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Addgene/pAAV-CAG-GFP/1unit/37825-AAV1

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Addgene

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37825-AAV1

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Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	37825	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart
AAV1	37825-AAV1	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV1 trial size	37825-AAV1.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV2	37825-AAV2	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV2 trial size	37825-AAV2.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV5	37825-AAV5	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV5 trial size	37825-AAV5.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV8	37825-AAV8	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV8 trial size	37825-AAV8.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV9	37825-AAV9	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV9 trial size	37825-AAV9.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV Retrograde	37825-AAVrg	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV Retrograde trial size	37825-AAVrg.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV PHP.eB	37825-PHPeB	Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information		$380	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
AAV with CAG promoter
(Search Vector Database)
Backbone manufacturer
Scott Sternson
Backbone sizew/o insert(bp)4722
Vector type
Mammalian Expression, AAV ; Adeno-associated virus

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
30°C
Growth Strain(s)
NEB Stable
Growth instructions
Use recombinase-free E. coli (Stbl3, XL-10, Sure2 and DH5a et.al), grow at 30C
Copy number
Low Copy

Gene/Insert

Gene/Insert name
GFP
Alt name
Green fluorescent protein
Species
A. victoria
Insert Size (bp)
720
Mutation
N/A
PromoterCAG

Cloning Information

Cloning methodRestriction Enzyme
5′ cloning siteBamHI(not destroyed)
3′ cloning siteEcoRI(not destroyed)
5′ sequencing primeraaagagacagcaaccagg
3′ sequencing primercggcttctggcgtgtgac

Resource Information

Supplemental Documents
- pAAV-CAG-GFP.gb
Terms and Licenses
- UBMTA
- Ancillary Agreement for Plasmids Containing FP Materials
Industry Terms
- Not Available to Industry
Articles Citing this Plasmid
- 12 References

Depositor Comments

PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.

Information for AAV1 (Catalog # 37825-AAV1)(Back to top)

Purpose

Ready-to-use AAV1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV1
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV1 trial size (Catalog # 37825-AAV1.T)(Back to top)

Purpose

Ready-to-use AAV1 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV1
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV2 (Catalog # 37825-AAV2)(Back to top)

Purpose

Ready-to-use AAV2 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV2
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV2 trial size (Catalog # 37825-AAV2.T)(Back to top)

Purpose

Ready-to-use AAV2 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV2
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV5 (Catalog # 37825-AAV5)(Back to top)

Purpose

Ready-to-use AAV5 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV5
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV5 trial size (Catalog # 37825-AAV5.T)(Back to top)

Purpose

Ready-to-use AAV5 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV5
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV8 (Catalog # 37825-AAV8)(Back to top)

Purpose

Ready-to-use AAV8 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV8
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV8 trial size (Catalog # 37825-AAV8.T)(Back to top)

Purpose

Ready-to-use AAV8 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV8
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV9 (Catalog # 37825-AAV9)(Back to top)

Purpose

Ready-to-use AAV9 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV9
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV9 trial size (Catalog # 37825-AAV9.T)(Back to top)

Purpose

Ready-to-use AAV9 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV9
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV Retrograde (Catalog # 37825-AAVrg)(Back to top)

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV retrograde (AAVrg)
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene Comments

Retrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use.

Data submitted about 37825-AAVrg by requesting scientist(s):

Data 1: Guinea pig, Brain parenchyma

Information for AAV Retrograde trial size (Catalog # 37825-AAVrg.T)(Back to top)

Purpose

Ready-to-use AAV Retrograde trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV retrograde (AAVrg)
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene Comments

Retrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use.

Information for AAV PHP.eB (Catalog # 37825-PHPeB)(Back to top)

Purpose

Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
TiterEnter the titer here≥ 1×10¹³ vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, PHP.eB cap gene pUCmini-iCAP-PHP.eB (plasmid #103005)
BufferPBS + 0.001% Pluronic F-68
SerotypePHPeB (plasmid #103005)
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene Comments

Citation Information: When using the PHP.eB serotype in future publications, please acknowledge Viviana Gradinaru and cite Chan et al., Nat Neurosci, 20(8):1172-1179. Pubmed.

