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Boston Biochem/LY 294002 hydrochloride/1130/5
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4000-520-616
Biological Activity Technical DataAdditional InformationBackground Product DatasheetsCalculators
Biological Activity
Prototypical PI 3-kinase inhibitor (IC50 values are 0.31, 0.73, 1.06 and 6.60 μM for PI 3-Kβ, PI 3-Kα, PI 3-Kδ and PI 3-Kγ respectively). Also inhibits other kinases including, CK2, mTOR, PLK1, PIM1 and PIM3. Inhibits proliferation and induces apoptosis in human colon cancer cells in vitro and in vivo. Suppresses proliferation of mESCs. Inhibits autophagic sequestration in rat hepatocytes.
Negative Control also available.
Technical Data
M.Wt:
343.81
Formula:
C19H17NO3.HCl
Solubility:
Soluble to 25 mM in DMSO and to 5 mM in ethanol
Purity:
≥99%
Storage:
Store at RT
CAS No:
934389-88-5
The technical data provided above is for guidance only.For batch specific data refer to the Certificate of Analysis.Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
Other Product-Specific Information:
- Negative Control Available
- View R&D Systems' research posterfeaturing LY 294002 hydrochloride
Background References
- The selectivity of protein kinase inhibitors: a further update.Bain et al.Biochem.J., 2007;408:297
- Phosphoinositide 3-kinase inhibitor LY294002 but not serum withdrawal suppresses proliferation of murine embryonic stem cells.Lianguzova et al.Cell Biol.Int., 2007;31:330
- The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibit autophagy in isolated rat hepatocytes.Blommaart et al.Eur.J.Biochem., 1997;243:240
- A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY 294002).Vlahos et al.J.Biol.Chem., 1994;269:5241
Product Datasheets
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Citations for LY 294002 hydrochloride
The citations listed below are publications that use Tocris products.Selected citations for LY 294002 hydrochloride include:
115Citations: Showing 1 - 10
- Extracorporeal shock waves protect cardiomyocytes from DOX-induced cardiomyopathy by upregulating survivin via the integrin-ILK-Akt-Sp1/p53 axis.Authors: Lee Et al.Sci Rep2019;9:12149
- The standardized Korean Red Ginseng extract and its ingredient ginsenoside Rg3 inhibit manifestation of breast cancer stem cell-like properties through modulation of self-renewal signaling.Authors: Oh Et al.J Ginseng Res2019;43:421
- Dual RXR motifs regulate nerve growth factor-mediated intracellular retention of the delta opioid receptor.Authors: Shiwarski Et al.Mol Biol Cell2019;30:680
- Human vascular cell responses to the circulating bone hormone osteocalcin.Authors: Millar Et al.J Cell Physiol2019;234:21039
- The Protective Effect of Ins. on Rat Cortical Neurons in Oxidative Stress and Its Dependence on the Modulation of Akt, GSK-3beta, ERK1/2, and AMPK Activities.Authors: Zakharova Et al.Int J Mol Sci2019;20
- Characterization of the brain penetrant neuropeptide Y Y2 receptor antagonist SF-11.Authors: Domin Et al.ACS Chem Neurosci2019;10:3454
- Intracellular emetic signaling evoked by the L-type Ca2+ channel agonist FPL64176 in the least shrew (Cryptotis parva).Authors: Zhong Et al.Eur J Pharmacol2018;834:157
- The homozygous CX3CR1-M280 mutation impairs human monocyte survival.Authors: Collar Et al.JCI Insight2018;3
- PI3Kδ activates E2F1 synthesis in response to mRNA translation stress.Authors: Gnanasundram Et al.Nat Commun2017;8:2103
- A pluripotent stem cell-based model for post-implantation human amniotic sac development.Authors: ShaoNat Commun2017;8(1):208
- Structural and pharmacological basis for the induction of mitochondrial biogenesis by formo. but not clenbu.Authors: CameronSci Rep2017;7(1):10578
- CFTR-mediated anion secretion across intestinal epithelium-like Caco-2 monolayer under PTH stimulation is dependent on intermediate conductance K+ channels.Authors: Jantarajit Et al.Am J Physiol Cell Physiol2017;313:C118
- PI3K class II α regulates δ-opioid receptor export from the trans-Golgi network.Authors: Shiwarski Et al.Mol Biol Cell2017;28:2202
- Phosphoinositide-dependent enrichment of actin monomers in dendritic spines regulates synapse development and plasticity.Authors: Lei Et al.J Cell Biol2017;216:2551
