
- SynonymFIGF,VEGFD
- SourceHuman VEGF-D, His Tag (VED-H5228) is expressed from human 293 cells (HEK293). It contains AA Phe 93 - Ser 201 (Accession # AAH27948).Predicted N-terminus: Phe 93Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 13.4 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.

Human VEGF-D, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) at 5 μg/mL (100 μL/well) can bind Human VEGF-D, His Tag (Cat. No. VED-H5228) with a linear range of 0.08-0.6 μg/mL (QC tested).
- BackgroundVascular endothelial growth factor D (VEGF-D) is also known as C-fos induced growth factor (FIGF), which belongs to the PDGF / VEGF growth factor family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. This secreted protein VEGF-D / FIGF undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-2 and VEGFR-3. The structure and function of this protein is similar to those of VEGFC. FIGF / VEGF-D is highly expressed in lung, heart, small intestine and fetal lung. FIGF / VEGF-D may function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR / FLK1) and VEGFR-3 (FLT4) receptors.
- References
- (1)Leppanen V.M., et al., 2011, Blood 117:1507-1515.
- (2)Yamada Y., et al., 1997, Genomics 42 (3): 483–8.
- (3)Achen MG., et al., 1998, Proc. Natl. Acad. Sci. U.S.A. 95 (2): 548–53.
- (4)Yasuoka H., et al., 2005, Mod. Pathol. 18 (8): 1127–33.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
取对数生长期的细胞,按1X106个/mL以1mL体积接种于6孔板内。培养24h后,加入不同浓度样品。在37 ℃、5 % 的CO2恒温箱中培养24h后终止。
(2)细胞固定
胰酶消化并离心收集细胞(800rpm/min),弃上清,用预冷PBS洗细胞两次,加入预冷70%乙醇, -20℃固定保存。
(3) 细胞染色
离心收集细胞(800rpm/min),以1mL的PBS洗细胞一次,加入500uLPBS含50ug/mL溴化乙锭(PI),100ug/mL RNase A, 4℃避光孵育20分钟。
(4)检测
以标准程序用流式细胞仪检测,汞激发波长488nm,计数8千个细胞,结果用软件WinMDI Version 2.9分析。
比如白血病,骨髓瘤,艾滋病治疗的监测(看CD4细胞的水平)等等。
流失细胞仪检测细胞数量,可能会出现红色的结果吗?
我有过两台,一台配有三色激光,当时的报价将近40万美元,最近买的另外一台,两个激光,30万美元。
类似于天文学上观察星星。光线从星球的背后照射过来。FSC看的是激光照射细胞后,从四周散发的光线,越大的细胞光晕越大。信号也越强
正常人T细胞占到淋巴细胞的60-90%。T细胞一般有分为CD4+ 和CD8+这两种,两者的比例正常范围是0.9-6.