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售后申请： 耐心讲解优质服务蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时，您可通过我的订单提交您的“申请售后”，蚂蚁淘产品顾问会第一时间为您处理，在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线：4000-520-616。

第一届女神班电子竞技宣布JuMayumin离开团队

2019-04-22

自七十年代出现第一代流式细胞仪以来，随着计算机技术、电子制造技术、激光技术及荧光素合成技术的不断发展，现代流式细胞仪已今非昔比，制造工艺、功能、精确度有了质的飞跃。<br>流式细胞仪生产厂商推出各种不同型号的流式细胞仪来满足用户的不同需要。现生产流式细胞的厂商全球至少有六家，各厂商产品又有不同的系列，各具特点。如何从众多的型号中挑选出最适合自己的机型，我们还需从分析应用出发，评估自己的需求来决定什么样的 ... 查看更多>

Alone [Anho](新人的FL Studio自制曲）_ (゜゜)つロ ...

2019-07-02

经过几年的攻坚，博奥生物集团有限公司自主研发完成的流式细胞仪，于6月22日正式取得医疗器械注册证（注册证编号：川械注准20162400136）。该仪器的开发获得了国家重大科学仪器设备开发专项项目支持，这次注册的流式细胞仪共包括四种机型：CytoNova1030，CytoNova2040，CytoNova2050，CytoNova2060，可分别满足临床和科研各种用途的需求。流式细胞仪以细胞悬液中的细胞或细胞器为对象，快速测定细胞的物理特... 查看更多>

超高速的时间戳A50micro Apogee流式细胞仪报价/价格普瑞麦迪(

2020-03-05

香港伯齐科技有限公司在发布的Apogee A50 Micro流式细胞仪供应信息，浏览与Apogee A50 Micro流式细胞仪相关的产品或在搜索更多与Apogee A50 Micro流式细胞仪相关的内容。查看更多>

Dabiré Oumar | Daccat Canhễt | व्यक्तिहरूको सूची

2019-02-02

抗体/蛋白/试剂盒 Abbiotec,Abcam,Abd Serotec,Abnova,ADI,Antibodies-online,AssayPro,Biorbyt,Biovision,BTI,Cellgro,CST,Chromotek,Covance,Echelon,Endocrine Tech... 查看更多>

【北京浩然正康科技有限公司招聘信息】_猎聘 liepin.com

2018-07-19

流式细胞仪（flow cytometer）是一种能够探测和计数以单细胞液体流形式穿过激光束的细胞检测装置，由于在检测中使用的细胞标志示踪物质为荧光标记物，因此，用来分离、鉴定细胞的流式细胞仪有被称为荧光激活细胞分类仪，是分离和鉴定细胞群及亚群的一种强而有力的应用工具。查看更多>

碱性磷酸酶(ALP)检测试剂盒使用说明

2020-01-28

深圳市百力特生物医疗科技有限公司在发布的流式细胞仪检测服务供应信息，浏览与流式细胞仪检测服务相关的产品或在搜索更多与流式细胞仪检测服务相关的内容。查看更多>

Novatein生物代理品牌:NovateinNovatein网

2021-07-24

Navios流式细胞仪在美国和中国喜获诊断使用批准查看更多>

通用电气将收购贝克休斯交易价值或将超200亿美元美股股 ...

2021-08-11

Partec流式细胞仪农林口学术会议partec流式细胞仪目前在植物和水产育种得到了广泛的应用和认可，其中中国农业科学院柑桔研究所（西南大学柑桔研究所）用partec流式细胞仪筛选出大量单倍体，打破了育种界一直认为的“柑橘无单倍体的”说法。中柑所在线讯：中柑所科研人员通过半年多来的不懈探索，掌握了柑桔染色体倍性快速检测技术，以及单倍体实生苗培育与加倍技术，成功获得一批纯系二倍体与纯系四倍体材料。柑桔作为多年生木本作物，童期长，遗传背景复... 查看更多>

液体菌种摇床培养方法与技术

2018-03-20

碧迪医疗器械（上海）有限公司(BD)在发布的流式临床检验新标准分析型流式细胞仪BD FACSCanto至尊版供应信息，浏览与流式临床检验新标准分析型流式细胞仪BD FACSCanto至尊版相关的产品或在搜索更多与流式临床检验新标准分析型流式细胞仪BD FACSCanto至尊版相关的内容。查看更多>