- Signaling Cascade Involved in Rapid Stimulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by dexameth.Authors: Bossmann Et al.Int J Mol Sci2017;18
- Isolation and characterization of a new naturally immortalized human breast carcinoma cell line, KAIMRC1.Authors: Ali Et al.BMC Cancer2017;17:803
- Negative regulation of ERK1/2 by PI3K is required for the protective effects of Pyropia yezoensis peptide against perfluorooctane sulfonate-induced endoplasmic reticulum stress.Authors: Oh Et al.Mol Med Rep2017;15:2583
- Adenosine A3 receptor elicits chemoresistance mediated by multiple resistance-associated protein-1 in human glioblastoma stem-like cells.Authors: Torres Et al.Oncotarget2016;7:67373
- miR-382 targeting PTEN-Akt axis promotes liver regeneration.Authors: Bei Et al.Elife2016;7:1584
- The GABAB positive allosteric modulators CGP7930 and GS39783 stimulate ERK1/2 signalling in cellslacking functional GABAB receptorsAuthors: Olianas Et al.European Journal of Pharmacology2016;794:135
- Tumor suppression in basal keratinocytes via dual non-cell-autonomous functions of a Na,K-ATPase beta subunit.Authors: Hatzold Et al.Elife2016;5
- Cannabinoid receptor agonist WIN55,212-2 and fatty acid amide hydrolase inhibitor URB597 may protect against cognitive impairment in rats of chronic cerebral hypoperfusion via PI3K/AKT signalingAuthors: Shao-Hua Su Et al.Behavioural Brain Research2016;313:334
- Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal CellsAuthors: Mou Et al.Cell: Stem Cell2016;19:217
- Pyrrolidine dithiocarbamate activates the Nrf2 pathway in astrocytes.Authors: Liddell Et al.J Neuroinflammation2016;13:49
- Poly(I:C) increases the expression of mPGES-1 and COX-2 in rat primary microglia.Authors: Oliveira Et al.Int J Mol Med2016;13:11
- Progesterone receptor membrane component-1 regulates hepcidin biosynthesis.Authors: Li Et al.J Clin Invest2016;126:389
- Up-Regulated Expression of LAMP2 and Autophagy Activity during Neuroendocrine Differentiation of Prostate Cancer LNCaP CellsAuthors: Morell Et al.PLoS One2016;11:e0162977
- Phosphoinositide-3-Kinase Is the Primary Mediator of Phosphoinositide-Dependent Inhibition in Mammalian Olfactory Receptor Neurons.Authors: Ukhanov Et al.Front Cell Neurosci2016;10:97
- Drug-based modulation of endogenous stem cells promotes functional remyelination in vivo.Authors: Najm Et al.Int J Cancer2015;522:216
- Integrated β-catenin, BMP, PTEN, and Notch signalling patterns the nephron.Authors: Lindström Et al.J Biomed Sci2015;3:e04000
- The thymoquinone-induced production of reactive oxygen species promotes dedifferentiation through the ERK pathway and inflammation through the p38 and PI3K pathways in rabbit articular chondrocytes.Authors: Yu and KimAlcohol Clin Exp Res2015;35:325
- Acetate supplementation as a means of inducing glioblastoma stem-like cell growth arrest.Authors: Long Et al.J Cell Physiol2015;230:1929
- Dimeric dipeptide mimetics of the nerve growth factor Loop 4 and Loop 1 activate TRKA with different patterns of intracellular signal transduction.Authors: Gudasheva Et al.Nature2015;22:106
- PI3K Phosphorylation Is Linked to Improved Electrical Excitability in an In Vitro Engineered Heart Tissue Disease Model System.Authors: Kana Et al.Tissue Eng Part A2015;21:2379
- Neuregulin 1 signalling modulates mGluR1 function in mesencephalic dopaminergic neurons.Authors: Ledonne Et al.Mol Psychiatry2015;20:959
- A novel mechanism for cytoprotection against hypoxic injury: δ-opioid receptor-mediated increase in Nrf2 translocation.Authors: Cao Et al.Oncotarget2015;172:1869
- SDF-1α stiffens myeloma bone marrow mesenchymal stromal cells through the activation of RhoA-ROCK-Myosin II.Authors: Choi Et al.PLoS One2015;136:E219
- An in vitro model of latency and reactivation of varicella zoster virus in human stem cell-derived neurons.Authors: Markus Et al.PLoS Pathog2015;11:e1004885
- Upregulation of Nicotinic Acetylcholine Receptor α4+β2 through a Ligand-Independent PI3Kβ Mechanism That Is Enhanced by TNFα and the Jak2/p38Mapk Pathways.Authors: Rogers and GahringJ Neuroinflammation2015;10:e0143319
