PrimeDirect Probe RT-qPCR Mix is designed for one-step, real-time RT-PCR via probe detection (5′-nuclease method). This product is supplied as a 2X premix to facilitate the easy preparation of reaction mixtures. It requires only the addition of your primer, probe, and sample, and the RT-qPCR can be performed by simply adding your sample directly to the reaction mixture without intervening nucleic acid purification steps.
PrimeDirect Probe RT-qPCR Mix is designed for one-step, real-time RT-PCR via probe detection (5′-nuclease method). This product is supplied as a 2X premix to facilitate the easy preparation of reaction mixtures. It requires only the addition of your primer, probe, and sample, and the RT-qPCR can be performed by simply adding your sample directly to the reaction mixture without intervening nucleic acid purification steps.
The protocol allows all steps, from extraction of the nucleic acid to reverse transcription and qPCR, to be performed in a single tube, greatly reducing hands-on time and risk of contamination. The single-tube workflow also allows the detection of RNA viruses with complex higher-order structures.
High-efficiency PCR after cDNA synthesis detects DNA amplification in real time by measuring increased fluorescence from the probe. Because this product is highly resistant to various PCR inhibitors such as heparin (blood) and humic acid (soil), it can be used to directly detect pathogens, including viruses and bacteria, in various biological specimens and to directly analyze gene expression in cells.
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请问PCR仪设置加样量有什么作用，我们实验室有的pcr仪可以设置有的不能设置。如果我用了25微升体系，pcr仪设置时设置了50微升体系会怎么样呢？帮忙回答一下。谢谢~
普通PCR的孔所有温度是一样的。
