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Specifications
Print Version
| Description | SafeView |
|---|---|
| SKU | G468 |
| SafeView Series | Post Staining Dye |
| LED Viewer Compatibility | Yes |
| Stain Color | Green |
| Applications | Safe Detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels. |
| Note | All SafeView DNA Stains are ISO-13485 certified. Dispose of SafeView DNA Stains as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide). |
| Shipping Conditions | Shipped on blue ice packs. |
| Storage Condition | Store at 4°C for up to 2 years. |
| Unit quantity | 1.0 ml (10,000X) |
Documents
- SafeView Plus Protocol
- SafeView Plus™
- Sensitivity Test
- SafeView Plus Promotional Flyer
FAQs
| Can SafeView be a replacement for ethidium bromide? Can I do gel extraction with it? | |
SafeView can be used as a replacement for ethidium bromide as they both work on general agarose. We recommend using SafeGreen for downstream cloning applications as SafeView can interfere with the ligation reaction, yielding fewer colonies. | |
| How does SafeView work and why is it not carcinogenic? | |
There are fluorescent compounds in SafeView and these fluorescent compounds have the capability to bind to DNA. There may be some unknown effects of SafeView that have not been documented but that applies to the SYBR set as well; however, SafeView products are definitely not as carcinogenic as ethidium bromide. | |
| How do I use SafeView products? | |
The Safe-(Red, Green and White) loading dyes work the same as 6x loading dye, loaded with the sample. SafeView Classic is used directly in the gel and the running buffer. | |
| Does the SafeView differentiate double stranded nucleic acid and single stranded nucleic acid? Does the Safe-(Red, Green and White) work the same way? | |
Under UV, SafeView Classic emits a green fluorescence when bound to both single and double stranded DNA templates, therefore they cannot be differentiated by this method. It will emit a red fluorescence when bound to RNA templates.The SafeView Stains (Red, Green and White) do not perform in this way and will stain all nucleic acid templates one color. | |
| At what temperature do I store the SafeView products? | |
All the SafeView products should be stored at four degrees Celsius. | |
| Do I need a special filter for photography of DNA gels stained with SafeView? | |
Under UV light, SafeView Classic emits a green fluroescence when bound to both single and double stranded DNA templates. It will emit a red fluorescence when bound to RNA templates. No filter is necessary for viewing these colours, however a filter may be needed for photographing the gel. | |
| How long does the SafeView Classic stain last in a gel? | |
Our in-house testing has shown that SafeView stained gels (>10ng DNA loaded per lane) can still be effectively visualized up to 1 week later with only a slight decrease in brightness. Gels should be stored properly to maintain a good signal, at 4C in the dark, sealed in a plastic bag or pouch with wet paper towel loosely wrapped around the gel. | |
| Why is SafeView (G-108) stain not working on my samples? | |
Make sure you are following the protocol carefully. It is critical that both buffer and gel have SafeView dye in them otherwise it will not work. This is different from ethidium bromide. You can consider to add 2.5ul of SafeView (instead of 5ul) for every 100ml of running buffer, which will reduce background fluorescence and allow the bands to show with more contrast. | |
| Is it degradable, if so how fast and under what circumstances? | |
2 hours over 100C | |
| What is the sensitivity of the dyes? | |