- Glucocorticoids Distinctively Modulate the CFTR Channel with Possible Implications in Lung Development and Transition into Extrauterine Life.Authors: Laube Et al.PLoS One2015;10:e0124833
- Palladin mediates stiffness-induced fibroblast activation in the tumor microenvironment.Authors: McLane and LigonBiophys J2015;109:249
- ER-α36-mediated rapid estrogen signaling positively regulates ER-positive breast cancer stem/progenitor cells.Authors: Deng Et al.BMC Cancer2014;9:e88034
- Angiogenic growth factors augment K-Cl cotransporter expression in erythroid cells via hypoxia-inducible factor-1α.Authors: Gonsalves Et al.Am J Hematol2014;89:273
- Non-cell-autonomous mechanism of activity-dependent neurotransmitter switching.Authors: Guemez-Gamboa Et al.Neuron2014;82:1004
- Interaction of mTOR and Erk1/2 signaling to regulate oligodendrocyte differentiation.Authors: Dai Et al.Glia2014;62:2096
- Rapid elevation of sodium transport through Ins is mediated by AKT in alveolar cells.Authors: Mattes Et al.Physiol Rep2014;2:e00269
- Decrease of PKB/Akt Phosphorylation is Partially Mediated by SAPK/JNK Activation in Serum-free L6 Myoblasts Starved with Low Glucose.Authors: Kim Et al.J Phys Ther Sci2014;26:1757
- δ-Opioid receptors up-regulate excitatory amino acid transporters in mouse astrocytes.Authors: Liang Et al.J Exp Bot2014;171:5417
- BDNF-endocannabinoid interactions at neocortical inhibitory synapses require phospholipase C signaling.Authors: ZhaoJ Neurophysiol2014;111:1008
- Characterisation of AmphiAmR11, an amphioxus (Branchiostoma floridae) D2-DA-like G protein-coupled receptor.Authors: Bayliss and EvansPLoS One2013;8:e80833
- Acetate supplementation induces growth arrest of NG2/PDGFRα-positive oligodendroglioma-derived tumor-initiating cells.Authors: Long Et al.PLoS One2013;8:e80714
- Curcumin suppresses migration and proliferation of Hep3B hepatocarcinoma cells through inhibition of the Wnt signaling pathway.Authors: Kim Et al.Mol Med Rep2013;8:282
- ATP-competitive mTOR kinase inhibitors delay plant growth by triggering early differentiation of meristematic cells but no developmental patterning change.Authors: Montané and MenandNeuroscience2013;64:4361
- PI3K-Akt signaling activates mTOR-mediated epileptogenesis in organotypic hippocampal culture model of post-traumatic epilepsy.Authors: Berdichevsky Et al.J Neurosci2013;33:9056
- Melatonin acts through MT1/MT2 receptors to activate hypothalamic Akt and suppress hepatic gluconeogenesis in rats.Authors: Faria Et al.Am J Physiol Endocrinol Metab2013;305:E230
- Cellular Ins resistance disrupts leptin-mediated control of neuronal signaling and transcription.Authors: Nazarians-Armavil Et al.Mol Endocrinol2013;27:990
- Label-free integrative pharmacology on-target of opioid ligands at the opioid receptor family.Authors: Morse Et al.BMC Pharmacol Toxicol2013;14:17
- Activation of AMPK inhibits cervical cancer cell growth through AKT/FOXO3a/FOXM1 signaling cascade.Authors: Yung Et al.J Steroid Biochem Mol Biol2013;13:327
- PI 3-kinase independent role for AKT in F-actin regulation during outer segment phagocytosis by RPE cells.Authors: Bulloj Et al.Exp Eye Res2013;113:9
- Melatonin induces transcriptional regulation of Bim by FoxO3a in HepG2 cells.Authors: Carbajo-Pescador Et al.Br J Cancer2013;108:442
- Ceramide 1-phosphate stimulates proliferation of C2C12 myoblasts.Authors: Gangoiti Et al.Biochimie2012;94:597
- Fucoidan from seaweed Fucus vesiculosus inhibits migration and invasion of human lung cancer cell via PI3K-Akt-mTOR pathways.Authors: Lee Et al.PLoS One2012;7:e50624
- PPARδ activation protects endothelial function in diabetic mice.Authors: Tian Et al.Diabetes2012;61:3285
- PI3K/Akt signaling pathway in the basolateral amygdala mediates the rapid antidepressant-like effects of trefoil factor 3.Authors: Shi Et al.Neuropsychopharmacology2012;37:2671
- Nucleus accumbens mGluR5-associated signaling regulates binge alcohol drinking under drinking-in-the-dark procedures.Authors: Cozzoli Et al.Cancer Res2012;36:1623
- Cervical spinal erythropoietin induces phrenic motor facilitation via extracellular signal-regulated protein kinase and Akt signaling.Authors: Dale Et al.J Neurosci2012;32:5973
- Curcumin inhibits HCV replication by induction of heme oxygenase-1 and suppression of AKT.Authors: Chen Et al.Int J Mol Med2012;30:1021
