| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Norepinephrine ELISA kit - Urine/Plasma - Fast-Track |
| Description | In vitro diagnostics enzyme immunoassay (ELISA) for the rapid determination of Noradrenaline levels in plasma and urine samples. |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | 10 µl urine, 300 µl plasma |
| Cross specie-reactivity | React with all species |
| Standard range | 0 / 5 - 1000 ng/ml |
| Sensitivity | 1.5 ng/ml urine, 50 pg/ml plasma |
| Specificity | No significant cross-reactivity was observed with Noradrenaline analogs such as Dopamine, Adrenaline, Metanephrine, Normetanephrine, Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid. |
| Assay time | 4 hours |
| Storage | Store at 2-8°C for up to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample collection & storage | Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C. Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C. |
| Sample preparation | Sample preparation, extraction and acylation (65min) |
| ELISA | Norepinephrine antiserum incubation (2H), revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
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谢谢!
具体可以查看一下说明书。
谁用过亲水性改性的聚丙烯膜养过细胞,最好是whatman的,效果怎么样?十分感激!:)
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新帖要有题目,且要尽量展示帖子主要内容意思~bywzmc225
本版发帖须知及新手指南
1、在设备使用过程中,应对安全阀加以维修和检查,当设备闲置较长时间重新使用时应扳动安全阀上的小扳手,检查阀心是否灵活,防止弹簧因生锈影响安全阀跳起。同时设备工作时,当压力超过0.165Mpa时,安全阀不开启,应立即关闭电源,打开放气阀旋钮,当压力回到0时,稍等1-2min,在打开容器盖并及时更换安全阀。
2、压力表应定期检查,保证安全使用,若压力表指示不稳或不能恢复到0时,应及时检修或更换。
3、橡胶密封圈易老化变形,如有发现应及时更换。发现螺丝,螺母松动应及时加固。
4、堆放灭菌物品时,严禁堵塞安全阀的出气孔,必须留有空间保证其畅通放气。
每次使用前必须检查外桶内水量是否保持在灭菌桶搁脚处。
5、当灭菌持续时,在进行新的灭菌时,应留有5min的时间,并打开上盖让设备有时间冷却。
灭菌液体时,以不超过3/4体积为好,瓶口切勿使用未开孔的橡胶或软木塞。灭菌液体结束时不准立即释放蒸汽,必须待压力回到0时方可排放余汽。

