The Bcl-2 gene family encodes a set of proteins involved in apoptotic inhibition, which are commonly analyzed during the performance of a Bcl-2 apoptosis assay. The ApoPrimer Set (Bcl-2 family) for RT-PCRis composed of a set of primers used toamplify cDNA derived from the mRNA of seven Bcl-2 family members (mcl-1, bfl-1, bax-alpha, bcl-2, bak, bik, bcl-x), as well as a beta-actin control. A positive control RNA is also included. The beta-actin primer is a useful internal control for sample quantification and for comparison between samples. PCR products generated from the positive control RNA can be distinguished from target mRNA due to size differences of the amplified products.
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做完实验之后放8联排的卡槽怎么按都弹不出来了。求助各位可能的原因有哪些?原因越详细越多越好,避免下次再出现这种状况,有没有其他战友遇到过这种情况?谢谢!
国产的我查了博日的,也要三五万,最小的也要3万。这是不是pcr的普遍报价阿,觉得好贵呀。
国产的质量如何?还有其他可靠的厂家吗?国产的对实验影响会有多大呀?
请问PCR仪设置加样量有什么作用,我们实验室有的pcr仪可以设置有的不能设置。如果我用了25微升体系,pcr仪设置时设置了50微升体系会怎么样呢?帮忙回答一下。谢谢~
普通PCR的孔所有温度是一样的。