Safe-Green has Excitation Wavelength of 490nm and Emission Wavelength of 525nm, and its sensitivity range is between 0.2-0.6ng.Safe-Red has Excitation Wavelength of 540nm and Emission Wavelength of 630nm, and its sensitivity range is between 0.3-0.8ng.Safe-White has Excitation Wavelength of 370nm and Emission Wavelength of 470nm, and its sensitivity range is between 0.2-0.5ng.SafeView Classic emits green fluorescence when bound to dsDNA and ssDNA and red fluorescence when bound to RNA. This stain has one excitation (490 nm) and two emission spectra (520 nm and 635 nm) and the sensitivity range is between 0.1-0.3ng.SafeView Plus has Excitation Wavelength of 490nm and Emission Wavelength of 525nm and its sensitivity range is between 0.05-0.1ng. | |
| Can SafeView products be used post-stain? | |
Only SafeView Plus (G468) should be used in a post stain. SafeView classic and Safe Stains are not designed for post-staining. SafeView Classic must be added to the gel and the running buffer prior to the loading of the samples. Safe-(Red, Green and White) stains must be added to the sample before loading it to the gel. | |
| Why is the EtBr signal stronger in the pictures when I compare SafeView with EtBr? | |
A reason for this is that most gel doc systems have been optimized for EtBr so that is why the EtBr signal may be stronger in the pictures. | |
| will I need an additional loading buffer for my samples? | |
The loading dye is included in the products. No additional loading buffer needed. | |
| We see migrations and band shifting of our fragments. Are there any recommendations that you can give us to minimize this band shifting? | |
Shifting is unavoidable and quite natural for any fragments, regardless of the staining agent. We suggest to use SafeGreen ladders, which will give accurate molecular weight with no additional staining agents needed.http://www.abmgood.com/DNA-Ladder-Safe-Green™-100bp-Opti-DNA-Marker-Invitroge-G473.htmlhttp://www.abmgood.com/DNA-Ladder-Safe-Green™-1kb-Opti-DNA-Marker-Invitroge-G474.html | |
| We see shifting and migration of the DNA fragments. What are the recommendations to minimize this? | |
Shifting is unavoidable and quite natural for such fragments, regardless of the staining. We suggest using SafeGreen ladders, which will give accurate molecular weight with no additional staining agents needed.http://www.abmgood.com/DNA-Ladder-Safe-Green™-100bp-Opti-DNA-Marker-Invitroge-G473.htmlhttp://www.abmgood.com/DNA-Ladder-Safe-Green™-1kb-Opti-DNA-Marker-Invitroge-G474.html | |
| I cannot see 100bp and 200bp bands on a 1% gel. What should I do? | |
It is very difficult to detect 100bp and 200bp bands in 1% gel with any stains. Higher gel concentrations should be used, such as 2% agarose. | |
| Can I use SafeView products in Polyacrylamide gels? | |
Yes, we have tested our SafeView products for this application. | |
| Which of the Safe stains will work with blue light / LED? | |
All of our SafeView stains have been tested in-house to be compatible with UV light. SafeView Classic, SafeView Plus, and SafeGreen will also work under blue light/LED. SafeRed and SafeWhite will only work under UV light.Safe View Plus should only appear green. SafeView Classic will be red for RNA and green for DNA. | |
| What is the approved filter for eliminating the spent running buffer solutions? | |
Most facilities have approved the disposal of SafeView chemicals directly down the drain, with adequate water flushing or dilution, since SafeView™ products are non-carcinogenic. However, regulations vary between different regions, so please contact your local safety office for disposal guidelines specific to your area. You may review the MSDS of this product for more detailed information. | |
References
- Dlusskaya, E. A., Atrazhev, A. M., & Ashbolt, N. J. "Colloid chemistry pitfall for flow cytometric enumeration of viruses in water" Water Research X 2:100025 (2019). DOI: 10.1016/j.wroa.2019.100025.
- Pereira, B. A., Zangeronimo, M. G., Castillo-Martín, M., Gadani, B., Chaves, B. R., Rodríguez-Gil, J. E., … Yeste, M. "Supplementing Maturation Medium With Insulin Growth Factor I and Vitrification-Warming Solutions With Reduced Glutathione Enhances Survival Rates and Development Ability of in vitro Matured Vitrified-Warmed Pig Oocytes" Frontiers in Physiology 9: (2019). DOI: 10.3389/fphys.2018.01894.
