
ProductHighlights:
TruSeqSmallRNALibraryPreparationKitsprovidereagentstogeneratesmallRNAlibrariesdirectlyfromtotalRNA.MicroRNAs(miRNAs)generatedbyDicerprocessingareefficientlytargetedbytheincludedmodifiedadapters.
Thesekitsenablemultiplexedsequencingwiththeintroductionof48uniqueindexes,allowingmiRNAandsmallRNAdiscoveryandprofilingthroughputtomatchtheunparalleledoutputofIlluminasequencing.Indexesareaddedinauniversalamplificationreaction,greatlyreducingligationbiasandensuringaccuratemeasurementofmiRNAexpression.
Workflowimprovementsenablestreamlinedsamplepreparation,allowingeconomicalstudiescoveringallsmallRNAtranscriptsinanyspecies.CompatIBLeapplicationsincludefindingnovelmiRNAs,characterizingvariationsuchasisomerswithsingle-baseresolution,andanalyzingdifferentialexpressionwithoutpriorassumptions.
TruSeqSmallRNASampleDatasets
TheHumanBrainReferenceRNA(HBRR)andUniversalHumanReferenceRNA(UHRR)sampleswerepreparedusingtheTruSeqSmallRNALibraryPrepKit.TheselibrariesweresequencedontheMiniSeqSystemusingtheMiniSeqHighOutputReagentKitata1x36bpreadlengthconfiguration.Thetotalyieldwas0.91Gbwith97%ofbasesatoraboveQ30.
BrowsetheDataInBaseSpaceSequenceHub:
- ViewRun(primaryanalysisandmetrics)
- ViewProject(secondaryanalysiswithBaseSpaceApps)
Specifications:
AssayTime | ~10.5hours |
Hands-OnTime | ~30minutes |
InputQuantity | 25to100ngtotalRNAfromspecieswithpolyAtails |
ContentSpecifications | Capturesthecodingtranscriptomewithstrandinformation |
MechanismofAction | Oligo-dTbeadscapturepolyAtails |
Multiplexing | Upto24-plexpoolingwithadditionalNeoPrepruns |
SampleThroughput | Upto16samplesperNeoPreplibrarycard |
SpeciesCategory | Drosophila,AnySpecies,Mouse,Zebrafish,Human,Rat,Plant,Nematode |
SystemCompatibility | MiSeq,NextSeq550,MiniSeq,NextSeq500 |
SpecializedSampleTypes | FFPE,LowInput |
Technology | Sequencing |
AutomationCapABIlity | LiquidHandlingRobots |
VariantClass | NovelTranscripts,TranscriptVariants |
Method | miRNAandSmallRNASequencing |
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
有试剂A和B,传说10-30分钟就可以提取出来。不知道属不属实啊?
而且1000次580块钱!如果有用过的,可否跟我传授一下心得啊?


暂无品牌分类