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Specifications
Print Version
| Description | SafeView |
|---|---|
| SKU | G468 |
| SafeView Series | Post Staining Dye |
| LED Viewer Compatibility | Yes |
| Stain Color | Green |
| Applications | Safe Detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels. |
| Note | All SafeView DNA Stains are ISO-13485 certified. Dispose of SafeView DNA Stains as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide). |
| Shipping Conditions | Shipped on blue ice packs. |
| Storage Condition | Store at 4°C for up to 2 years. |
| Unit quantity | 1.0 ml (10,000X) |
Documents
- SafeView Plus Protocol
- SafeView Plus™
- Sensitivity Test
- SafeView Plus Promotional Flyer
FAQs
| Can SafeView be a replacement for ethidium bromide? Can I do gel extraction with it? | |
SafeView can be used as a replacement for ethidium bromide as they both work on general agarose. We recommend using SafeGreen for downstream cloning applications as SafeView can interfere with the ligation reaction, yielding fewer colonies. | |
| How does SafeView work and why is it not carcinogenic? | |
There are fluorescent compounds in SafeView and these fluorescent compounds have the capability to bind to DNA. There may be some unknown effects of SafeView that have not been documented but that applies to the SYBR set as well; however, SafeView products are definitely not as carcinogenic as ethidium bromide. | |
| How do I use SafeView products? | |
The Safe-(Red, Green and White) loading dyes work the same as 6x loading dye, loaded with the sample. SafeView Classic is used directly in the gel and the running buffer. | |
| Does the SafeView differentiate double stranded nucleic acid and single stranded nucleic acid? Does the Safe-(Red, Green and White) work the same way? | |
Under UV, SafeView Classic emits a green fluorescence when bound to both single and double stranded DNA templates, therefore they cannot be differentiated by this method. It will emit a red fluorescence when bound to RNA templates.The SafeView Stains (Red, Green and White) do not perform in this way and will stain all nucleic acid templates one color. | |
| At what temperature do I store the SafeView products? | |
All the SafeView products should be stored at four degrees Celsius. | |
| Do I need a special filter for photography of DNA gels stained with SafeView? | |
Under UV light, SafeView Classic emits a green fluroescence when bound to both single and double stranded DNA templates. It will emit a red fluorescence when bound to RNA templates. No filter is necessary for viewing these colours, however a filter may be needed for photographing the gel. | |
| How long does the SafeView Classic stain last in a gel? | |
Our in-house testing has shown that SafeView stained gels (>10ng DNA loaded per lane) can still be effectively visualized up to 1 week later with only a slight decrease in brightness. Gels should be stored properly to maintain a good signal, at 4C in the dark, sealed in a plastic bag or pouch with wet paper towel loosely wrapped around the gel. | |
| Why is SafeView (G-108) stain not working on my samples? | |
Make sure you are following the protocol carefully. It is critical that both buffer and gel have SafeView dye in them otherwise it will not work. This is different from ethidium bromide. You can consider to add 2.5ul of SafeView (instead of 5ul) for every 100ml of running buffer, which will reduce background fluorescence and allow the bands to show with more contrast. | |
| Is it degradable, if so how fast and under what circumstances? | |
2 hours over 100C | |
| What is the sensitivity of the dyes? | |
