Description:
UsetheCFX96DeepWellReal-TimePCRDetectionSystemforprecise,reproducIBLereal-timePCR.ThesystemiscomposedoftheCFX96DeepWellOpticalReactionModuleandtheC1000TouchThermalCyclerChassis.ThemoduleisamemberofthefamilyofinterchangeablereactionmodulesthatcanbeusedwiththeC1000TouchThermalCyclerChassisforPCRandreal-timePCR.Allmodulescanbequicklyinsertedandremovedfromthecyclerchassiswithouttools.
FeaturesandBenefits
- Deepwellsforlargevolumes
- Detectionofupto5targetsperwell,plusachanneldedicatedtosingleplexFRET
- Protocolautowriterthatgeneratesanoptimalprotocolforyourreactioncomponents
- ThermalgrADIentthatidentifiesoptimalannealingtemperatureinasinglerun
TheCFX96DeepWellReal-TimePCRDetectionSystemhassixindependentlycontrolledthermalelectricunitsprovidingeven,preciselycontrolledtemperaturesatalltimesduringtherun,includingramping.Thereducedmassofthehoneycombblockprovidesfastrampingandreducedsettlingtime(timetoachievethermaluniformity).
Theopticalsystem,withsixfilteredLEDsandsixfilteredphotodiodes,collectsdatafromallwells,detectinguptofivetargetsperwell.Forsingle-colorFAMandSYBR®GreenI,thefastscanoptionreadssingle-channelfluorescenceinall96wellsinjust3seconds.AchannelwithanLEDfilter–photodiodecombinationisdedicatedtoFRETsingleplexexperiments.
Thelargetouch-screeninterfacehasintuitivegraphicalprogramminganddisplaysdatatracesinrealtime.Theprogrammablethermalgradientfeaturecantesteighttemperaturesatoncetoquicklydeterminetheoptimalannealingtemperature,andtheprotocolautowriterquicklyoptimizesreactions,generatinganoptimalprotocolbasedonyourpolymerase,primers,andproductlength.
ProductComponents
- C1000TouchThermalCyclerChassis(1841100)
- CFX96DeepWellOpticalReactionModule(1840197)
- USBcable
- Instructionmanual
ConfigurationOptions
ThefollowingmodulescanbeusedwiththeC1000TouchThermalCyclerChassis:
| ReactionModules | CatalogNumber | |
| Module | ModulewithStarterPackage* | |
| ForPCR | ||
| Dual48/48FastReactionModule | 1840148 | — |
| 96-WellFastReactonModule | 1840196 | — |
| 96–DeepWellReactionModule | 1840197 | — |
| 384-WellReactionModule | 1840138 | — |
| ForReal-TimePCR | ||
| CFX96OpticalReactionModule | 1845097 | 1845096 |
| CFX96DeepWellOpticalReactionModule | 1844095 | 1844096 |
| CFX384™OpticalReactionModule | 1845385 | 1845384 |
*StarterpackagecontainsSsoAdvanced™UniversalSYBR®GreenSupermix(1725270),Microseal®"B"AdhesiveSeals(MSB1001),andHard-Shell®Low-ProfileThin-Wall96-WellSkirtedPCRPlates(HSP9601)orHard-ShellThin-Wall384-WellSkirtedPCRPlates(HSP3805).
TheCFX96DeepWellReal-TimePCRDetectionSystemcanbeconfiguredwithotherPCRinstruments,allowingstand-aloneorsoftwarecontrol.InstrumentpermutationsallowuptofourdifferentCFXConnect™,CFX96,orCFX384SystemstobecontrolledbyonePC.
CombineanytwoCFXReal-TimePCRDetectionSystemswiththeCFXAutomationSystemII,ourroboticplatehandler,tocreateahigh-throughput,automatedreal-timePCRsystemwithacompactfootprint.