- A screen for regulators of survival of motor neuron protein levels.Authors: Makhortova Et al.Nat Chem Biol2011;7:544
- Ligand-directed functional selectivity at the mu opioid receptor revealed by label-free integrative pharmacology on-target.Authors: Morse Et al.PLoS One2011;6:e25643
- Astragaloside IV stimulates angiogenesis and increases hypoxia-inducible factor-1α accumulation via phosphatidylinositol 3-kinase/Akt pathway.Authors: Zhang Et al.J Pharmacol Exp Ther2011;338:485
- The canonical nuclear factor-κB pathway regulates cell survival in a developmental model of spinal cord motoneurons.Authors: Mincheva Et al.J Neurosci2011;31:6493
- JNK suppression is essential for 17β-OE inhibits prostaglandin E2-Induced uPA and MMP-9 expressions and cell migration in human LoVo colon cancer cells.Authors: Hsu Et al.J Med Food2011;18:61
- A positive cross-regulation of HER2 and ER-α36 controls ALDH1 positive breast cancer cells.Authors: Kang Et al.J Pharmacol Exp Ther2011;127:262
- Neurotoxicity induced by okadaic acid in the human neuroblastoma SH-SY5Y line can be differentially prevented by α7 and β2* nicotinic stimulation.Authors: Barrio Et al.Toxicol Sci2011;123:193
- TRPV4-mediated calcium influx into human bronchial epithelia upon exposure to diesel exhaust particles.Authors: Li Et al.Environ Health Perspect2011;119:784
- Cortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.Authors: Yip Et al.PLoS Biol2010;8:e1000399
- Extranuclear estrogen receptors mediate the neuroprotective effects of estrogen in the rat hippocampus.Authors: Yang Et al.PLoS One2010;5:e9851
- Pharmacological and genetic accumulation of hypoxia-inducible factor-1alpha enhances excitatory synaptic transmission in hippocampal neurons through the production of vascular endothelial growth factor.Authors: Huang Et al.J Neurosci2010;30:6080
- Leptin regulates AMPA receptor trafficking via PTEN inhibition.Authors: Moult Et al.J Neurosci2010;30:4088
- Mechano-transduction in osteoblastic cells involves strain-regulated estrogen receptor alpha-mediated control of Ins-like growth factor (IGF) I receptor sensitivity to Ambient IGF, leading to phosphatidylinositol 3-kinase/AKT-dependent Wnt/LRP5 receptor-iAuthors: Sunters Et al.J Biol Chem2010;285:8743
- miR-155 and its star-form partner miR-155* cooperatively regulate type I IF. production by human plasmacytoid dendritic cells.Authors: Zhou Et al.Blood2010;116:5885
- Level of G protein-coupled receptor kinase-2 determines myocardial ischemia/reperfusion injury via pro- and anti-apoptotic mechanisms.Authors: Brinks Et al.J Cell Sci2010;107:1140
- Optimal experimental design for parameter estimation of a cell signaling model.Authors: Bandara Et al.PLoS Comput Biol2009;5:e1000558
- FcεRI-induced activation by low antigen concentrations results in nuclear signals in the absence of degranulation.Authors: Grodzki Et al.Circ Res2009;46:2539
- Activation of glycogen synthase kinase-3 β is required for hyperDA and D2 receptor-mediated inhibition of synaptic NMDA receptor function in the rat prefrontal cortex.Authors: Li Et al.J Biol Chem2009;29:15551
- Regulation of p53 expression, phosphorylation and subcellular localization by a G-protein-coupled receptor.Authors: Solyakov Et al.Oncogene2009;28:3619
- PLZF is a regulator of homeostatic and cytokine-induced myeloid development.Authors: Doulatov Et al.Genes Dev2009;23:2076
- Glycogen synthase kinase-3 and Omi/HtrA2 induce annexin A2 cleavage followed by cell cycle inhibition and apoptosis.Authors: Wang Et al.Mol Biol Cell2009;20:4153
- Ethanol augments RANTES/CCL5 expression in rat liver sinusoidal endothelial cells and human endothelial cells via activation of NF-kappa B, HIF-1 alpha, and AP-1.Authors: Yeligar Et al.J Immunol2009;183:5964
- Ethanol-induced expression of ET-1 and ET-BR in liver sinusoidal endothelial cells and human endothelial cells involves hypoxia-inducible factor-1alpha and microrNA-199.Authors: Yeligar Et al.J Immunol2009;183:5232
- c-jun controls the ability of IL-12 to induce IL-10 production from human memory CD4+ T cells.Authors: Garcia Et al.J Immunol2009;183:4475
