Antigen Information
- P01106
- 4609
- BHLHE39
- MYC
- Human
Assay Format
- HER/ErbB Signaling
- IGF Signaling
- JAK/STAT Signaling
- MAPK Signaling
- Notch Signaling
- Wnt/beta-Catenin Signaling
- Human
- Cell Lysates
- Nuclear Extracts
- Sandwich-based
- Semi-Quantitative
Product Specifications
Introduction
Product Features
- Specific transcription factor-DNA binding assay
- Perfect alternative to EMSA
- Easy to perform in an ELISA format
- Non-radioactive assay
- High throughput (96-well plate format)
- Assay can be completed within 5 hours
Application Notes
- 96-well Strip Microplate pre-coated with DNA probes
- DNA Binding Buffer
- Positive Control Sample
- Specific Competitor DNA probe
- Non-specific Competitor DNA probe
- Assay Reagent
- DTT
- Wash Buffer
- Primary Antibody
- HRP-conjugated Secondary Antibody
- Antibody Diluent Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Distilled or deionized water
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Absorbent paper
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation < li="">
- Microplate reader capable of measuring absorbance at 450 nm
- Prepare all reagents and samples as instructed in the manual.
- Add 100 µl of sample or positive control to each well.
- Incubate 2 h at RT or O/N at 4 °C.
- Add 100 µl of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared HRP-secondary antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Typical Data
Figure 1Transcription factor assay of c-Myc from nuclear extracts of Jurkat cells or U937 cells. A. Western-blot result of c-Myc from cytoplasm and nuclear fractions. B. Transcription factor assay of c-Myc from nuclear fractions with the RayBio® c-Myc TF Activity Assay.

Figure 2Transcription factor assay of c-Myc from nuclear extracts of Jurkat cells or U937 cells with the specific competitor or non-specific competitor. The result shows specific binding of c-Myc to the DNA binding site.

Storage/Stability
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
加trizol,之后,还需要用玻璃匀浆器磨么?
个人觉得没有必要,如果需要的话是为什么呢?是怕组织没有完全破碎么?
谢谢大家了!