固特异工艺(GoodyearWelted)_

2019-06-17

贝克曼库尔特FC 500 五色数字化流式细胞仪是由默瑞（上海）生物科技有限公司代理或销售的贝克曼品牌的仪器，产品来源于德国。默瑞（上海）生物科技有限公司是中国最权威的贝克曼库尔特FC 500 五色数字化流式细胞仪销售服务商之一，在上海等地方销售贝克曼库尔特FC 500 五色数字化流式细胞仪已经多年。同时，生物在线为您提供众多企业贝克曼库尔特FC 500 五色数字化流式细胞仪仪器产品及图片，以便挑选到性价比高，合适的贝克曼库尔特FC 500 五色数字化流式细胞仪产品查看更多>

[求助]AMV和MMLV的特点对比核酸基因技术

2018-09-09

碧迪医疗器械（上海）有限公司(BD)在发布的BD Accuri C6 个人型流式细胞仪供应信息，浏览与BD Accuri C6 个人型流式细胞仪相关的产品或在搜索更多与BD Accuri C6 个人型流式细胞仪相关的内容。查看更多>

教育部厦门大学单一来源采购Chembridge化合物库征求意见 ...

2019-06-18

深圳中邦国际工程科技顾问有限公司受中山大学附属第七医院（深圳）委托，根据《中华人民共和国政府采购法》等有关规定，现对流式细胞仪等设备一批采购（重新招标三）进行公开招标，欢迎合格的供应商前来投标。项目名称：流式细胞仪等设备一批采购（重新招标三）项目编号：COBO1904132519H01项目联系方式：项目联系人：乐小姐林小姐项目联系电话：33396389 采购单位联系方式：采购单位：中山大学附查看更多>

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蚂蚁淘的创始人兼CEO是钟定松先生，具有十年的从业经验，在业界享有良好的口碑； Ebiomall是跨境直采平台，我们直接从厂家采购，自己的团队负责国际物流和清关，中间没有第三方，蚂蚁淘承诺所售产品仅为正品，假一罚十。

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商品咨询

交通学院123

ftzh5132017-10-05

和传统意义的流式细胞仪是有区别的。本质上就是一个非常简化了的流式细胞仪，只能区别大小和细胞的颗粒程度。没有其他荧光通道

刚学流式细胞仪,还不太会分析,求帮助_问答详情_流式细胞仪_计数...123

A雅雅雅2021-08-11

做的是淋巴细胞分离，然后用流式细胞仪检测，出来了结果，但是不太会看，求帮助

请教一下国内外有哪些生产流式细胞仪的公司以及详细信息123

2017-10-23

最好有详细一点信息，比如地址，总机，网址，是否有研发，谢谢

如何运用流式细胞仪进行T淋巴细胞亚群分析123

A雅雅雅2021-07-24

刚刚学的流式细胞仪，做了实验，可是拿到结果却完全看不懂求助大神

市场上常见流式数据分析软件和flowjo之比较.pdf原创力文档123

凝帝系列562aDw2017-11-04

Flowjo可以读取FCS格式的文件，一般的流式细胞仪都产生这个格式的文件。把FCS文件导入Flowjo后，双击导入的文件，默认打开的是FSC，SSC 点状图，你可以根据具体的使用更换坐标，或者设定gate

流式细胞仪检测细胞周期操作步骤123

2017-10-05

在细胞培养液中加入模拟核苷酸EdU （比较老的方法也可以用BrdU）。培养一小时后，更换为正常的培养液继续培养。模拟核苷酸可以像天然核苷酸一样被细胞摄取并整合入新合成的DNA。
培养一段时间后（一般小于一个细胞周期），固定细胞。根据所使用模拟核苷酸的不同，使用不同的方法。EdU，改变缓冲液的pH值，就会发出荧光。而BrdU则需用荧光抗体识别，需要对细胞膜和核膜进行通透处理。
再加入PI对DNA染色。
在二维坐标图上，横坐标设为线性PI荧光强度，纵坐标设为模拟核苷酸的荧光强度（log）。
典型的图就像下面一样：
G1, G2 和S期就按照图中的划分来做。G1期的细胞没有新合成DNA，所以BrdU信号是阴性，而PI的信号位于1倍体期。S期是正在合成DNA，所以BrdU都是阳性。G2期是已经合成好了2倍的DNA，所以位于2倍体期，信号是G1期的一倍。这个图中，G1 PI信号是300， G2就是600.

