
- SynonymTransferrin,TF,DKFZp781D0156,PRO1557,PRO2086
- SourceMouse Transferrin, Mouse IgG2a Fc Tag, low endotoxin (TRN-M525b) is expressed from human 293 cells (HEK293). It contains AA Val 20 - His 697 (Accession # Q921I1-1).Predicted N-terminus: Val 20Request for sequence
- Molecular Characterization
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 101.8 kDa. The protein migrates as 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 0.1 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.

Mouse Transferrin, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized Human TfR, His Tag (Cat. No. CD1-H5243) at 2 μg/mL (100 μL/well) can bind Mouse Transferrin, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. TRN-M525b) with a linear range of 0.4-12.5 ng/mL (QC tested).
- BackgroundTransferrin is also known as Serotransferrin, Beta-1 metal-binding globulin, TF, and is iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). The affinity of transferrin for Fe(III) is extremely high (1023 M−1 at pH 7.4) but decreases progressively with decreasing pH below neutrality.When not bound to iron, it is known as "apo-transferrin”. In humans, transferrin consists of a polypeptide chain containing 679 amino acids. It is a complex composed of alpha helices and beta sheets to form two domains (the first situated in the N-terminus and the second in the C-terminus). The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-binding site. The liver is the main source of manufacturing transferrin, but other sources such as the brain also produce this molecule . Transferrin is also associated with the innate immune system. Transferrin is found in the mucosa and binds iron, thus creating an environment low in free iron that impedes bacteria survival in a process called iron withholding. The level of transferrin decreases in inflammation. The metal binding properties of transferrin have a great influence on the biochemistry of plutonium in humans. Transferrin has a bacteriocidal effect on bacteria, in that it makes Fe3+ unavailable to the bacteria.Carbohydrate deficient transferrin increases in the blood with heavy ethanol consumption and can be monitored via laboratory testing.
- References
- (1)Crichton RR, Charloteaux-Wauters M. 1987, Eur. J. Biochem. 164 (3): 485–506.
- (2)Aisen, Phillip, et al.,1978, Journal of Biological Chemistry 253 (6): 1930–1937.
- (3)Ritchie RF,et al., 1999, J. Clin. Lab. Anal. 13 (6): 273–9.
- (4)Sharpe PC, 2001, Ann. Clin. Biochem. 38 (Pt 6): 652–64.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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缺点也很多。激光的功率很小,对荧光信号的灵敏度和分辨率都很差。只有很强的信号才适合于在这个机器上使用。如果经常要改变染色的步骤,使用不同的样本,或者抗原的量不确定,不建议用这个机器。
在流式细胞仪中,用PI单染法区分死细胞和活细胞,怎么看数据结果?
不过还要考虑很多因素:标记的方法会不会影响细胞下面的培养细胞是否能够耐受长时间接触房间中的氧气和二氧化碳的浓度机器的无菌情况
(溶血):红细胞,血红蛋白分解,红细胞溶解逃逸所述,称为溶血。通过各种物理和化学因素和毒素。在体外,如低渗溶液中,强烈的机械振荡,突然冷冻(-20℃-25℃)或突然化冻,过酸或过碱,以及乙醇,乙醚,皂碱,胆碱盐可引起溶血。人血浆的等渗溶液为0.9%NaCl溶液,红细胞在低于0.45%的NaCl溶液中,由于水的渗透,肿胀和红细胞破裂,血红蛋白逸出。在体内,溶血溶血性细菌侵入或某些毒素的抗原 - 抗体反应(如输入配血不合的血液),各种机械性损伤,红细胞内在(膜,酶)引起某些药物的缺陷。溶血性细菌,如某些溶血性链球菌和产气荚膜梭菌可导致败血症。的红血细胞和某些溶血性毒液含酶卵磷脂,卵磷脂血浆或红细胞成溶血卵磷脂,使红细胞膜分解疟原虫破坏。
由于研究的就没有限制了。
举几个例子:
96孔盘单个细胞分选,目标细胞只要96个。如果阳性率是50%,那几百个细胞就可以了,如果阳性率不到1%,那几万个细胞也不一定能选出来。
或者,阳性率有20-30%,但是下游实验需要几百万个细胞,那你需要几千万的细胞才可以满足。
类似于天文学上观察星星。光线从星球的背后照射过来。FSC看的是激光照射细胞后,从四周散发的光线,越大的细胞光晕越大。信号也越强
而一些老式 的机器,由于装备的激光数量和检测器都很有限,比如BD calibur这款机器。最高配置也就是蓝色(488nm)和红色(647nm)两种激光。检测4种颜色(通道)。所以为方便起见,就命名为FL1,FL2, FL3和FL4。前三种由于蓝色激光,FL4用于检测红色激光所激发的荧光。其中FL1是检测绿色荧光的(比如FITC,荧光素或者绿色荧光蛋白),而FL2是检测橙色荧光的(比如PI染色)。
过表达质粒转染细胞,以空载质粒做对照,48h后检测对凋亡的影响。请问定象限的时候是用空载质粒的对照组,还是需要用未转染的正常细胞?