- C5a mediates peripheral blood neutrophil dysfunction in critically ill patients.Authors: Morris Et al.Am J Respir Crit Care Med2009;180:19
- Soy phytoestrogens are neuroprotective against stroke-like injury in vitro.Authors: Schreihofer and RedmondJ Neurosci2009;158:602
- Efficacy of signal pathway inhibitors alone and in combination with cisp. varies between human non-small cell lung cancer lines.Authors: Kilic Et al.J.Surg.Res.2009;154:9
- Blockade of estrogen receptor signaling inhibits growth and migration of medulloblastoma.Authors: Belcher Et al.Endocrinology2009;150:1112
- Mechanisms underlying fibronectin-induced up-regulation of P2X4R expression in microglia: distinct roles of PI3K-Akt and MEK-ERK signalling pathways.Authors: Tsuda Et al.J Cell Mol Med2009;13:3251
- Inulin increases glucose transport in C2C12 myotubes and HepG2 cells via activation of AMP-activated protein kinase and phosphatidylinositol 3-kinase pathways.Authors: Yun Et al.PLoS One2009;12:1023
- Synergistic effect of ERK inhibition on tetrandrine-induced apoptosis in A549 human lung carcinoma cells.Authors: Cho Et al.J Vet Sci2009;10:23
- PI3 kinase/Akt activation mediates estrogen and IGF-1 nigral DA neuronal neuroprotection against a unilateral rat model of Parkinson's disease.Authors: Quesada Et al.Dev Neurobiol2008;68:632
- Reactive oxygen species stabilize hypoxia-inducible factor-1 alpha protein and stimulate transcriptional activity via AMP-activated protein kinase in DU145 human prostate cancer cells.Authors: Jung Et al.Carcinogenesis2008;29:713
- Phosphatidylinositol 3-kinase is a key mediator of central sensitization in painful inflammatory conditions.Authors: Pezet Et al.J Neurosci2008;28:4261
- Temporal profiling of changes in phosphatidylinositol 4,5-bisphosphate, inositol 1,4,5-trisphosphate and diacylglycerol allows comprehensive analysis of phospholipase C-initiated signalling in single neurons.Authors: Nelson Et al.J Neurochem2008;107:602
- Hallucinogens recruit specific cortical 5-HT(2A) receptor-mediated signaling pathways to affect behavior.Authors: González-Maeso Et al.Exp Mol Med2007;53:439
- Resveratrol stimulates glucose transport in C2C12 myotubes by activating AMP-activated protein kinase.Authors: Park Et al.Br J Pharmacol2007;39:222
- cis-9,trans-11-conjugated linoleic acid down-regulates phorbol ester-induced NF-kappaB activation and subsequent COX-2 expression in hairless mouse skin by targeting IkappaB kinase and PI3K-Akt.Authors: Hwang Et al.Carcinogenesis2007;28:363
- Regulation of DA transporter function and cell surface expression by D3 DA receptors.Authors: Zapata Et al.J Biol Chem2007;282:35842
- IGF-I inhibition of apoptosis is associated with decreased expression of prostate apoptosis response-4.Authors: Chung Et al.J Endocrinol2007;194:77
- Ins induces airway smooth muscle contraction.Authors: Schaafsma Et al.J Biomed Sci2007;150:136
- PI-3-kinase-dependent membrane recruitment of centaurin-α2 is essential for its effect on ARF6-mediated actin cytoskeleton reorganisation.Authors: Venkateswarlu Et al.Neuron2007;120:792
- Cyclin D1 is necessary for tamoxifen-induced cell cycle progression in human breast cancer cells.Authors: Kilker and Planas-SilvaMol Immunol2006;66:11478
- Differential reelin-induced enhancement of NMDA and AMPA receptor activity in the adult hippocampus.Authors: Qiu Et al.Br J Pharmacol2006;26:12943
- Ischemic preconditioning protects by activating prosurvival kinases at reperfusion.Authors: Hausenloy Et al.Am J Physiol Heart Circ Physiol2005;288:H971
- Glucose deprivation increases mRNA stability of vascular endothelial growth factor through activation of AMP-activated protein kinase in DU145 prostate carcinoma.Authors: Yun Et al.J Neurosci2005;280:9963
- LY 294002 inhibits adenosine receptor activation by a mechanism independent of effects on PI-3 kinase or casein kinase II.Authors: Searl and SilinskyPurinergic Signal2005;1:389
- Phospholipase C-linked receptors regulate the ATP-sensitive potassium channel by means of phosphatidylinositol 4,5-bisphosphate metabolism.Authors: Xie Et al.Proc Natl Acad Sci U S A1999;96:15292
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PI3K inhibition decreased the LPA induced expression of pAMPK.
