
The Bcl-2 gene family encodes a set of proteins involved in apoptotic inhibition, which are commonly analyzed during the performance of a Bcl-2 apoptosis assay. The ApoPrimer Set (Bcl-2 family) for RT-PCRis composed of a set of primers used toamplify cDNA derived from the mRNA of seven Bcl-2 family members (mcl-1, bfl-1, bax-alpha, bcl-2, bak, bik, bcl-x), as well as a beta-actin control. A positive control RNA is also included. The beta-actin primer is a useful internal control for sample quantification and for comparison between samples. PCR products generated from the positive control RNA can be distinguished from target mRNA due to size differences of the amplified products.
ebiomall.com






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普通PCR的孔所有温度是一样的。
谢谢!
PCR技术类似于DNA的天然复制过程,其特异性依赖于与靶序列两端互补的寡核苷酸引物,由变性--退火--延伸三个基本反应步骤构成
各位大神,有使用过ThermoPX2这个型号的pcr扩增仪吗?

