2018年开年后我们发现Thermo官网的qubit3.0居然下架了，此前的17年10月份行业内就在传Qubit3.0已经停产

与此同时，Qubit4.0强势推出

通过产品图片我们发现Qubit3.0与Qubit4.0外观也存在一些差异，不过Qubit4.0却比3.0贵了整整600美金

具体有哪些差异呢？

目前只能通过官网信息看到一点端倪

Qubit4.0的货号是q33226

TheQubit4FlurometeristhelatestversionofthepopularQubitfluorometerdesignedtoaccuratelymeasureDNA,RNA,andproteinquantity,andnowalsoRNAintegrityandquality,usingthehighlysensitiveQubitassays.TheQubit4Fluorometerwasre-engineeredtoruntheQubitRNAIQ(integrity&quality)assay.TheQubit4FluorometerandRNAIQAssayKitworktogethertoaccuratelydistinguishintactfromdegradedRNAinjusttwoeasysteps.

ForallQubitassays,theconcentrationorqualityofthetargetmoleculeinthesampleisreportedbyafluorescentdyethatemitsasignalonlywhenboundtothetarget,whichminimizestheeffectsofcontaminantsontheresult.Theeasy-to-usetouch-screenmenusmakeiteasytoselectandruntheassaysyouneed,withresultsdisplayedinjustafewseconds.

KeyfeaturesoftheQubit4Fluorometerinclude:
•FastandhighlyaccuratequantitationofDNA,RNA,andproteininlessthanthreesecondspersample
•MeasuresintactRNAinlessthan5secondspersample
•Highlevelsofaccuracyusingonly1–20μLofsample,evenwithverydilutesamples
•On-boardReagentCalculatorquicklygeneratesQubitworkingsolutionpreparationinstructions
•Storesresultsfromupto1000samples
•Large5.7-inch,state-of-the-artcolortouchscreenforeasyworkflownavigation
•Graphicaldisplayindicateswhensamplesareintheextendedrangeoroutofrange
•Smallfootprintsavesspaceonyourbench
•ExportsdatatoaUSBdriveordirectlytoyourcomputerviaaUSBcable
•ABIlitytopersonalizeyourQubitfluorometerwiththeassaysyourunmost,addnewassays,orevencreateyourownassayswiththeMyQubitsoftwareandwebtool
•LanguageofyourchoiceincludingEnglish,French,Spanish,Italian,German,simplifiedChinese,andJapanese

TheQubit4FlurometercanalsobeusedtodirectlymeasurethefluorescenceofsamplesusingFluorometermode.Additionally,IonSphereParticlequalitycanbeevaluatedontheQubit4FluorometerusingtheIonSphereQualityControlKitpriortoperformingasequencingrunontheIonPGMsequencer.

EnablesmoreaccurateresultsthanwithUVabsorbanceatlowconcentrations
TheQubit4FluorometerisfarmoresensitivethanUVabsorbance,whichmeasuresanythingabsorbingat260nm,includingDNA,RNA,protein,freenucleotides,orexcesssalts.Moreover,UVspectrophotometryisoftennotsensitiveenoughtoaccuratelymeasurelowconcentrationsofDNAandRNA.WiththeQubitfluorometer,yourresearchisenhancedbymoreaccuratemeasurementsbecausethedyesintheQubitassaykitsfluoresceonlywhenboundtotheselectedmolecule(DNA,RNAorprotein)inyoursample.Thisallowsyoutoavoidrepeatingworkduetoinaccuratemeasurements.

Fast,simple,andaccuratemeasurementofRNAquality
TheQubitRNAIQassayprovidesafast,simplemethodtocheckwhetheranRNAsamplehasdegraded.Theassayutilizestwouniquedyes:onethatbindstolarge,intactRNA,andanotherthatselectivelybindstosmall,degradedRNA.TogethertheyenableyoutoquicklyassessthequalityandintegrityofanRNAsample.Touse,simplyaddyoursamplestotheRNAIQworkingsolution,thenmeasureontheQubit4Fluorometer.

Savesamplesandbenchspace
ThethoughtfullydesignedQubit4Fluorometerrequiressamplesofonly1–20µL.Itisintendedforuseatroomtemperatureandoccupiesonlyasmallamountofbenchspace.TheQubit4Fluorometerfeaturesadvancedopticsanddataanalysisalgorithms,andcomesequippedwithaUSBthumbdriveandcablefordatatransfertoExcel™softwareandforsoftwaredownloads,aswellasauniversalpowersupply,fourplugadapters,andelectromagneticCEcertification.

Singlesamplemeasurementsareabreeze
Thisuser-friendlyfluorometeriscombinedwithquickandsimpleassayprocedures.Calculationsandsettingsareautomaticallyperformedbytheinstrument.TheQubitassaysforusewiththeQubit4Fluorometerareallperformedusingthesamegeneralprotocol,whichusesasimplemix-and-readformatwithincubationtimesofonlytwominutesfortheDNAandRNAassays.500Lthin-walledPCRtubes(Cat.No.Q32856)arerequired.

Systemspecificationsinclude:

Instrumentdimensions:5.4"(w)x10"(l)x2.2"(h)(13.6cmx25cmx5.5cm)
Weight:743g
Dynamicrange:5ordersofmagnitude
Processingtime:≤seconds/sample
Lightsources:blueLED(max~470nm),redLED(max~635nm)
Excitationfilters:blue(430–495nm),red(600–645nm)
Emissionfilters:green(510–580nm),red(665–720nm)
Detectors:photodiodes,measurementcapabilityfrom300–1,000nm
Warm-uptime:<35seconds
USBthumbdrive:4GB

对比一下QUBIT3.0的说明如下：

Qubit®3.0荧光定量仪为全新升级的台式荧光计，配套高灵敏的Qubit®定量分析试剂盒，精确定量DNA,RNA和蛋白质浓度。采用专门研制的荧光染料，只有与样品中的靶分子特异结合时方可发射荧光信号，从而报告靶分子的浓度，因此这种特异性可以使您获得比传统紫外吸光法更加精确的结果。

Qubit®3.0荧光定量仪主要特点包括：

• 强大的双核处理器，5秒内快速准确地定量DNA,RNA和蛋白

• 最低检测1μL样品（10pg/μLDNA或12.5μg/mL蛋白）

• 最多储存1,000个样品结果，可通过U盘导出或直接与电脑连接

• 5.7英寸大尺寸彩色触摸屏，直观的导航按钮

• 个性化设置常规使用的应用，添加新的应用，甚至通过MyQubit自定义创建新应用

• 灵活选择操作语言，包括英语，简体中文，法语，西班牙语，德语，意大利语及日语