Highlights
- Removes PCR inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanin, etc. from nucleic acid solutions to yield high quality DNA or RNA.
- Fast, one-step procedure for cleaning impure samples prior to PCR, sequencing, reverse transcription (RT), etc.
Description
Applicable For | Cleaned samples are ready for PCR, sequencing, reverse transcription (RT), etc. |
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Elution Volume | 50 - 200 µl |
Equipment | Microcentrifuge |
Purity | High quality, enzymatic-reaction ready DNA/RNA products. |
Sample Source | DNA/RNA samples contaminated with enzymatic reaction inhibitors. |
Sample Storage | Eluted DNA/RNA can be used immediately or stored at ≤ -20°C. |
Type | DNA and RNA |
Yield | 50 - 90% recovery |
Q1: What is the smallest input volume of DNA that can be added to the OneStep Inhibitor Removal kit?
The smallest volume will be 50 ul.
Q2: What is the largest input volume of DNA that can be added to the OneStep Inhibitor Removal kit?
The OneStep PCR Inhibitor Removal Kit can process about 500 µl of sample at a time. However, the same column can be reused for the same sample.
Q3: Can I centrifuge at a lower speed than 3,500 x g for the 96-well kit?
No, the kit requires the speed to be at 3,500 x g. Centrifuging at lower speeds can result in inefficient sample elution.
“The ease.Holy cow, this thing was so freaking simple compared to similar products”
-Christian H. (University of Georgia)
“It was VERY fast and yielded the intended results...PCR products from previously non-amplifiable DNA.”
-Tara N. (United States Agricultural Research Service)
Cat # | Name | Size | Price | |
---|---|---|---|---|
C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
D6035-1-30 | Prep Solution | 30 ml | $18.00 | |
C1058-50 | Zymo-Spin III-HRC Filters | 50 Pack | $108.00 | |
C2003 | Elution Plate | 2 Plates | $19.00 | |
C2009 | Silicon-A-HRC Plate | 2 Plates | $405.00 |
ebiomall.com
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谢谢!
2、仪器功能:进口和国产仪器主要功能:如保存,过温保护,断电保护,热盖功能等基本上是一样的,只是附带功能上有所差别,比如:进口的显示屏是大型的触摸式荧屏操作,国产的大多是触摸键,荧屏显示操作,进口仪器有的可以升级为荧光定量PCR仪,而国产的目前主要是专用机。而这些仪器附带功能的差别大多都是最近两年新推出来的,价格确是相差几倍。有的进口仪器没有这些附带的特殊功能,从性能和功能上比有的等于或低于国产的。
各位大神,有使用过ThermoPX2这个型号的pcr扩增仪吗?
普通PCR的孔所有温度是一样的。