By lisha joshion 06/18/2019
Application: Species:Mouse
BV2 microglia cells were incubated for one hour with 10µM LY294002 prior to treatment with 1µM LPA. Western blot analysis revealed a possible role for PI3K in the LPA induced expression of pAMPK.
LY 294002 blocks LPA-mediated COX-2 expression
By Anonymouson 12/13/2018
Application: Species:Human
Human osteosarcoma cells were incubated with 10 μM LY 294002 for 30 min prior to treatment with 10 μM LPA to estimate COX-2 expression using Western blot analysis. LY 294002 abolished LPA-induced COX-2 expression.
LY 294002 inhibits 15d-PGJ2-induced Akt phosphorylation
By Christopher Trummeron 10/15/2018
Application: Species:Human
MG-63 cells were incubated with PD169316 (25 μM), LY294002 (10 μM) or Akt-1/2 (5 μM Akt-I) for 30 min prior to 15d-PGJ2 treatment (20 μM) for 1 h to follow pAkt expression using Western blot. Akt-1/2 completely blocked 15d-PGJ2-induced Akt phosphorylation in MG-63 cells.
Successfull PI 3-kinase inhibitor
By Anonymouson 01/09/2018
Application: Species:Human
I used LY294002 hydrochloride 10µM to inhibit pAKT response of FFAR GPCR activators (15min 37°C).
Reduction of Foxo1 phosphorylation in cultured theca cells by LY294002.
By Zhenmin Leion 01/04/2018
Application: Species:Mouse
Mouse ovarian theca cells isolated from 60-day old mice were used. Cultured Ptenfl/fl and tPtenMT ovarian theca cells were pretreated with vehicle (DMSO), LY294002 (50 mM), or API-2 (7.5 mM) for 1 h before the addition of LH (50 ng/mL) for 30 min. Western blotting was used to detect Foxo1 phosphorylation in cultured ovarian theca cells.
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A few cell types are thin enough to be viewed directly in a microscope (algae, protozoa, blood, tissue cultures), but most tissues (kidney, liver, brain) are t 查看更多
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2021-08-16
浅析CCD与CMOS影像感应器的技术原理介绍 CCD影像感应器目前已大部份被使用在数码相机上，而近年来CMOS感应器也逐渐开始出现在数码相机的市场当中，CMOS的诞生具备了许多CCD所没有的一些优势，例如：省电、高集成度、成本更低等等。因此就未来影像感应技术的发展来看，数码相机的影像感应器市场将会 查看更多
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2021-08-25
数码摄影的八大参数课程内容概要数码相机是集光学、机械、电子于一体的产品，它集成了影像信息的转换、存储、传输等部件，具有“数字化存取”模式、与电脑交互处理、实时拍摄等特点。数码相机的许多性能指标都借助了传统相机的相关概念，但数码相机与传统相机在构造上有着本质的不同，所以 查看更多
