ProductHighlights:
High-QualityWhole-TranscriptomeAnalysiswithPreciseStrandInformation
RNA-Seqtechnologyprovidesuniformcoverage,precisemeasurementofstrandorientationandhigh-confidencediscoveryoffeaturessuchasalternativetranscripts,antisenseexpressionandallele-specificexpressionacrossbothcodingandmultipleformsofnoncodingRNA.TheTruSeqStrandedTotalRNAwithRibo-ZeroPlantKitcouplesthebenefitsofTruSeqRNAlibrarypreparationwithRibo-ZeroribosomalRNAreductionchemistry,providingarobustandscalablesolutionforwhole-transcriptomeanalysis.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
EfficientRibosomalRNAReductionAcrossaRangeofPlantSpecies,StudyDesigns
TheTruSeqStrandedTotalRNAwithRibo-ZeroPlantKitenablesrapidandspecificremovalofcytoplasmic,mitochondrialandchloroplastribosomalRNAfromleaf,seed,androottissue.Thekithasbeenvalidatedforuseinmultipleplantspecies,includingArABIdopsisthaliana,rice,andmaize,andwillprovideefficientrRNAremovalinabroadrangeofadditionalspecies.*
*Forinformationonaparticularspeciesofinterest,contactIlluminaTechnicalSupport.
Specifications:
| AssayTime | ~6.5hours |
| Hands-OnTime | ~3.5hours |
| InputQuantity | 10nghigh-qualitygenomicDNA;10–100ngFFPEDNA(dependingonQCresults) |
| MechanismofAction | Probehybridization,extension-ligation,andPCR |
| Multiplexing | 1–96 |
| ContentSpecifications | Designcustomprobestosequencegenomicregionsofinterest.Contentrange:4–650kb |
| VariantClass | ShortTandemRepeats(STRs),SingleNucleotidePolymorphisms(SNPs),GeneFusions,LossofHeterozygosity(LOH),SomaticVariants,ChromosomalAbnormalities,GermlineVariants,StructuralVariants,Insertions-Deletions(indels) |
| SystemCompatibility | HiSeq2000,MiSeq,NextSeq550,HiSeq3000,HiSeq1000,MiSeqDxinResearchMode,MiniSeq,HiSeq1500,NextSeq500,MiSeqFGxinResearchMode,HiSeq2500,HiSeq4000 |
| SpeciesCategory | Other,Bovine,Mouse,Human,Rat |
| SpecializedSampleTypes | FFPE,LowInput |
| Technology | Sequencing |
| AutomationCapability | LiquidHandlingRobots |
| Method | AmpliconSequencing,CustomSequencing |
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各位战友好,因实验要求需要提取人的唾液DNA,但因标本需要在临床采集,无法做到当天就提取DNA,因此天根等漱口水提DNA的试剂盒都不满足实验需求。
之前一直在用艾德莱的唾液DNA保存运输提取试剂盒,但是这段时间下来感觉提取效率太低,而且特别不稳定,经常50%以上的标本都提不出来DNA,或者浓度特别低,完全崩溃的感觉!所以想更换试剂盒或者直接提供唾液DNA的保存液。另国外的这种试剂盒太贵了,因经费问题无法承受,所以希望战友们能够推荐一家效率高些的试剂盒,小女子不胜感激!!
根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.

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