
Product Name | SensoLyte ® 520 Calpain Activity Assay Kit *Fluorimetric* |
Size | 1 kit |
Catalog # | AS-72149 |
US$ | $526 |
The calpains are a family of intracellular Ca2+ dependent cysteine proteases. The best-characterized calpains are isoforms 1 (μ) and 2 (m). They respond to Ca2+ signals by cleaving many specific proteins, thereby irreversibly modifying their function(s). Calpains are implicated in a variety of Ca2+ regulated cellular processes as well as various pathological phenomena such as ischemic injury, muscular dystrophy, diabetes, cataract, atherosclerosis, Alzheimer"s disease, and cancer. Calpains represent potential therapeutic targets for drug discovery. The SensoLyte® 520 Calpain Activity Assay Kit is optimized for detecting calpain activity. This kit contains a novel internally quenched 5-FAM/QXL® 520 FRET substrate. Calpain protease cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490/520 nm.Increase in fluorescence is proportional to the calpain activity. The assay can detect both calpain 1 (μ) and 2 (m) activities and is ideal for kinetic study of these enzymes. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assays are performed in a convenient 96-well microplate format.Kit size: 100 assaysRELATED PRODUCT:SensoLyte® AMC Calpain Activity Assay Kit (Ex/Em=354/442 nm) | |
Detailed Information | ![]() ![]() ![]() |
Product Citations | Jock, M. et al. (2014). Effect of partial outlet obstruction and reversal on rabbit bladder physiology and biochemistry: Duration of recovery and severity of function. BJU Intl doi: 10.1111/bju.12687.Kawashima, I. et al. (2014). Targeted disruption of Nrg1 in granulosa cells alters the temporal progression of oocyte maturation. Mol Endo 28, 706.Callaghan, CM. et al. (2013). The effect of partial outlet obstruction on calpain and phospholipase-2 activities: analyzed by severity and duration. Mol Cell Biochem 381, 217. doi: 10.1007/s11010-013-1705-8.Kawashima, I. et al. (2012). EGF-Like factors induce expansion of the cumulus cell-oocyte complexes by activating calpain-mediated cell movement. Endocrinol 153, 3949. doi: 10.1210/en.2012-1059.Novgorodov, S. et al. (2011). Developmentally Regulated Ceramide Synthase 6 Increases Mitochondrial Ca2+ Loading Capacity and Promotes Apoptosis. J Biol Chem 286, 4644. |
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有多少种不同类型的震荡仪,型号是什么?
谢谢!
2.溶氧,在好氧培养过程中,空气是滤过开放的,所以通过摇到可以让更多空气中氧气溶解于发酵液中。
厌氧则不是这个作用了。
3.体系均一,有便于对不同参数的取样测定。
前几天我在稀释引物时,被老师看到我正在用漩涡振荡器震荡稀释过的100uM的引物,老师说不能强烈震荡,容易把DNA振断,那请问大家该用什么方法充分混匀?
另有一个问题:2OD的引物,是(1)全部稀释成5uM后4度保存还是(2)稀释成100uM先保存,等到用的时候再稀释成5uM?哪个更好?谢谢大家
推荐一个给你吧《比朗商城》
百度搜索一下! 服务不错的!
2、筛框:由松木或变形量。
Western、IP 等。主要成分为50mM Tris (pH7.4),150mM NaCl,1% NP-40, 0.1% SDS。
使用本品裂解得到的蛋白样品,可以用BCA 蛋白浓度测定试剂盒测定蛋白浓度。由于含有
较高浓度的去垢剂,不能用Bradford 法测定由本裂解液裂解得到样品的蛋白浓度。
保存条件:4℃
制备细胞裂解产物:
1、800g 4℃离心5 分钟,收集细胞,估计细胞离心后的体积(PCV,106 cells ≈ 20ul PCV,
107 cells ≈ 100 ul PCV)
2、每50~100ul PCV 加入5 倍体积的蛋白裂解液(250~500ul),冰浴中放置10 分钟,且每隔5
分钟在漩涡混合仪震荡30 秒;
3、12000g 4℃离心10 分钟,将上清转移到新的离心管中,即得细胞总蛋白产物;
4、假如所得蛋白产物较为粘稠,可95℃加热5 分钟,然后迅速冰浴5 分钟,12000g 4℃离心
10 分钟,将上清转移到新的离心管中,即得细胞总蛋白产物。
制备组织裂解产物:
1、取50-100mg 组织在冰上剪成碎片,用预冷的PBS 洗涤2 次,离心弃去PBS;
2、加入0.5-1ml 预冷的蛋白裂解液;
3、4℃用玻璃匀浆器匀浆20-40 次,直到95%的细胞被破碎,然后在冰浴中放置10 分钟,且每
隔5 分钟在漩涡混合仪震荡30 秒;
4、12000g 4℃离心10 分钟,将上清转移到新的离心管中,即得组织总蛋白产物;
5、假如所得蛋白产物较为粘稠,可95℃加热5 分钟,然后迅速冰浴5 分钟,12000g 4℃离心
10 分钟,将上清转移到新的离心管中,即得组织总蛋白产物。
6、20%的甘油保存于-70℃或-20℃。