Safe-Green has Excitation Wavelength of 490nm and Emission Wavelength of 525nm, and its sensitivity range is between 0.2-0.6ng.Safe-Red has Excitation Wavelength of 540nm and Emission Wavelength of 630nm, and its sensitivity range is between 0.3-0.8ng.Safe-White has Excitation Wavelength of 370nm and Emission Wavelength of 470nm, and its sensitivity range is between 0.2-0.5ng.SafeView Classic emits green fluorescence when bound to dsDNA and ssDNA and red fluorescence when bound to RNA. This stain has one excitation (490 nm) and two emission spectra (520 nm and 635 nm) and the sensitivity range is between 0.1-0.3ng.SafeView Plus has Excitation Wavelength of 490nm and Emission Wavelength of 525nm and its sensitivity range is between 0.05-0.1ng. | |
| Can SafeView products be used post-stain? | |
Only SafeView Plus (G468) should be used in a post stain. SafeView classic and Safe Stains are not designed for post-staining. SafeView Classic must be added to the gel and the running buffer prior to the loading of the samples. Safe-(Red, Green and White) stains must be added to the sample before loading it to the gel. | |
| Why is the EtBr signal stronger in the pictures when I compare SafeView with EtBr? | |
A reason for this is that most gel doc systems have been optimized for EtBr so that is why the EtBr signal may be stronger in the pictures. | |
| will I need an additional loading buffer for my samples? | |
The loading dye is included in the products. No additional loading buffer needed. | |
| We see migrations and band shifting of our fragments. Are there any recommendations that you can give us to minimize this band shifting? | |
Shifting is unavoidable and quite natural for any fragments, regardless of the staining agent. We suggest to use SafeGreen ladders, which will give accurate molecular weight with no additional staining agents needed.http://www.abmgood.com/DNA-Ladder-Safe-Green™-100bp-Opti-DNA-Marker-Invitroge-G473.htmlhttp://www.abmgood.com/DNA-Ladder-Safe-Green™-1kb-Opti-DNA-Marker-Invitroge-G474.html | |
| We see shifting and migration of the DNA fragments. What are the recommendations to minimize this? | |
Shifting is unavoidable and quite natural for such fragments, regardless of the staining. We suggest using SafeGreen ladders, which will give accurate molecular weight with no additional staining agents needed.http://www.abmgood.com/DNA-Ladder-Safe-Green™-100bp-Opti-DNA-Marker-Invitroge-G473.htmlhttp://www.abmgood.com/DNA-Ladder-Safe-Green™-1kb-Opti-DNA-Marker-Invitroge-G474.html | |
| I cannot see 100bp and 200bp bands on a 1% gel. What should I do? | |
It is very difficult to detect 100bp and 200bp bands in 1% gel with any stains. Higher gel concentrations should be used, such as 2% agarose. | |
| Can I use SafeView products in Polyacrylamide gels? | |
Yes, we have tested our SafeView products for this application. | |
| Which of the Safe stains will work with blue light / LED? | |
All of our SafeView stains have been tested in-house to be compatible with UV light. SafeView Classic, SafeView Plus, and SafeGreen will also work under blue light/LED. SafeRed and SafeWhite will only work under UV light.Safe View Plus should only appear green. SafeView Classic will be red for RNA and green for DNA. | |
| What is the approved filter for eliminating the spent running buffer solutions? | |
Most facilities have approved the disposal of SafeView chemicals directly down the drain, with adequate water flushing or dilution, since SafeView™ products are non-carcinogenic. However, regulations vary between different regions, so please contact your local safety office for disposal guidelines specific to your area. You may review the MSDS of this product for more detailed information. | |
References
- Dlusskaya, E. A., Atrazhev, A. M., & Ashbolt, N. J. "Colloid chemistry pitfall for flow cytometric enumeration of viruses in water" Water Research X 2:100025 (2019). DOI: 10.1016/j.wroa.2019.100025.
- Pereira, B. A., Zangeronimo, M. G., Castillo-Martín, M., Gadani, B., Chaves, B. R., Rodríguez-Gil, J. E., … Yeste, M. "Supplementing Maturation Medium With Insulin Growth Factor I and Vitrification-Warming Solutions With Reduced Glutathione Enhances Survival Rates and Development Ability of in vitro Matured Vitrified-Warmed Pig Oocytes" Frontiers in Physiology 9: (2019). DOI: 10.3389/fphys.2018.01894.
