주문정보
- - 재고수량은 변동될 수 있습니다.
- - 재고 확인 시 "갱신" 버튼을 누르시면 실시간 재고를 확인하실 수 있습니다.
- - 가격이 ‘별도문의‘ 시, 상단 ‘견적신청’ 버튼을 눌러 문의해주시면 빠른 답변을 받으실 수 있습니다.
선택 | Cat.No. | 제품명 | 가격(VAT별도) | 수량 |
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제품특징
* 본 제품으로 합성한 cDNA는 Illumina와 Ion Torrent sequencing platform에 모두 적용 가능합니다.SMARTer Ultra Low Input RNAKit for Sequencing - v3는 매우 소량의 total RNA (10 pg-10 ng)나 single cell (1-1,000개의 cells)로부터 바로 RNA-Seq (Transcriptome analysis)에 이용할 수 있는cDNA를 제작할 수 있는 제품이다. 이전 버전의 SMARTer Ultra Low RNA Kit for Illumina Sequencing-HV 제품에 비하여 SMARTer Ultra Low Input RNA Kit for Sequencing - v3는 보다 많은 유전자를 동정할 수 있고, 높은 transcript mapping율을 보일 뿐만 아니라 더 높은 exon 커버율을보인다 (greater number of genes, higher transcript mapping, andhigher exon coverage). 또한 본 제품은 Illumina와 Ion Torrent 등 다양한 회사의 library preparationkit 또는 sequencing 기기에 적용 가능하다. 본 제품의 핵심 기술인 SMART (Switching Mechanism at 5’ End of RNA Template)법은 세포의splice junction과 alteranative splicing event를 포함한전반적인 전사체 연구 (full transcriptome analysis)에 있어 매우 유용한 기술이다. 또한, 본 제품은 high quality RNA (RIN > 8)나 intact cell로부터 특이적으로 mRNA를 증폭할 수 있다.
□ 특징
- Sample prep made easy - The single-tube protocol works directly on whole cells and preserves sample integrity
- Unparalleled sensitivity - Start with as little as 1 cell or 10 pg of total RNA (input range: 1-1,000 cells or 10 pg-10 ng of total RNA)
- Improved cDNA amplification - The SeqAmp DNA Polymerase included in the kit better amplifies high GC-content genes
- Suitable for multiple sequencing platforms - cDNA libraries are compatible with either Illumina® or Ion Torrent NGS platforms
- High-quality RNA-seq data - Get a higher number of genes identified, full length gene analysis, typically less than 2% rRNA reads, and improved representation of GC-rich genes
그림 1. Comparison of sequencing metricsgenerated with either the SMARTer Ultra Low Input RNA for Illumina Sequencingkit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). cDNA libraries were generatedfrom either 100 pg Human Brain Total RNA (HBR) or 10 pg Mouse Brain Total RNA(MBR) and were sequenced on an Illumina MiSeq platform. The increasedsensitivity gained using the UL-v3 protocol is most significant at the lowestRNA inputs.그림 2. Individualcells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) wereused as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3. The cDNA samples were analyzedon an Agilent 2100 Bioanalyzer. The single main peak indicates the purity andyield of the cDNA (the additional peak at ~1 kb in the HeLa cell samples is thecontribution of a single highly expressed gene, FTH1).그림 3. Sequencing results for librariesmade from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low InputRNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kitfor Sequencing - v3 (UL-v3). Genes were binned by GC content and correlationplots were used to evaluate the two kits. The average gene counts were veryreproducible for replicate samples using UL-HV (Panel A) or UL-v3 (Panel B).When the two protocols were compared, genes with a high GC content (red) showedhigher expression with the UL-v3 protocol while genes with a median or low GCcontent (gray and blue, respectively) showed similar expression levels withboth protocols (Panel C).그림 4. The gene body coverage of the cDNA libraries madefrom 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra LowInput RNA Kit for Sequencing - v3 was determined using RSeQC. Read coverage wasnormalized using Excel.그림 5. cDNA libraries were made from 1 or1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). Results of Illumina library preparation andsequencing. Table IIB. Results of Ion Torrent library preparation andsequencing.
□ 적용
● Transcriptome sequencing (RNA-Seq)을 위한 소량의 cell 또는 total RNA로부터 cDNA 합성
● 본 제품으로 합성한 cDNA는 Illumina sequencing platfomr에 적용하기 위하여, Clontech의 LowInput Library Prep Kit (code 634947) 또는 Nextra sample preparation kit을 이용할 수 있다. 또한 IonTorrent를 이용하기 위해서는 Ion Xpress Plus Fragment Library Kit을 사용 가능하다.
□ 구성품
* SMARTer Ultra Low Input RNAKit for Sequencing - v3 Components (Cat. No. 634854; not sold separately) * SeqAmp DNA Polymerase (Cat. No. 638504)
* SMARTer Ultra Low Input RNAKit for Sequencing - v3 (제품코드 634850) 종매 - 종매일 : 2017.11.27
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2、单光束分光光度计是由一束经过单色器的光,轮流通过参比溶液和样品溶液,以进行光强度测量。这种分光光度计的特点是:结构简单 价格便宜 主要适于做定量分析;
缺点是:测量结果受电源的波动影响较大,容易给定量结果带来较大误差,此外,这种仪器操作麻烦,不适于做定性分析
亲:希望我的回答能够帮助到你,如果满意,请采纳或点赞支持,给我更多助人的动力!
1,被测液浓度过高造成透过样品的光信号检测不到,这种情况下可以通过稀释样品来完成;
2,测定时,样品室内的样品架在移动时没移动到位造成挡光;
3,仪器内部的光源不亮或光源没有进入单色器中。可以对照以上情况检查分析!
可见光的波长范围是400nm~760nm,红620nm--760nm 橙592nm--620nm 黄578nm-- 592nm 绿500nm-- 578nm 青464nm--500nm 蓝446nm --464nm 紫400nm--446nm。
不能用玻璃比色皿是因为紫外线的玻璃的透射能力很低(玻璃吸收紫外光能力强),所以一般采用透射能力强的石英比色皿。
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希望和大家分享这一点小小的经验。
2、检测模式:荧光强度检测(FI), 荧光偏振检测(FP), 时间分辨荧光检测(TRF), TR-FRET, 高性能发光检测, 紫外/可见吸收光, AlphaScreen / AlphaLISA。
3、兼容 Take3™ 微量检测板 可进行体积为2 μL 的微量样品的检测
4、四光栅系统及带宽可调: Synergy H4 的光路整合了两个双光栅单色器. 这种四光栅设计. 可变的带宽设计极大的增加了检测的灵活性。
5、深度阻挡滤光片和二向色镜: Synergy H4的滤光片/二向色镜组合为,荧光强度、时间分辨荧光、荧光偏振及AlphaScreen / AlphaLISA 均具有最好的检测性能.向左转|向右转
请问,临床前研究用的电子天平,需要符合GMP要求吗?需要3Q认证吗?相关数据用于申报临床试验的,谢谢