ProductHighlights:
TheTruSeqRapidExomeLibraryPrepKitdeliversasimple,efficientmethodforhigh-confidencecallingofexonicvariants.Thefastlibrarypreparationandexomeenrichmentworkflowdeliverlibrariesin1daywithnoneedforadditionalequipment,givinglabstheflexibilitytoplanprojectsaccordingtosamplevolume.Thiskitoffers:
- Enhancedtransposomechemistryresultsinreducedbias,providingconsistentlibrarypreparationandexomeenrichment.
- Rapid,automation-friendlyworkflowcompletesin1daywithonly3hoursofhands-ontime.
- Highcoverageuniformityacrossexonicregionsandon-target%
ProvenTruSeqDataQuality
TheTruSeqRapidExomeLibraryPrepKitdeliversanaverageof75%ofon-targetsequencingreads.Thishighon-targetpercentagerequiresfewersequencingcyclestoreachdesiredcoveragelevels,butstillachievesuniformcoverageforhigh-confidenceresults.
Tolearnmoreaboutcalculatingcoverageestimates,seethesequencingcoveragecalculator.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
| AssayTime | 1day |
| Hands-OnTime | 3hours |
| InputQuantity | 50nggenomicDNA |
| ContentSpecifications | Capturescodingexons.Covers45Mbofexoniccontent(≥98%ofRefSeq,CCDS,andEnsemblcodingcontent). |
| MechanismofAction | Transposase-basedfragmentationandexomeenrichmentwithbiotinylatedcaptureprobes |
| Multiplexing | Upto12-plexenrichment |
| Technology | Sequencing |
| SpeciesCategory | Human |
| AutomationCapABIlity | LiquidHandlingRobots |
| SystemCompatibility | NextSeq500,NovaSeq6000,HiSeq4000 |
| Method | ExomeSequencing |
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各位战友好,因实验要求需要提取人的唾液DNA,但因标本需要在临床采集,无法做到当天就提取DNA,因此天根等漱口水提DNA的试剂盒都不满足实验需求。
之前一直在用艾德莱的唾液DNA保存运输提取试剂盒,但是这段时间下来感觉提取效率太低,而且特别不稳定,经常50%以上的标本都提不出来DNA,或者浓度特别低,完全崩溃的感觉!所以想更换试剂盒或者直接提供唾液DNA的保存液。另国外的这种试剂盒太贵了,因经费问题无法承受,所以希望战友们能够推荐一家效率高些的试剂盒,小女子不胜感激!!
便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.

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