
Description:
PCR-FreeKAPAHyperPlusKits
NGSlibrarypreparation. Evolved.
TheKAPAHyperPluskitprovidesastreamlinedworkflowthatincludesfragmentationandlibrarypreparationinasingletube.Buildingonindustry-leADInglibraryconstructionefficiencies,thisintegratedsolutioncombinesenzymaticfragmentation,similarinqualitytomechanicalshearing,withthespeedandconvenienceoftagmentation-basedworkflows.Benefitsinclude:
- DNAfragmentationandlibraryprepin2.5hours*
- FlexIBLeDNAsampleinputfrom1ng–1µg*
- Reducedbiasandmaximizesequencecoverage*
- PCR-freeworkflows
NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.
Downloadour AdapterandBeadCalculator
*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

KAPAHyperPlusWorkflow
ProductHighlights
Tunableandreproduciblefragmentation
- Adjustlibraryinsertsizesfrom150–800bpbyvaryingfragmentationtime
- ReproducibleinsertsizesacrossarangeofGCcontentandDNAinputamounts

Industryleadinglibraryyields…
- Routinelyachieveconversionrates>80%from≥100nginput*
- SuperiorperformanceacrossarangeofDNAinputamounts*
- HighlibraryyieldsenablePCR-freeworkflowsfromaslittleas50ngstartingmaterial*
…Enablesuperiorsequencingresults
- Higherconversionratesresultsinfeweramplificationcyclesandlowerduplicationrates
- Detectlow-frequencymutationswithhighconfidenceduetogreaterlibrarydiversityandmoreuniformsequencecoverage
Minimalsequencecoveragebias
- Lowersequencebiaswhencomparedtotagmentationandotherenzymaticfragmentationmethods*
- Equivalentperformancetomechanicalshearing*
- Lessbiasleadstomoreuniformsequencingcoverageandreducedsequencingcosts*
*Dataonfile.
RelatedProducts
Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPAhgDNAQuantificationandQCKit

KAPALibraryAmplificationKits

KAPALibraryQuantificationKits

KAPAStrandedmRNA-SeqKits
Applications:
Applications- WholeGenomeSequencing
- WholeExomeSequencing
- TargetedSequencing(custompanels)
- FFPESampleSequencing
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageKitscanbestoredforupto6monthsat-20ºC.
KitscontainKAPAFragEnzyme,KAPAFragBuffer(10X),KAPAFragConditioningSolution,KAPAEndRepair&A-TailingBuffer, KAPAEndRepair&A-TailingEnzyme, KAPALigationBuffer, KAPADNALigase, KAPAHiFiHotStartReadyMixand KAPALibraryAmplificationPrimerMix.
Specifications
- SpecDescription
- CompatiblePlatformlluminaHiSeq®,MiSeq®,NextSeq®
- LibraryTypeDNA
- StartingMaterialgenomicDNA,FFPEDNA,PCRamplicons
- InputAmount1ng–1µg
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
我分别用3ul、5ul、8ul都试过了,没有任何条带,用别人别的方法提好的DNA做,却能出现结果,不知道是自己提取的DNA浓度不够,还是根本没有提取出来。
不知道谁用过,或者有更好的全血提取NDA的试剂盒推荐呢?
本人刚刚开始做PCR,谢谢各位高手!
各位战友好,因实验要求需要提取人的唾液DNA,但因标本需要在临床采集,无法做到当天就提取DNA,因此天根等漱口水提DNA的试剂盒都不满足实验需求。
之前一直在用艾德莱的唾液DNA保存运输提取试剂盒,但是这段时间下来感觉提取效率太低,而且特别不稳定,经常50%以上的标本都提不出来DNA,或者浓度特别低,完全崩溃的感觉!所以想更换试剂盒或者直接提供唾液DNA的保存液。另国外的这种试剂盒太贵了,因经费问题无法承受,所以希望战友们能够推荐一家效率高些的试剂盒,小女子不胜感激!!
便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合