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数码相机的心脏——CCD 决定数码相机拍摄的画面质量高低的，从根本上来说是CCD。那么，只看CCD的性能，是否就能够了解相机的性能呢？回答是："从某种程度上来说，是的"。 由CCD的性能决定的图象性能的要素有很多，其中，能够为使用者得到相关信息的，包括以下几项：CCD尺寸、像素数量、单 查看更多
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2021-08-20
在PCHOME数码影象俱乐部论坛中，有关光学变焦和数码变焦的问题已经被网友们提出N多次了。大家对此问题都好象有了结论，没有什么可以再讨论的了。但仔细考虑，其中仍有不少有趣的东西值得我们去想一想。因此，我想在下面的文章里细说一下数码变焦以及与其相关的一些问题。让我们先看一个最简单 查看更多
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2021-09-21
Leica简史：35mm照相机的诞生在本世纪初，工业革命盛兴之际，人们所生产的工业产物无不以硕大、操作复杂为傲。同样地，当时的照相机亦是如此。木造的机身加上固定的镜头、快门无法调整速度、底片为大型的玻璃材料，无一不是体积庞大，却又操作困难的东西，这就是当时的相机。如果再加上当时摄 查看更多
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CRISPR打造强大成像系统123
论坛小文章2021-07-24
纽约大学的研究团队在CRISPR-Cas9的基础上开发了一个定向检测基因组区域的活体成像系统。该系统能够精确观测基因组位点和细胞核结构，揭示细胞核改变在基因表达调控和其他细胞过程中的重要作用。
CRISPR-Cas9原本是细菌在漫长的进化史中演化出的重要防御机制。规律成簇的间隔短回文重复CRISPR与内切酶Cas9的组合，可以在sgRNA的指引下，靶标并切割入侵者的遗传物质。2012年研究者们利用这一特点，将CRISPR系统发展成了强大的基因组编辑工具。现在CRISPR-Cas9基因组编辑系统的应用延伸到了基因敲除、删除、染色体重排、RNA编辑、全基因组筛选等众多领域。
研究人员用病毒RNA和sgRNA生成了嵌合转录本。这种转录本与缺乏剪切活性的Cas9共表达，可以把荧光标记的病毒RNA结合蛋白招募到基因组指定位点。为了证实CRISPR成像技术的效率和灵活性，研究人员同时标记了小鼠染色体12的两种卫星序列，以及不同的基因组位点。他们在文章中指出，这是一种快速、稳定的低背景成像技术，可以用来追踪染色质互作动态和验证表观遗传学过程。
为了更好的研究非编码RNA，哈佛大学的科学家们以CRISPR为基础打造出了一个定向的RNA定位法——CRISPR-Display（CRISP-Disp）。他们利用失去催化活性的dCas9，将整合在sgRNA中的大片段RNA带到特定DNA位点。文章通讯作者是RNA领域的著名青年科学家JohnRinn博士，他曾被评为2009年美国国内撼动科学界的青年英才。
在CRISPR系统的基础上使用sgRNA文库进行遗传筛选，是鉴定基因调控子的一种有效方法。研究者们可以通过CRISPR筛选在基因组非编码区域中寻找功能性元件。不过这样的应用需要高度覆盖又简单实用的自定义sgRNA文库。耶鲁大学医学院的研究人员开发了一个名为MolecularChipper的新技术。该技术能够针对指定基因组区域生成密集覆盖的sgRNA文库。
酿脓链球菌的Cas9现在已经被广泛用于基因组编辑。那么，对Cas9进行基因工程改造将面临哪些限制呢？加州大学伯克利分校的研究团队通过随机插入突变对Cas9结构进行了全面分析，鉴定了这种蛋白的基因改造热点。这些位点可以耐受PDZ结构域的插入，不影响Cas9的结合和剪切功能。
分析化学中几种常用的分析仪器 ppt课件123
▍Punk╮frr2018-01-14
根据我理解所说完美像应该指达衍射极限像质吧 近做反射式光系统理论单抛物面反射镜像质能达衍射极限且反射系统完全存色差 反射系统体积难控制且应用领域限
光系统设计难各种光系统都各自特点所像质优化重点全致 没像数或者物理领域种著名课题 希望能帮
光系统设计难各种光系统都各自特点所像质优化重点全致 没像数或者物理领域种著名课题 希望能帮
[图文]影像设备简介 123
2018-01-14
影像设备都有什么？CT,DR,乳腺机,谁能一一的介绍下它们的用途呢？在网上找复制过来也可以，我找不到它们的用途。。最好能简单介绍下。我是做医疗设备的新手。。问同行，他们看不起我，甚至连大概价格都不给我说
在图的照相机.反光镜.放大镜.投影仪四个光学仪器中.与其它三个成像...123
大漠11065蛋疼2018-01-14
在图的照相机、反光镜、放大镜、投影仪四个光学仪器中，与其它三个成像原理不同的是（ ）A．B．C．D．
【求助】凝胶成像系统的问题 蛋白质和糖学技术讨论版论坛123
helysmallivan2021-07-21
玩蛋白的大神们，你们在凝胶成像系统下拍照时是怎么做参照的啊，Marker也看不见啊。。。
关于手术显微镜的成像系统的咨询。 考研交流版123
