
ProductHighlights:
TheTruSeqRapidExomeLibraryPrepKitdeliversasimple,efficientmethodforhigh-confidencecallingofexonicvariants.Thefastlibrarypreparationandexomeenrichmentworkflowdeliverlibrariesin1daywithnoneedforadditionalequipment,givinglabstheflexibilitytoplanprojectsaccordingtosamplevolume.Thiskitoffers:
- Enhancedtransposomechemistryresultsinreducedbias,providingconsistentlibrarypreparationandexomeenrichment.
- Rapid,automation-friendlyworkflowcompletesin1daywithonly3hoursofhands-ontime.
- Highcoverageuniformityacrossexonicregionsandon-target%
ProvenTruSeqDataQuality
TheTruSeqRapidExomeLibraryPrepKitdeliversanaverageof75%ofon-targetsequencingreads.Thishighon-targetpercentagerequiresfewersequencingcyclestoreachdesiredcoveragelevels,butstillachievesuniformcoverageforhigh-confidenceresults.
Tolearnmoreaboutcalculatingcoverageestimates,seethesequencingcoveragecalculator.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
AssayTime | 1day |
Hands-OnTime | 3hours |
InputQuantity | 50nggenomicDNA |
ContentSpecifications | Capturescodingexons.Covers45Mbofexoniccontent(≥98%ofRefSeq,CCDS,andEnsemblcodingcontent). |
MechanismofAction | Transposase-basedfragmentationandexomeenrichmentwithbiotinylatedcaptureprobes |
Multiplexing | Upto12-plexenrichment |
Technology | Sequencing |
SpeciesCategory | Human |
AutomationCapABIlity | LiquidHandlingRobots |
SystemCompatibility | NextSeq500,NovaSeq6000,HiSeq4000 |
Method | ExomeSequencing |
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我分别用3ul、5ul、8ul都试过了,没有任何条带,用别人别的方法提好的DNA做,却能出现结果,不知道是自己提取的DNA浓度不够,还是根本没有提取出来。
不知道谁用过,或者有更好的全血提取NDA的试剂盒推荐呢?
本人刚刚开始做PCR,谢谢各位高手!
有试剂A和B,传说10-30分钟就可以提取出来。不知道属不属实啊?
而且1000次580块钱!如果有用过的,可否跟我传授一下心得啊?
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。


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