PrimeDirect Probe RT-qPCR Mix is designed for one-step, real-time RT-PCR via probe detection (5′-nuclease method). This product is supplied as a 2X premix to facilitate the easy preparation of reaction mixtures. It requires only the addition of your primer, probe, and sample, and the RT-qPCR can be performed by simply adding your sample directly to the reaction mixture without intervening nucleic acid purification steps.
PrimeDirect Probe RT-qPCR Mix is designed for one-step, real-time RT-PCR via probe detection (5′-nuclease method). This product is supplied as a 2X premix to facilitate the easy preparation of reaction mixtures. It requires only the addition of your primer, probe, and sample, and the RT-qPCR can be performed by simply adding your sample directly to the reaction mixture without intervening nucleic acid purification steps.
The protocol allows all steps, from extraction of the nucleic acid to reverse transcription and qPCR, to be performed in a single tube, greatly reducing hands-on time and risk of contamination. The single-tube workflow also allows the detection of RNA viruses with complex higher-order structures.
High-efficiency PCR after cDNA synthesis detects DNA amplification in real time by measuring increased fluorescence from the probe. Because this product is highly resistant to various PCR inhibitors such as heparin (blood) and humic acid (soil), it can be used to directly detect pathogens, including viruses and bacteria, in various biological specimens and to directly analyze gene expression in cells.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
普通PCR的孔所有温度是一样的。
谢谢！
PCR技术类似于DNA的天然复制过程，其特异性依赖于与靶序列两端互补的寡核苷酸引物,由变性--退火--延伸三个基本反应步骤构成
各位大神，有使用过ThermoPX2这个型号的pcr扩增仪吗？
