
Product Highlights
- Sensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets
- Reproducible – consistent results between technical replicates for increased confidence in results
- Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
- Robust – reliable detection of RNA targets from a broad range of sample types
- Fast – delivers reproducible, accurate assay results in as little as 40 minutes
Product Description
The SensiFAST™ SYBR® Hi-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube.
An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation.
The SensiFAST SYBR® Hi-ROX One-Step Kit has been validated on all commonly-used real-time instruments that require a high concentration of the passive reference dye ROX.
Applications
- Gene expression analysis
- Pathogen detection
- RNA viral pathogen detection
- Genetic profiling
- miRNA profiling / quantification
- Genetically modified organisms (GMO) characterization

SensiFAST Products Customer Review
SensiFAST SYBR No-ROX One-Step Kit Customer Review
Real-Time PCR Selection Chart
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荧光原位杂交技术(Florescence In-Situ Hybridization简称FISH)是一种利用非放射性的荧光信号对原位杂交样本进行检测的技术。它将荧光信号的高灵敏度、安全性,荧光信号的直观性和原位杂交的高准确性结合起来,通过荧光标记的DNA探针与待测样本的DNA进行原位杂交,在荧光显微镜下对荧光信号进行辨别和计数,从而对染色体或基因异常的细胞、组织样本进行检测和诊断,为各种基因相关疾病的分型、预前和预后提供准确的依据。自20世纪80年代末,Pinkel和Heiles将FISH技术引入染色体检测领域后,FISH技术就在临床诊断及科研工作中得到了广泛的运用,显示出与一些传统技术相比的明显优势。
与传统的免疫组织化学法(IHC)相比,FISH具有良好的稳定性和可重复性。目前免疫组织化学法正广泛应用于肿瘤等多个领域的临床诊断。免疫组化的检测对象是疾病相关的蛋白。由于蛋白的表达和本身的构象受各种因素的影响很大(例如酸、碱和变性剂),检测条件的稳定性对检测结果至关重要。此外,免疫组化检测结果的判断依赖于检测者对显色结果的主观判断,对于一些弱阳性的结果,不同的检测者容易产生分歧。上述的因素都可能影响医生对病情的最终诊断。荧光原位杂交技术检测的对象是细胞中的DNA,致密的双螺旋结构使DNA可以历经千百万年而依然保持良好,其结构稳定,不易被环境条件影响,为荧光原位杂交技术的稳定提供了良好的基础。此外,荧光原位杂交结果的判定借助于对荧光的颜色判断和信号计数,客观地量化了检测地结果。如果借助于相应的FISH操作系统(例如Abbott-Vysis公司的Hybrit杂交仪和VP2000样本预处理系统)和染色体成像系统(例如AI公司的CytoVision)就能实现整个FISH操作的自动化,最大限度的降低操作者和检测者的主观因素,确保结果的准确性。
PCR由于灵敏度高,操作简便快捷是近年来应用比较多的基因诊断技术,但PCR诊断技术的假阴性和假阳性的比例较高,对同一样本也只能作一次分析,不能重复实验结果。随着探针技术的不断发展,FISH目前的灵敏度已经接近或达到PCR的水平,并能很好弥补PCR技术的局限。FISH技术不仅有极低的假阳性、假阴性的比例(以Abbott-Vysis的产前诊断探针为例,29,000例的使用结果证实正确率高达99.9%),还能对同一样本进行多次的FISH操作以及利用不同颜色的荧光探针一次检测多个染色体或基因的异常,大大节省了检测的时间。此外,通过FISH可以对染色体或特定基因的数目异常,特定片断的缺失、易位和重排进行诊断研究。展开
那个没有杂交仪的话,是要怎么搞?各位前辈,请指教。。。。。感激涕零