蚂蚁淘电商平台
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ebiomall.com
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蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，
该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁
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商品咨询
PCR_PCR仪器论坛社区123
一壶泊酒2018-01-22
【求助/交流】请问大家都是用的哪种PCR仪 生物科学 ...123
jwzh82008-06-07
最近要做DGGE,想买一台PCR仪。我主要做土壤微生物生态，想用PCR仪P16SrDNA，请大家根据自己的经验帮忙推荐一下，最好是主流品牌，性价比高一点的，国产进口都行；也请推荐一下凝胶成像系统。
谢谢！
谢谢！
进口PCR仪与国产PCR仪的区别在于哪里高压灭菌锅|高压灭菌器|进口...123
花开AA02352017-10-27
1、仪器性能：进口PCR以，和国产的PCR仪，在技术和使用性能上基本上没有多大的差别，比如：升降温速率，孔间温差，温度均一性，梯度温度范围，热盖温度等性能指标，国内外仪器基本达到了相同水平。而其差别主要是在功能上的差别，价格确实相差几倍。
2、仪器功能：进口和国产仪器主要功能：如保存，过温保护，断电保护，热盖功能等基本上是一样的，只是附带功能上有所差别，比如：进口的显示屏是大型的触摸式荧屏操作，国产的大多是触摸键，荧屏显示操作，进口仪器有的可以升级为荧光定量PCR仪，而国产的目前主要是专用机。而这些仪器附带功能的差别大多都是最近两年新推出来的，价格确是相差几倍。有的进口仪器没有这些附带的特殊功能，从性能和功能上比有的等于或低于国产的。
2、仪器功能：进口和国产仪器主要功能：如保存，过温保护，断电保护，热盖功能等基本上是一样的，只是附带功能上有所差别，比如：进口的显示屏是大型的触摸式荧屏操作，国产的大多是触摸键，荧屏显示操作，进口仪器有的可以升级为荧光定量PCR仪，而国产的目前主要是专用机。而这些仪器附带功能的差别大多都是最近两年新推出来的，价格确是相差几倍。有的进口仪器没有这些附带的特殊功能，从性能和功能上比有的等于或低于国产的。
PCR仪如何选型?123
迷迭逆夏000692017-10-25
定量PCR仪主要由两部分组成，一个是PCR系统，一个是荧光检测系统。选择定量PCR仪的关键——由于定量PCR必需借助样本和标准品之间的对比来实现定量的，对于定量PCR系统来说，重要的参数除了传统PCR的温控精确性、升降温速度等等，更重要的还在于样品孔之间的均一性，以避免微小的差别被指数级放大。至于荧光检测系统，多色多通道检测是当今的主流趋势——仪器的激发通道越多，仪器适用的荧光素种类越多，仪器适用范围就越宽；多通道指可同时检测一个样品中的多种荧光，仪器就可以同时检测单管内多模版或者内标 样品，通道越多，仪器适用范围越宽、性能就更强大。
大家都用什么牌子的PCR仪器? 123
莫邪9椱2021-07-23
国内实验室用的最多的有Thermo的和Biorad的，属于国外品牌，有很多个型号可供选择。国产最多的是博日的，还有一些其他牌子的。
real time PCR的试剂盒可以在普通的PCR仪做普通的PCR吗 123
leomed2021-07-28
由于自己实验室没有realtime-PCR的仪器，所以要到别的实验室做，但是每次操作的费用比较高，而自己的实验室有普通PCR的仪器，所以我想问下我能否在普通的PCR上用realtime-PCR的试剂（SYBRgreen）和引物先摸索合适的反应条件，然后再应用到realtime-PCR的仪器中？如果可以需要注意哪些方面呢?非常感谢！:)
【求助】第一次用ABI 7500做PCR得到的EDS文件怎么打开？ 核酸基...123
xinlv2018-01-22
PCR报价_PCR_基因扩增仪品牌_厂家123
guojing郭晶2021-07-29
各位大神，有使用过ThermoPX2这个型号的pcr扩增仪吗？
BioRad伯乐CFX96 Touch 实时荧光定量PCR仪_技术特点123
yilir2021-08-06
梯度PCR仪与普通PCR仪的区别是什么? 分析行业新闻123
然然﹋jhgo2017-10-25
梯度PCR可以让不同的孔有不同温度，比如横向有12个孔。退火温度可以从低到高。
普通PCR的孔所有温度是一样的。
普通PCR的孔所有温度是一样的。
博日Life ECO基因扩增仪 国产PCR仪_pcr仪,国产pcr仪,博日pcr...123
sen20292017-10-25
国产的最好的当属博日PCR了，他们是大和热磁的子公司，他们pcr的核心部件半导体就是大和热磁生产的，比一些进口pcr用的材质都要好很多的，我们是博日的签约代理商。 当然了，不是我们代理他们东西就说他们的好，另外还有朗基，依科赛等也不错。
美国鲍希尔公司北京代表处 POSSEHL INC. GROUP ...123
世纪鸟2008-07-15
本实验室计划购买PCR仪器一台,请大家帮忙推荐一下.最好带梯度的.BIORAD有一种,说是可以升级为定量的,不知道效果怎样,型号是icycler170-8720,请大家多指点.
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