ServicePlansandValidationToolsforCFXSystems
- Ensurethatyourreal-timePCRsystemsarefunctioningoptimallywithBio-RadExpertCareService;choosefromseveralserviceplanstokeepyourCFXAutomationSystemIIupandrunningitsbest
- CertifysysteminstallationandvalidatesystemperformancewithCFXSystemIQ/OQServicesandCFXAutomationSystemIIIQ/OQServices
- VerifytheperformanceofyourCFXSystemswithThermalValidationServicesandtheCFXQualificationPlates
RelatedProducts
- PX1™PCRPlateSealer
- PCRplasticsoptimizedforCFXReal-TimePCRDetectionSystems:
- Hard-Shell96-WellLowProfileSkirtedPCRPlatesincludingbarcodedplates(HSP9955)
- Microseal"B"AdhesiveSeals(MSB1001)
- OpticallyClearHeatSeals(1814030)
- CFXMaestroSoftware,SecurityEdition,forcompliancewithU.S.FDA21CFRPart11regulations
- CFXQualificationPlateforperformanceverification,96-well (1845098)
RelatedCategories
- Real-TimePCRDetectionSystems
- PCRReagentsandqPCRReagents
- PCRPlasticConsumables
- PCRSeals
- PCRAnalysisSoftware
SupportingDocuments
- CFX96Touch™DeepWellReal-TimePCRDetectionSystemSpecificationsSheet
- CFX96TouchandCFX96TouchDeepWellReal-TimePCRDetectionSystemsInstallationQuickGuide
- CFX96Touch,CFX96TouchDeepWell,andCFX384TouchReal-TimePCRDetectionSystemsInstructionManual
- OpticalDesignofCFX96Real-TimePCRDetectionSystemEliminatestheRequirementofaPassiveReferenceDyeApplicationNote
- Real-TimePCRApplicationsGuide
OnlineResources
- Real-TimePCRDetectionSystemsSelectionChart
- qPCR/Real-TimePCR
- qPCRInstrumentation
Specifications:
| ThermalCycler | |
| Chassis | C1000Touch™ |
| Maximumramprate,°C/sec | 2.5 |
| Averageramprate,°C/sec | 2 |
| Heatingandcoolingmethod | Peltier |
| Lid,°C | Heatsupto105 |
| Temperature | |
| Range,°C | 0–100 |
| Accuracy,°C | ±0.2ofprogrammedtargetat90°C |
| Uniformity,°C | ±0.4well-to-wellwithin10secofarrivalat90°C |
| Gradient | |
| Operationalrange,°C | 30–100 |
| Programmablespan,°C | 1–24 |
| OpticalDetection | |
| Excitation | 6filteredLEDs |
| Detection | 6filteredphotodiodes |
| Rangeofexcitation/emissionwavelengths,nm | 450–730 |
| Sensitivity | Detects1copyoftargetsequenceinhumangenomicDNA |
| Dynamicrange | 10ordersofmagnitude |
| ScanTime | |
| Allchannels,sec | 12 |
| FAM/SYBR®Greenonly,sec | 3 |
| Software | |
| Operatingsystems | WindowsXP,Windows7 |
| Multiplexanalysis | Upto5targetsperwell |
| System | |
| Licensedforreal-timePCR | Yes |
| Samplecapacity,wells | 96 |
| Samplesize,µl | 10–125 |
| Communicationinterface | USB2.0 |
| Electricalapprovals | IEC,CE |
| Dimensions(WxDxH),cm/in | 33x46x36/13x18x14 |
| Weight,kg/lb | 21/47 |
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做完实验之后放8联排的卡槽怎么按都弹不出来了。求助各位可能的原因有哪些？原因越详细越多越好，避免下次再出现这种状况，有没有其他战友遇到过这种情况？谢谢！
各位大神，有使用过ThermoPX2这个型号的pcr扩增仪吗？
PCR技术类似于DNA的天然复制过程，其特异性依赖于与靶序列两端互补的寡核苷酸引物,由变性--退火--延伸三个基本反应步骤构成
普通PCR的孔所有温度是一样的。
请问PCR仪设置加样量有什么作用，我们实验室有的pcr仪可以设置有的不能设置。如果我用了25微升体系，pcr仪设置时设置了50微升体系会怎么样呢？帮忙回答一下。谢谢~
