CLIA Kit for Hepatocyte Growth Factor (HGF)
HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen
- Product No.SCA047Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range2.74-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.14pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 588 For more details, please contact local distributors!US$ 840 For more details, please contact local distributors!US$ 3780 For more details, please contact local distributors!US$ 7140 For more details, please contact local distributors!US$ 58800 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Hepatocyte Growth Factor (HGF)
This assay has high sensitivity and excellent specificity for detection of Hepatocyte Growth Factor (HGF).No significant cross-reactivity or interference between Hepatocyte Growth Factor (HGF) and analogues was observed.
Recovery of the CLIA Kit for Hepatocyte Growth Factor (HGF)
Matrices listed below were spiked with certain level of recombinant Hepatocyte Growth Factor (HGF) and the recovery rates were calculated by comparing the measured value to the expected amount of Hepatocyte Growth Factor (HGF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 94-102 | 98 |
| EDTA plasma(n=5) | 84-102 | 89 |
| heparin plasma(n=5) | 94-103 | 98 |
Precision of the CLIA Kit for Hepatocyte Growth Factor (HGF)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the CLIA Kit for Hepatocyte Growth Factor (HGF)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hepatocyte Growth Factor (HGF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-98% | 81-96% | 93-101% | 92-101% |
| EDTA plasma(n=5) | 79-103% | 97-104% | 87-95% | 78-92% |
| heparin plasma(n=5) | 98-105% | 88-97% | 82-99% | 78-98% |
Stability of the CLIA Kit for Hepatocyte Growth Factor (HGF)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Hepatocyte Growth Factor (HGF)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;8. Read RLU value immediately.
Test principle of the CLIA Kit for Hepatocyte Growth Factor (HGF)
The microplate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Growth Factor (HGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepatocyte Growth Factor (HGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepatocyte Growth Factor (HGF) level in the sample or standard.;
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ELISA / CLIA Experiment Service
INCREMENT SERVICES
Single-component Reagents of Assay KitLysis Buffer Specific for ELISA / CLIAQuality Control of ELISA / CLIACLIA Kit Customized ServiceDisease Model Customized ServiceSerums Customized ServiceTGFB1 Activation ReagentReal Time PCR Experimental ServiceStreptavidin
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| APA047Hu02 | Active Hepatocyte Growth Factor (HGF) | Cell culture; Activity Assays. |
| RPA047Hu03 | Recombinant Hepatocyte Growth Factor (HGF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA047Hu01 | Active Hepatocyte Growth Factor (HGF) | Cell culture; Activity Assays. |
| RPA047Hu01 | Recombinant Hepatocyte Growth Factor (HGF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA047Hu02 | Recombinant Hepatocyte Growth Factor (HGF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA047Hu01 | Polyclonal Antibody to Hepatocyte Growth Factor (HGF) | WB; IHC; ICC; IP. |
| PAA047Hu02 | Polyclonal Antibody to Hepatocyte Growth Factor (HGF) | WB; IHC; ICC; IP. |
| PAA047Hu03 | Polyclonal Antibody to Hepatocyte Growth Factor (HGF) | WB; IHC; ICC; IP. |
| LAA047Hu71 | Biotin-Linked Polyclonal Antibody to Hepatocyte Growth Factor (HGF) | WB; IHC; ICC. |
| LAA047Hu53 | APC-Linked Polyclonal Antibody to Hepatocyte Growth Factor (HGF) | WB; IHC; ICC; IF. |
| LAA047Hu81 | FITC-Linked Polyclonal Antibody to Hepatocyte Growth Factor (HGF) | WB; IHC; ICC; IF. |
| MAA047Hu22 | Monoclonal Antibody to Hepatocyte Growth Factor (HGF) | WB; IHC; ICC; IP. |
| MAA047Hu23 | Monoclonal Antibody to Hepatocyte Growth Factor (HGF) | WB; IHC; ICC; IP. |
| SEA047Hu | ELISA Kit for Hepatocyte Growth Factor (HGF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA047Hu | Mini Samples ELISA Kit for Hepatocyte Growth Factor (HGF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA047Hu | CLIA Kit for Hepatocyte Growth Factor (HGF) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA047Hu01 | ELISA Kit DIY Materials for Hepatocyte Growth Factor (HGF) | Main materials for "Do It (ELISA Kit) Yourself". |
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酶标仪有单波长和双波长检测功能有时使用者不知在什么情况下使用单或双波长检测。所谓的“单波长”就是使用一种对显色具最大吸收的波长即450 nm或492 nm进行比色测定；而“双波长”则除了用对显色具最大吸收的波长即450 nm或492 nm进行比色测定外，同时用对特异显色不敏感的波长如630 nm进行测定，酶标仪最后打印出来的吸光度则为二者之差。630 nm波长下得到的吸光度是非特异的，来自于板子上诸如指纹、灰尘、脏物等所致的吸收。因此，在ELISA比色测定中，最好使用双波长，且不必设空白孔。
石英的96孔板，每个孔的体积为200ul，空白直接200ulDEPC水，实验组196ulPEPC水+4ulRNA
如果换了滤光片了，测试步骤与MTT测试步骤差不多，主要就是设置吸光度，点击四次吸光度，设置为340、280、260、230。
1、进板后确定板类型和板孔
2、设置吸光度，点击四次吸光度，分别设置为340、280、260、230。
3、开始
酶标仪即酶联免疫检测仪是酶联免疫吸附试验的专用仪器又称微孔板检测器。可简单地分为半自动和全自动2大类，但其工作原理基本上都是一致的，其核心都是一个比色计,即用比色法来进行分析。 测定一般要求测试液的最终体积在250μL以下，用一般光电比色计无法完成测试，因此对酶标仪中的光电比色计有特殊要求。
MPI是一种基于消息传递的并行编程技术，在不同节点计算机之间并行多进程执行程序（这种情况下，不同享内存），只是通过消息传递来进行通信，从而适用于分布式体系
第一种是，4，7，10三种校准后，再用7测试，看这次校准是否成功。
第二种是，4，校准完就立即用4测试，看看是否校准成功，7和10也一样，我想请问下，哪种方法是正确的，合理的，为什么？急！！！
帮帮忙，谢谢
