MakeyourtargetspecificgRNAlentivectorswithdesiredMarkers!
pLenti-gRNA-U6/H1- CloningKitisdesignedfor cloningofdouble-strandDNAencodingyourdesired gRNAsequenceforCRISPRgenomicgeneediting.Oncetranscribed,thetargetspecificgRNAwillguildCAS9enzymetocutthegenomicDNAatdesiredloci.
TomakeyourtargetspecificgRNAlentivector,twosyntheticgRNAoligonucleotidesare firstannealedtoformthedoublestandDNAduplexwhichis thenclonedintotheprovided,linearpLenti-gRNA-U6 /H1lentivectorviaT4enzymeligation.
ProductFeatures:
1)Flexibility toselectdesiredpromoter: H1orU6;
2)Flexibilitytochoose desired selectionmarker:antibioticorantibiotic-fluorescentfusiondualmarker;
3)Easyandhighefficiencycloningreaction:withprovidedpre-cut,readytouse,lineargRNAlentivector,youdoNOTneedthetedious,yetoftentroublesomelentivectorbackbonepreparation.TheCloningpositiveratecloseto100%.
4)Everythingisincludedinthiskit,enoughforgenerating10gRNAlentivectors;
5)OptionallyusedastetracyclineinducIBLe gRNAtranscription(forH1promoteronly);
ThisproductisthelentivectorversionhavingthegRNAdrivenbyaconstitutiveanoptionalinducible humanH1 promoter witha GFP-Blasticidin dualselectionmarkerunderRsvpromoter.PleaseseeProductManualfordetails.
Kitcontents:
pLenti-H1-(GFP-Bsd)linearvector: 10ul(10rxn,1ul/rxn)
10xgRNAoligoannealingsolution: 25ul
10Xligationbuffer: 20ul
T4ligaseenzyme: 10ul(10rxn,1ul/rxn)
Cloningcontrolinsert:annealedNeg-gRNAduplex: 10 ul(10rxn,1ul/rxn)
Sequencingprimer: 10ul(10xn,1ul/rxn)DH5achemicalcompetentcells: 10vials(10xn,1vial/rxn)
ThegeneratedgRNAlentivectorcanbeusedfortransfectionorelectroporationbasedCRISPRediting,orusedforgRNAlentivirusproductionandthenusethegRNAlentivirusfor CRISPRediting.
Cat#: gRNA-H1-GB
ebiomall.com
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请各位大侠帮忙分析一下原因！！万分感谢！！！
ClonExpress II One Step Cloning Kit同源重组克隆试剂盒同原理重组效率高我实验室都用克隆试剂盒基本用重复实验功推荐使用产品
您线粒体蛋白提取试剂盒产品特点：1.离吸附柱内硅基质膜全部采用进口世界著名公司特制吸附膜柱与柱间吸附量差异极重复性克服产试剂盒膜质量稳定弊端
2.使用优质溶胶液含传统溶胶液碘化钠高氯酸盐抑制收酶切、连接克隆等游反应
3.独特溶胶液/结合液配溶胶结合两种功能统试剂盒运用于琼脂糖DNA收、PCR产物清洁纯化、酶切产物纯化收等种情况节省需购买种试剂盒费用
4.溶胶液/结合液调制黄颜色便于观察溶胶效监测pH值变化达佳结合效提高收效率
5.改进溶胶液配,提高缓冲能力稳定性即使品变化能PH缓冲佳结合范围内
6.快速、便需要使用毒苯酚、氯仿等试剂需要乙醇沉淀.
兔抗nf-κp65克隆抗体试剂
价格1元每盒
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