ProductHighlights:
TheTruSeqStrandedmRNALibraryPrepKitforNeoPrepoffersastreamlined,cost-efficient,andscalablesolutionforcodingtranscriptomeanalysis.Itenablesrobustinterrogationofbothstandardandlow-qualitysamples.
- Simplifiedandseamlessworkflowsolution–Generatelibrarieswithminimalhands-ontime
- Unparalleledperformanceandreproducibility–InterrogategeneexpressionwithTruSeqStrandedmRNAcoverageandquality,andreduceduservariABIlity
- Lowinputrequirement–Beginlibraryprepwithaslittleas25ngtotalRNA
PreciseandAccurate
GetprecisemeasurementofmRNAstrandorientationfordetectionofantisensetranscription,enhancedtranscriptannotation,andincreasedalignmentefficiency.Highcoverageuniformityenhancesthediscoveryoffeaturessuchasalternativetranscripts,genefusions,andallele-specificexpression.
Cost-Efficient
Strandedinformationidentifiesfromwhichofthe2DNAstrandsagivenRNAtranscriptwasderived.Thisinformationprovidesincreasedconfidenceintranscriptannotation,particularlyfornonhumansamples.Identifyingstrandoriginincreasesthepercentageofreadsthatalign,reducingsequencingcostspersample.
NeoPrepSystemenhancesTruSeqStrandedmRNAperformance
TheNeoPrepSystemenhancesTruSeqStrandedmRNAperformancebyprovidinghigh-qualityreproducIBLeresults,evenwithlowinputamountsofRNA.Digitalmicrofluidicstechnologypreciselymanipulatesdropletsthatperformthelibraryprepworkflow,withinthetightlycontrolledenvironmentoftheNeoPreplibrarycard.
Asimple,intuitiveworkflowdelivers16libraries,eliminatingmostmanualsteps,andreducinghands-ontimefrom~4.5hourstojust30minutes.Inaddition,digitalmicrofluidicsrequireslessRNAinput,enablingexcellentperformancefromaslittleas25ngoftotalRNA.
Specifications:
AssayTime | ~10.5hours |
Hands-OnTime | ~30minutes |
InputQuantity | 25to100ngtotalRNAfromspecieswithpolyAtails |
ContentSpecifications | Capturesthecodingtranscriptomewithstrandinformation |
MechanismofAction | Oligo-dTbeadscapturepolyAtails |
Multiplexing | Upto24-plexpoolingwithadditionalNeoPrepruns |
SampleThroughput | Upto16samplesperNeoPreplibrarycard |
SystemCompatibility | GenomeAnalyzerIIx,HiSeq2000,MiSeq,NextSeq550,HiSeq3000,HiSeq1000,NeoPrep,HiSeq1500,NextSeq500,HiSeq2500,HiSeq4000 |
SpeciesCategory | Other,Mammalian,Bovine,Mouse,Human,Rat |
VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,NovelTranscripts,TranscriptVariants |
Technology | Sequencing |
Method | mRNASequencing |
AutomationCapability | NeoPrepDigitalMicrofluidics |
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根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.
有试剂A和B,传说10-30分钟就可以提取出来。不知道属不属实啊?
而且1000次580块钱!如果有用过的,可否跟我传授一下心得啊?
暂无品牌分类