The Bcl-2 gene family encodes a set of proteins involved in apoptotic inhibition, which are commonly analyzed during the performance of a Bcl-2 apoptosis assay. The ApoPrimer Set (Bcl-2 family) for RT-PCRis composed of a set of primers used toamplify cDNA derived from the mRNA of seven Bcl-2 family members (mcl-1, bfl-1, bax-alpha, bcl-2, bak, bik, bcl-x), as well as a beta-actin control. A positive control RNA is also included. The beta-actin primer is a useful internal control for sample quantification and for comparison between samples. PCR products generated from the positive control RNA can be distinguished from target mRNA due to size differences of the amplified products.
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2、放 PCR 离心管,先把顶盖向上扳,再往前推,露出放样孔,PCR 管
放入前应加好反应体系各组分,再放入 PCR 离心管。
3、反向重复第二步骤将顶盖板向后拉,盖好顶盖。
4、按 F2“Create”新建一个扩增的 PCR 程序。
5、按控制台面右方上下左右方向键,可随意移动编辑位置,输入需要
的温度、时间、循环数等。
6、据 PCR 离心管中加入的反应体积总量,在“Reaction volume”后输入
相应数值,有 Std“1~100ul”和 9600“1~50ul”两档可供选择。
7、按 F1“Start”启动
8、按“INFO”对应键查看 PCR 结束键。
9、扩增完成后按台面左方“Stop”键退出。
10、按“Hist”,可查看上次扩增的历史记录。11、完全退出后,按台面左下方电源开关,拔出插销,切断电源。
国产的我查了博日的,也要三五万,最小的也要3万。这是不是pcr的普遍报价阿,觉得好贵呀。
国产的质量如何?还有其他可靠的厂家吗?国产的对实验影响会有多大呀?