13 流式细胞仪检测细胞凋亡操作流程123

火一样2021-08-05

最近使用流式细胞仪，听到管理仪器的人说流式细胞仪的技术不就是调电压吗？表示很无奈，自己作为新手，很多也都不会，RAW和hela细胞的凋亡检测也不会，raw之前有人帮我调了一下，感觉还好，可是hela细胞就是fsc和ssc的二维图中的点很分散，圈细胞群后（control组不知为啥分两群了，实验组很分散）。用索莱宝的凋亡检测试剂盒检测后，不知为啥图中的细胞形成一条线。实验结果基本没用。现在快失去科研兴趣了，早上五点多起来做实验，晚上也没吃晚饭，哎，，想哭！

如下图流式细胞仪的横坐标是什么意思_问答详情_蚂蚁淘,【正品...123

强力杰2021-07-20

横坐标是DNA染料PI收到波长为488nm的激光激发后所放出的荧光强度。

流式细胞仪（Flow cytometry ）是对细胞进行自动分析和分选的装置。它可以快速测量、存贮、显示悬浮在液体中的分散细胞的一系列重要的生物物理、生物化学方面的特征参量，并可以根据预选的参量范围把指定的细胞亚群从中分选出来。多数流式细胞计是一种零分辨率的仪器，它只能测量一个细胞的诸如总核酸量，总蛋白量等指标，而不能鉴别和测出某一特定部位的核酸或蛋白的多少。也就是说，它的细节分辨率为零。

bd流式细胞仪常用试剂与耗材 123

晚晚_WddG22017-10-23

耗材：流式的试管细胞滤网加样的吸管枪头试剂：
染色的各种染料，标记好的荧光抗体染色所需要的缓冲液，封闭液，PBS最终由于上样的缓冲液

经流式细胞仪分离出的细胞可继续培养董阳答案网123

kaiyue12112017-10-24

当然可以。有细胞分选功能的流式细胞仪就是为了这个设计的。
不过还要考虑很多因素：标记的方法会不会影响细胞下面的培养细胞是否能够耐受长时间接触房间中的氧气和二氧化碳的浓度机器的无菌情况

2016版流式细胞仪选购指南[选购宝典]123

2017-10-24

红细胞溶血

（溶血）：红细胞，血红蛋白分解，红细胞溶解逃逸所述，称为溶血。通过各种物理和化学因素和毒素。在体外，如低渗溶液中，强烈的机械振荡，突然冷冻（-20℃-25℃）或突然化冻，过酸或过碱，以及乙醇，乙醚，皂碱，胆碱盐可引起溶血。人血浆的等渗溶液为0.9％NaCl溶液，红细胞在低于0.45％的NaCl溶液中，由于水的渗透，肿胀和红细胞破裂，血红蛋白逸出。在体内，溶血溶血性细菌侵入或某些毒素的抗原 - 抗体反应（如输入配血不合的血液），各种机械性损伤，红细胞内在（膜，酶）引起某些药物的缺陷。溶血性细菌，如某些溶血性链球菌和产气荚膜梭菌可导致败血症。的红血细胞和某些溶血性毒液含酶卵磷脂，卵磷脂血浆或红细胞成溶血卵磷脂，使红细胞膜分解疟原虫破坏。

BCAA123

sen37992017-10-24

可以的，目前根据机器的型号不同，可以有两种方法。
比较常用的是用计数微球。比如临床上的CD4细胞绝对计数，就是采用这个方法。试管内事先已经有固定数目的计数微球和染色抗体。按要求加入固定体积的抗凝血液，裂解红细胞后上机。记录一千个微球，同时就会得到不同染色区域细胞的数量。这样就可以算出单位体积血液内CD4细胞的绝对数目了。

这样的计数微球也可以另外购买。浓度是固定的。在你已知体积的细胞悬液中加入一定体积的微球后上机，记录一千个微球的数据，细胞的计数也会同时记录。根据微球的浓度就可以推算出细胞的浓度，从而得出绝对计数了

另外一个方法是有些流式细胞仪是使用微泵加样，而不是连续吸取样本，这样样本的体积是已知的，记录一次就是所有样本的数量，可以算出细胞浓度