryPrepKitwithIlluminasystemsusingsequencingbysynthesis(SBS)technology,researcherscanidentifytruecodingvariantsandminimizefalse-positiveandfalse-negativecalls.
Convenient,IntegratedWorkflowSolution
Illuminaprovidesanintegrated,fullysupportedworkflowsolutionthatguidesresearchersfromlibrarypreparationthroughanalysis.Allcomponentsoftheexomesequencingworkflowaredesigned,optimized,andanalyticallyvalidatedtogether.ExpertIlluminascientistsprovideasinglesourceoftechnicalandfieldsupportforeverystepintheprocess.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
| AssayTime | 2.5days |
| Hands-OnTime | 6hours |
| InputQuantity | 100nggenomicDNA |
| ContentSpecifications | Capturescodingexons.Covers45Mbofexoniccontent(≥98%ofRefSeq,CCDS,andEnsemblcodingcontent). |
| MechanismofAction | Mechanicalfragmentation(COVARIS)andexomeenrichmentwithbiotinylatedcaptureprobes |
| Multiplexing | Upto12-plexenrichment |
| SystemCompatibility | NovaSeq5000,NextSeq550,HiSeq3000,NextSeq500,NovaSeq6000,HiSeq4000 |
| SpecializedSampleTypes | FFPE |
| Technology | Sequencing |
| SpeciesCategory | Human |
| AutomationCapABIlity | LiquidHandlingRobots |
| Method | ExomeSequencing |
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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
普通的手提或者试剂盒提取的土壤基因组DNA由于常常残留有PCR的强烈抑制物如腐殖酸、棕黄酸等杂质造成实验失败,此外,由于采用了剧烈的玻璃珠击打来破裂菌体,常常造成DNA剪切和降解。本公司经过长期研发开发出了具有自主知识产权的土壤基因组DNA,通过专利配方的腐殖酸和棕黄酸去除试剂配合特殊处理的纯化柱,可以最大程度的去除这些杂质,同时加上多次柱漂洗,确保得到的DNA具有极高纯度,此外独特的抽提和裂解体系可以迅速裂解细胞(壁)和灭活细胞内核酸酶,不需要借助玻璃珠破壁,有效保证了基因组DNA的完整性。
产品特点:
1.本公司独有的专利配方和纯化柱能有效去除腐殖酸等杂质。
2.不需要借助玻璃珠破壁,有效保证了基因组DNA的完整性,长度可达30kb -50kb,可直接用于PCR,Southern-blot和各种酶切反应。
3.兼容性强,适用于各种不同的土壤包括淤泥等提取困难的土壤。
4.多步骤去除各种杂质和抑制物,保证了极高纯度,OD260/OD280典型的比值达1.7~1.9。
5.不需要使用有毒的苯酚等试剂,也不需要乙醇沉淀等步骤。
6.快速,简捷,单个样品操作一般可在60分钟内完成。
你找下,杭州昊鑫生物

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