蚂蚁淘电商平台
ebiomall.com
ebiomall.com
公司简介
蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，
该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁
淘搜索全球供应信息，找到合适的产品后在蚂蚁淘下单，然后蚂蚁淘的海外买手进行跨境采购、
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蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时， 您可通过我的订单提交您的“申请售后”， 蚂蚁淘产品顾问会第一时间为您处理， 在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线：4000-520-616。
2021-07-14
从RNA提取到基因的定量研究，已经成为一条经典的研究策略；其在科研、临检、进出口、公安等众多领域的应用日益广泛。不过由于RNA提取以及荧光定量PCR实验的有效性和可信度要求，使得这一实验方案在实验操作、实验设计以及实验数据分析方面，对于实验人员的要求越来越高。另外，荧光定量仪器价格高昂，实验室的普及程度远低于常规PCR仪，这也成为科研工作者掌握此 查看更多
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我院将于近日PCR仪的招标，欢迎合格投标人前来咨询投标。 医院评标小组将本着公开、公平、合理的原则给各投标商提供公平竞争的机会，不得提供虚假材料或者诽谤排挤其他供应商。严禁采用不正当方法了解采购信息或挤压当事人，与采购人员以及供应商之间不负责任的串通消息。在整个招标过程中不允许对院内任何部门任何人许愿条件。 投标商对招标采购事项及评标结果有疑问者应首先向设备部门有关人员询问和沟通，答复不满意时，请向医院监审部门反映。请贵公司积极 查看更多
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2018-01-10
苏州蚂蚁淘生物科技有限公司推出“Bio-Rad伯乐T100PCR仪全国联保|维修服务”服务热线:4000-520-616（限工作日9:00-18:00）固话：0512-67156496手机：18915418616QQ：3507623898、3464842061 伯乐PT100PCR仪。PT100是伯乐公司推出的一款经济型的PCR仪。特别适合于简单场合的应用，学校是这个机器的主要用户。此机的故障是，运行程序的过程中，不定时的报警。检查机器的电源，主控制板均没有问题 查看更多
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bio-rad伯乐 C1000 Touch PCR仪 bio-rad pcr仪 货号：185-1196，185-1197，185-1138，185-1148C1000 Touch PCR仪快速、可靠、具有精湛的温控性能。模块化设计可实现反应模块的快速更换，且无需额外工具，能满足不同实验的要求。可升级荧光定量PCR检测模块。bio-rad伯乐 C1000 Touch PCR仪 bio-rad pcr仪bio-rad梯度生成仪bio-rad 查看更多
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bio-rad伯乐 T100 PCR仪 bio-rad pcr仪货号：1861096T100 PCR仪传承Bio-Rad性能稳定、技术创新的理念，是科研人员的明智之选。流线型简洁设计的T100 PCR仪包括诸多优点，让您的PCR实验如此简单。bio-rad伯乐 T100 PCR仪 bio-rad pcr仪bio-rad梯度生成仪bio-rad核酸蛋白测定仪bio-rad细胞计数仪bio-rad转基因系统bio-rad脉冲场电泳槽bio- 查看更多
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产地 Applied BiosystemsABI Stepone Plus实时荧光定量PCR仪现货 厂商性质 经销商...美国应用生物系统公司推出的采用创新性技术的实时PCR扩增仪StepOnePlus™,操作... 查看更多
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梯度PCR仪是由普通PCR仪衍生出的带梯度PCR功能的基因扩增仪。PCR反应能否成功，退火温度是关键，梯度PCR仪每个孔的温度可以在指定范围内按照梯度设置。... 查看更多
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2018-02-26
江西宏广招标咨询有限公司受景德镇市畜牧兽医站委托，对其所需的货物和相关服务进行国内询价采购欢迎符合资格条件的供应商投标。1、招标编号：JXHG2018-JDZ-003号2、项目招标内容：序号项目编号货物名称数量单位预算总价（万元）规格型号及技术参数要求1景购2018B000027640荧光定量PCR仪仪器设备采购项目1台￥23详见询价文件注：本项目采购国内产品，不允许提供进口产品参与采购活动。3、投标人的资格条件：（1）投标人应具有独立 查看更多
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ABI 2070 伯乐 T100 现货 查看更多
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Ebiomall是跨境直采平台，我们直接从厂家采购，自己的团队负责国际物流和清关，中间没有第三方，蚂蚁淘承诺所售产品仅为正品，假一罚十。
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商品咨询
PCR仪百科简介爱仪器仪表网123
遗忘VPBS2017-10-25
简单的说，PCR就是利用DNA聚合酶对特定基因做体外或试管内In Vitro的大量合成，基本上它是利用DNA聚合酶进行专一性的连锁复制。目前常用的技术，可以将一段基因复制为原来的一百亿至一千亿倍。根据DNA扩增的目的和检测的标准，可以将PCR仪分为普通PCR仪，梯度PCR仪，原位PCR仪，实时荧光定量PCR仪四类。向左转|向右转
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请问PCR仪设置加样量有什么作用 分子生物 PCR相关 ...123
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请问PCR仪设置加样量有什么作用，我们实验室有的pcr仪可以设置有的不能设置。如果我用了25微升体系，pcr仪设置时设置了50微升体系会怎么样呢？帮忙回答一下。谢谢~
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梯度PCR可以让不同的孔有不同温度，比如横向有12个孔。退火温度可以从低到高。
普通PCR的孔所有温度是一样的。
普通PCR的孔所有温度是一样的。
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推荐去赛飞生物科技了解咨询。。。
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