- SynonymFIGF,VEGFD
- SourceHuman VEGF-D, His Tag (VED-H5228) is expressed from human 293 cells (HEK293). It contains AA Phe 93 - Ser 201 (Accession # AAH27948).Predicted N-terminus: Phe 93Request for sequence
- Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 13.4 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.

Human VEGF-D, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) at 5 μg/mL (100 μL/well) can bind Human VEGF-D, His Tag (Cat. No. VED-H5228) with a linear range of 0.08-0.6 μg/mL (QC tested).
- BackgroundVascular endothelial growth factor D (VEGF-D) is also known as C-fos induced growth factor (FIGF), which belongs to the PDGF / VEGF growth factor family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. This secreted protein VEGF-D / FIGF undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-2 and VEGFR-3. The structure and function of this protein is similar to those of VEGFC. FIGF / VEGF-D is highly expressed in lung, heart, small intestine and fetal lung. FIGF / VEGF-D may function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR / FLK1) and VEGFR-3 (FLT4) receptors.
- References
- (1)Leppanen V.M., et al., 2011, Blood 117:1507-1515.
- (2)Yamada Y., et al., 1997, Genomics 42 (3): 483–8.
- (3)Achen MG., et al., 1998, Proc. Natl. Acad. Sci. U.S.A. 95 (2): 548–53.
- (4)Yasuoka H., et al., 2005, Mod. Pathol. 18 (8): 1127–33.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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比较常用的是用计数微球。比如临床上的CD4细胞绝对计数,就是采用这个方法。试管内事先已经有固定数目的计数微球和染色抗体。按要求加入固定体积的抗凝血液,裂解红细胞后上机。记录一千个微球,同时就会得到不同染色区域细胞的数量。这样就可以算出单位体积血液内CD4细胞的绝对数目了。
这样的计数微球也可以另外购买。浓度是固定的。在你已知体积的细胞悬液中加入一定体积的微球后上机,记录一千个微球的数据,细胞的计数也会同时记录。根据微球的浓度就可以推算出细胞的浓度,从而得出绝对计数了
另外一个方法是有些流式细胞仪是使用微泵加样,而不是连续吸取样本,这样样本的体积是已知的,记录一次就是所有样本的数量,可以算出细胞浓度
而一些老式 的机器,由于装备的激光数量和检测器都很有限,比如BD calibur这款机器。最高配置也就是蓝色(488nm)和红色(647nm)两种激光。检测4种颜色(通道)。所以为方便起见,就命名为FL1,FL2, FL3和FL4。前三种由于蓝色激光,FL4用于检测红色激光所激发的荧光。其中FL1是检测绿色荧光的(比如FITC,荧光素或者绿色荧光蛋白),而FL2是检测橙色荧光的(比如PI染色)。
举几个例子:
96孔盘单个细胞分选,目标细胞只要96个。如果阳性率是50%,那几百个细胞就可以了,如果阳性率不到1%,那几万个细胞也不一定能选出来。
或者,阳性率有20-30%,但是下游实验需要几百万个细胞,那你需要几千万的细胞才可以满足。
由于研究的就没有限制了。
在流式细胞仪中,用PI单染法区分死细胞和活细胞,怎么看数据结果?

