ProductHighlights:
TheTruSeqRapidExomeLibraryPrepKitdeliversasimple,efficientmethodforhigh-confidencecallingofexonicvariants.Thefastlibrarypreparationandexomeenrichmentworkflowdeliverlibrariesin1daywithnoneedforadditionalequipment,givinglabstheflexibilitytoplanprojectsaccordingtosamplevolume.Thiskitoffers:
- Enhancedtransposomechemistryresultsinreducedbias,providingconsistentlibrarypreparationandexomeenrichment.
- Rapid,automation-friendlyworkflowcompletesin1daywithonly3hoursofhands-ontime.
- Highcoverageuniformityacrossexonicregionsandon-target%
ProvenTruSeqDataQuality
TheTruSeqRapidExomeLibraryPrepKitdeliversanaverageof75%ofon-targetsequencingreads.Thishighon-targetpercentagerequiresfewersequencingcyclestoreachdesiredcoveragelevels,butstillachievesuniformcoverageforhigh-confidenceresults.
Tolearnmoreaboutcalculatingcoverageestimates,seethesequencingcoveragecalculator.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
AssayTime | 1day |
Hands-OnTime | 3hours |
InputQuantity | 50nggenomicDNA |
ContentSpecifications | Capturescodingexons.Covers45Mbofexoniccontent(≥98%ofRefSeq,CCDS,andEnsemblcodingcontent). |
MechanismofAction | Transposase-basedfragmentationandexomeenrichmentwithbiotinylatedcaptureprobes |
Multiplexing | Upto12-plexenrichment |
Technology | Sequencing |
SpeciesCategory | Human |
AutomationCapABIlity | LiquidHandlingRobots |
SystemCompatibility | NextSeq500,NovaSeq6000,HiSeq4000 |
Method | ExomeSequencing |
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
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