dxy_8zqy69c82017-03-07
请问成像系统工作站请问那家的产品做得比较好以及专业一些呢？
光声成像 123
██櫩9██2018-01-14
光声信号产生的基本原理是：当用短脉冲激光照射吸收体时，吸收体中的分子吸收光子后，当满足一定的条件时，吸收体分子的电子从低能级跃迁到高能级而处于激发态，而处于激发态的电子极不稳定，当电子从高能级向低能级跃迁时，会以光或热量的形式释放能量。在光声成像应用中通常会选择合适波长的激光作为激发源，使吸收的光子的能量转化为热能的效率最大，通常从光能转化为热能的效率可达到90%以上。释放的热量导致吸收体局部温度升高，温度升高后导致热膨胀而产生压力波，这就是光声信号。因此，光声信号的产生过程就是“光能”－“热能”－“机械能”的转化过程。
图1 光声成像工程 (a)光声信号激发与探测；(b)光声成像实现过程示意图
光声成像过程可以分为三个部分：信号的产生、信号的接收和信号处理及图像重建（见图1）。由于脉冲激光器具有光声转换效率高的优点，因此通常被作为光声成像研究中产生信号的激励源。脉冲激光器发出的激光束照射在待研究组织样品上，由于组织样品的吸收效应，在样品内部形成了与组织光学参数相关的能量沉积分布。由于激光脉宽很窄（ns）吸收的能量不能在短时间内释放，导致瞬间温度变化，从而通过热弹机制转化为热膨胀。周期性热流使周围的介质热胀冷缩而激发超声波，由于这种超声波信号的特殊产生机理，为了区别于其它的超声信号，通常称为光声信号。利用超声探测器接收光声信号并对采集到的信号进行适当地处理和采用相应的图像重建算法，就能够得到样品内部光能量沉积的分布。当保证入射光的均匀性的前提下，光声重建图像与吸收分布具有一一对应的关系。向左转|向右转
图1 光声成像工程 (a)光声信号激发与探测；(b)光声成像实现过程示意图
光声成像过程可以分为三个部分：信号的产生、信号的接收和信号处理及图像重建（见图1）。由于脉冲激光器具有光声转换效率高的优点，因此通常被作为光声成像研究中产生信号的激励源。脉冲激光器发出的激光束照射在待研究组织样品上，由于组织样品的吸收效应，在样品内部形成了与组织光学参数相关的能量沉积分布。由于激光脉宽很窄（ns）吸收的能量不能在短时间内释放，导致瞬间温度变化，从而通过热弹机制转化为热膨胀。周期性热流使周围的介质热胀冷缩而激发超声波，由于这种超声波信号的特殊产生机理，为了区别于其它的超声信号，通常称为光声信号。利用超声探测器接收光声信号并对采集到的信号进行适当地处理和采用相应的图像重建算法，就能够得到样品内部光能量沉积的分布。当保证入射光的均匀性的前提下，光声重建图像与吸收分布具有一一对应的关系。向左转|向右转
显微成像系统及方法123
HailHydra52017-09-13
显微镜是OlympusDP71，弄比例尺的时候出来个“衸”，这是什么鬼啊？如何换算成微米？测量的那个选项我选的是10微米，也只有那个选项可以点。然后倍数选择200，然后他就出来这个200衸，选100倍数是500衸，400倍是200也衸，但长度是200的两倍，**各位大神帮帮忙，要怎样才能换成微米？另外1衸是多少微米？
总局关于发布医用磁共振成像系统临床评价等4项医疗器械注册技术...123
eileenpumc2021-07-20
总局关于发布医用磁共振成像系统临床评价等4项医疗器械注册技术审查指导原则的通告（2017年第6号）
2017年01月16日发布http://www.sfda.gov.cn/WS01/CL0087/168596.html
为加强医疗器械产品注册工作的监督和指导，进一步提高注册审查质量，国家食品药品监督管理总局组织制定了《医用磁共振成像系统临床评价技术审查指导原则》《口腔颌面锥形束计算机体层摄影设备注册技术审查指导原则》《体外除颤产品注册技术审查指导原则》《光固化机注册技术审查指导原则》（见附件），现予发布。
特此通告。
附件：1.医用磁共振成像系统临床评价技术审查指导原则
2.口腔颌面锥形束计算机体层摄影设备注册技术审查指导原则
3.体外除颤产品注册技术审查指导原则
4.光固化机注册技术审查指导原则
食品药品监管总局
2017年1月10日
2017年第6号通告附件1.docx
2017年第6号通告附件2.docx
2017年第6号通告附件3.doc
2017年第6号通告附件4.docx
春风一度共缠情第345章123
2018-01-14
通过对人体产生的热量进行捕捉 在呈现出来因而可以穿墙 整体浇筑的建筑应该是将外溢的热量封闭了才无效的把
CAMAG TLC Visualizer 2 薄层色谱数码成像系统123
wuminjie0022016-11-24
实验室新组建，想咨询一下Camag薄层色谱成像系统的价格及一套显微成像的设备清单与价格。显微成像主要用于中药材的显微鉴别
热像仪_红外热像仪_红外热像仪厂家 | Fluke | 福禄克123
2018-01-14
我也最近才了解到，fluke红外热成像仪很抗摔，都说2米跌落都没问题！而且还并经过严苛的振动、电磁干扰、极温、高湿度环境的耐损测试，而市场上的其它产/品却无法证明其能够适用于同等恶劣环境检测。所以还是选择坚固耐用的福禄克靠谱！
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